Haplotype assembly without parental sequencing: Genotype-based trio-binning (GT-Trio)
- 1Norwegian University of Life Sciences
Abstract
Trio-binning is a robust method for haplotype-separated assembly, providing the most accurate representation of diploid genomes including complex and haplotype-specific variation. Conventional trio-binning methods depend on parental short-read sequences to differentiate offspring reads originating from the maternal and paternal haplotypes. Here, we present a genotype-based trio-binning pipeline (GT-Trio) which reconstructs parent sequences based on phased parental genotypes and uses this as an alternative source of parental information for haplotype assembly. The GT-Trio pipeline was applied to assemble the maternal and paternal haplotypes of three Norwegian Red cattle individuals, using either imputed sequence variants or SNP array genotypes as input. Haplotypes assembled with GT-Trio using sequence variants as parental input demonstrated high phasing accuracy, similar to that achieved with conventional trio-binning. Using lower-density panels of array SNPs as input resulted in a decrease in phasing accuracy, but still substantially improved haplotype resolution compared to trio-free assembly. We show that the use of high-density parental information from short-reads or sequence variants increases the level of fragmentation in repeat-rich and low heterozygosity regions. Using lower-density parental information from array resolves the contiguity in these regions, but at the expense of phasing accuracy. Overall, GT-Trio provides a robust and scalable framework for haplotype assembly without parental sequencing and is available at https://github.com/theahettasch/GT-Trio.
Related
- Haplotype-resolved genetic mapping using selfed progeny enables simultaneous genome assembly validation and trait dissection in a heterozygous plant
- The haplotype-resolved T2T genome assembly reveals the evolution and adaptation mechanisms of cold resistance in Rosa beggeriana.
- Haplotype-phased, chromosome-level, annotated genome assembly of the soybean rust pathogen Phakopsora pachyrhizi
- A haplotype-resolved, near telomere-to-telomere genome assembly of the desiccation-tolerant plant Vellozia tubiflora
- Somatic haplotype reconstruction and variant recalibration from tumor-only long-read sequencing
BibTeX
@misc{hettasch2026haplotype,
title = {{Haplotype assembly without parental sequencing: Genotype-based trio-binning (GT-Trio)}},
author = {T. J. Hettasch and A. B. Gjuvsland and M. P. Kent and H. Grove and D. I. Vage},
howpublished = {bioRxiv},
year = {2026},
month = jun,
doi = {10.64898/2026.06.08.729486},
url = {https://www.biorxiv.org/content/10.64898/2026.06.08.729486v2},
note = {CC BY},
}
RIS
TY - UNPB
TI - Haplotype assembly without parental sequencing: Genotype-based trio-binning (GT-Trio)
AU - T. J. Hettasch
AU - A. B. Gjuvsland
AU - M. P. Kent
AU - H. Grove
AU - D. I. Vage
PY - 2026
DA - 2026/06/08
PB - bioRxiv
DO - 10.64898/2026.06.08.729486
UR - https://www.biorxiv.org/content/10.64898/2026.06.08.729486v2
AB - Trio-binning is a robust method for haplotype-separated assembly, providing the most accurate representation of diploid genomes including complex and haplotype-specific variation. Conventional trio-binning methods depend on parental short-read sequences to differentiate offspring reads originating from the maternal and paternal haplotypes. Here, we present a genotype-based trio-binning pipeline (GT-Trio) which reconstructs parent sequences based on phased parental genotypes and uses this as an alternative source of parental information for haplotype assembly. The GT-Trio pipeline was applied to assemble the maternal and paternal haplotypes of three Norwegian Red cattle individuals, using either imputed sequence variants or SNP array genotypes as input. Haplotypes assembled with GT-Trio using sequence variants as parental input demonstrated high phasing accuracy, similar to that achieved with conventional trio-binning. Using lower-density panels of array SNPs as input resulted in a decrease in phasing accuracy, but still substantially improved haplotype resolution compared to trio-free assembly. We show that the use of high-density parental information from short-reads or sequence variants increases the level of fragmentation in repeat-rich and low heterozygosity regions. Using lower-density parental information from array resolves the contiguity in these regions, but at the expense of phasing accuracy. Overall, GT-Trio provides a robust and scalable framework for haplotype assembly without parental sequencing and is available at https://github.com/theahettasch/GT-Trio.
ER -