MZF1-AS1 promotes proliferation and invasion of lung adenocarcinoma cells by regulating the miR-27b-3p/KRAS axis.
4 affiliations
- 1Department of Oncology, Longyan First Affiliated Hospital of Fujian Medical University, Fujian, 364000, China.
- 2Department of Thoracic Surgery, The First Hospital of China Medical University, Shenyang, 110000, China.
- 3Respiratory Department, Rugao People's Hospital, Rugao, 226500, China.
- 4Thoracic Surgery, Sun Yat-sen University Cancer Center Gansu Hospital, No.2, Xiaoxihu East Street, Lanzhou, 730050, China. [email protected].
Abstract
Objective
Lung adenocarcinoma (LUAD) has a poor prognosis. MZF1-AS1 is highly expressed in lung cancer tissues. This study aims to examine the prognostic role of MZF1-AS1 in LUAD and the molecular mechanisms by which it regulates the miR-27b-3p/KRAS axis.Methods
This study enrolled 136 patients with LUAD and conducted a 5-year follow-up after treatment. The expression levels of MZF1-AS1, miR-27b-3p, and KRAS were measured in tumor and normal tissues from LUAD patients, as well as in various cell lines. The prognostic value of MZF1-AS1 was evaluated using Cox survival analysis and Kaplan-Meier curve. The cell counting kit-8 (CCK-8) assay and Transwell assay were used to assess cell migration, proliferation and invasion capabilities, respectively. Dual-luciferase reporter (DLR) was used to validate the target-binding relationships between MZF1-AS1 and miR-27b-3p, as well as between miR-27b-3p and KRAS.Results
MZF1-AS1 is upregulated in LUAD tissues and serves as an independent risk factor for LUAD prognosis. In cells, knocking down MZF1-AS1 reduces cancer cell migration, proliferation, and invasion. MZF1-AS1 binds to miR-27b-3p via a competing endogenous RNA (ceRNA) network. miR-27b-3p is downregulated in LUAD tissues and cells; inhibition of miR-27b-3p reverses the protective effects of MZF1-AS1 knockdown. KRAS is a direct target of miR-27b-3p and is upregulated in LUAD tissues. MZF1-AS1 binds to miR-27b-3p, thereby lifting its inhibitory effect on KRAS and promoting cell proliferation and invasion.Conclusion
MZF1-AS1 promotes the growth and invasion of LUAD cell lines by regulating the miR-27b-3p/KRAS axis. This suggests that MZF1-AS1 could be a useful prognostic marker and a potential therapeutic target for LUAD.Clinical trial number
Not applicable.
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BibTeX
@article{lu2026mzf1as1,
title = {{MZF1-AS1 promotes proliferation and invasion of lung adenocarcinoma cells by regulating the miR-27b-3p/KRAS axis.}},
author = {Cuiping Lu and Qixiang Zhong and Haifeng Kan and Jijun Xue},
journal = {Biology direct},
year = {2026},
month = aug,
doi = {10.1186/s13062-026-00936-8},
url = {https://europepmc.org/article/MED/43010020},
note = {CC BY-NC-ND},
}
RIS
TY - JOUR
TI - MZF1-AS1 promotes proliferation and invasion of lung adenocarcinoma cells by regulating the miR-27b-3p/KRAS axis.
AU - Cuiping Lu
AU - Qixiang Zhong
AU - Haifeng Kan
AU - Jijun Xue
PY - 2026
DA - 2026/08/14
JO - Biology direct
DO - 10.1186/s13062-026-00936-8
UR - https://europepmc.org/article/MED/43010020
AB - Objective Lung adenocarcinoma (LUAD) has a poor prognosis. MZF1-AS1 is highly expressed in lung cancer tissues. This study aims to examine the prognostic role of MZF1-AS1 in LUAD and the molecular mechanisms by which it regulates the miR-27b-3p/KRAS axis.Methods This study enrolled 136 patients with LUAD and conducted a 5-year follow-up after treatment. The expression levels of MZF1-AS1, miR-27b-3p, and KRAS were measured in tumor and normal tissues from LUAD patients, as well as in various cell lines. The prognostic value of MZF1-AS1 was evaluated using Cox survival analysis and Kaplan-Meier curve. The cell counting kit-8 (CCK-8) assay and Transwell assay were used to assess cell migration, proliferation and invasion capabilities, respectively. Dual-luciferase reporter (DLR) was used to validate the target-binding relationships between MZF1-AS1 and miR-27b-3p, as well as between miR-27b-3p and KRAS.Results MZF1-AS1 is upregulated in LUAD tissues and serves as an independent risk factor for LUAD prognosis. In cells, knocking down MZF1-AS1 reduces cancer cell migration, proliferation, and invasion. MZF1-AS1 binds to miR-27b-3p via a competing endogenous RNA (ceRNA) network. miR-27b-3p is downregulated in LUAD tissues and cells; inhibition of miR-27b-3p reverses the protective effects of MZF1-AS1 knockdown. KRAS is a direct target of miR-27b-3p and is upregulated in LUAD tissues. MZF1-AS1 binds to miR-27b-3p, thereby lifting its inhibitory effect on KRAS and promoting cell proliferation and invasion.Conclusion MZF1-AS1 promotes the growth and invasion of LUAD cell lines by regulating the miR-27b-3p/KRAS axis. This suggests that MZF1-AS1 could be a useful prognostic marker and a potential therapeutic target for LUAD.Clinical trial number Not applicable.
SN - 1745-6150
ER -