Depletion of border-associated macrophages impairs CSF flow and exacerbates extranuclear mutant Huntingtin aggregation in the zQ175DN mouse model of Huntington’s disease
Abstract
Huntington’s disease (HD) is a neurodegenerative disorder driven by a CAG trinucleotide repeat expansion in the huntingtin ( HTT ) gene, resulting in neuronal degeneration. While cell-autonomous mechanisms within neurons have been extensively characterized, how brain waste clearance pathways at the brain’s borders change during disease progression remains poorly understood. In this study, we investigated the structural and functional adaptations of border-associated macrophages (BAMs) in the zQ175DN knock-in mouse model of HD. Immunohistochemical analysis revealed an age-dependent, selective reduction in the CD206⁺ Lyve1⁺ subset of leptomeningeal BAMs in 16-month-old zQ175DN mice, which tightly correlated with a profound impairment in cerebrospinal fluid (CSF) flow velocity and perivascular influx. To determine whether BAMs actively drive this CSF flow, we selectively depleted these populations of macrophages in 10-month-old zQ175DN mice via intracerebroventricular (i.c.v.) injection of clodronate liposomes (CLO). Chemical ablation of BAMs significantly exacerbated global CSF dynamic impairment, indicted by decreasing CSF fluorescent tracer distribution across perivascular parenchymal channels. Crucially, this CSF flow failure resulted in a marked accumulation of extranuclear mutant huntingtin (mHTT) aggregates in the zQ175DN HD mouse brain, while intranuclear inclusions were not significantly altered. Furthermore, Acute BAMs depletion had no impact on microglial or astrocytic activation in the brain, demonstrating that the exacerbation of mHTT load is a byproduct of brain waste clearance dysfunction rather than gliosis. Together, our results indicate that BAMs are non-redundant regulators of CSF flow dynamics at the brain borders, offering a unique non-neuronal mechanism to mHTT accumulation and potential a therapeutic target to combat HD.
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BibTeX
@misc{jiang2026depletion,
title = {{Depletion of border-associated macrophages impairs CSF flow and exacerbates extranuclear mutant Huntingtin aggregation in the zQ175DN mouse model of Huntington’s disease}},
author = {Yanli Jiang and Rahul Sondawle and Helen Moniz and Emily Mu and Wenzhen Duan},
howpublished = {bioRxiv},
year = {2026},
month = sep,
doi = {10.64898/2026.09.24.754172},
url = {https://doi.org/10.64898/2026.09.24.754172},
note = {CC BY-NC-ND 4.0},
}
RIS
TY - UNPB
TI - Depletion of border-associated macrophages impairs CSF flow and exacerbates extranuclear mutant Huntingtin aggregation in the zQ175DN mouse model of Huntington’s disease
AU - Yanli Jiang
AU - Rahul Sondawle
AU - Helen Moniz
AU - Emily Mu
AU - Wenzhen Duan
PY - 2026
DA - 2026/09/24
PB - bioRxiv
DO - 10.64898/2026.09.24.754172
UR - https://doi.org/10.64898/2026.09.24.754172
AB - Huntington’s disease (HD) is a neurodegenerative disorder driven by a CAG trinucleotide repeat expansion in the huntingtin ( HTT ) gene, resulting in neuronal degeneration. While cell-autonomous mechanisms within neurons have been extensively characterized, how brain waste clearance pathways at the brain’s borders change during disease progression remains poorly understood. In this study, we investigated the structural and functional adaptations of border-associated macrophages (BAMs) in the zQ175DN knock-in mouse model of HD. Immunohistochemical analysis revealed an age-dependent, selective reduction in the CD206⁺ Lyve1⁺ subset of leptomeningeal BAMs in 16-month-old zQ175DN mice, which tightly correlated with a profound impairment in cerebrospinal fluid (CSF) flow velocity and perivascular influx. To determine whether BAMs actively drive this CSF flow, we selectively depleted these populations of macrophages in 10-month-old zQ175DN mice via intracerebroventricular (i.c.v.) injection of clodronate liposomes (CLO). Chemical ablation of BAMs significantly exacerbated global CSF dynamic impairment, indicted by decreasing CSF fluorescent tracer distribution across perivascular parenchymal channels. Crucially, this CSF flow failure resulted in a marked accumulation of extranuclear mutant huntingtin (mHTT) aggregates in the zQ175DN HD mouse brain, while intranuclear inclusions were not significantly altered. Furthermore, Acute BAMs depletion had no impact on microglial or astrocytic activation in the brain, demonstrating that the exacerbation of mHTT load is a byproduct of brain waste clearance dysfunction rather than gliosis. Together, our results indicate that BAMs are non-redundant regulators of CSF flow dynamics at the brain borders, offering a unique non-neuronal mechanism to mHTT accumulation and potential a therapeutic target to combat HD.
SN - 2692-8205
ER -