Naoxueshu Oral Liquid Attenuates Early Radiation-Induced Brain Injury in Rats.
Abstract
Objective
To investigate the effect of Naoxueshu Oral Liquid on rats with radiation-induced brain injury.Methods
A total of 108 male Sprague-Dawley rats were randomly assigned to a control group, an irradiated + normal saline group (RBI+NS), and an irradiated + Naoxueshu Oral Liquid group (RBI+NXS). A radiation-induced brain injury model was established by single-dose whole-brain high-energy X-ray irradiation (30 Gy). After irradiation, rats received Naoxueshu Oral Liquid (0.5 mL/100 g/day, gavage) or normal saline for 14 days. Body weight was monitored, cerebral edema was assessed by brain tissue water content on Days 3, 7, and 14, histopathology was evaluated by hematoxylin-eosin (H&E) staining, and Morris water maze training was conducted from Day 8 to Day 13 post-irradiation, followed by the probe trial on Day 14 post-irradiation.Results
Body weight differed significantly among groups after irradiation (Day 3/6/9/12: all p < 0.01). Specifically, at Day 12, body weight was 328.17±20.10 g (control), 258.50±18.87 g (RBI+NS), and 302.83 ± 10.87 g (RBI+NXS); RBI+NXS was higher than RBI+NS (p < 0.01), while control versus RBI+NS remained different (p < 0.01). H&E staining showed widened perineuronal and perivascular spaces after irradiation, which were attenuated by Naoxueshu. In the Morris water maze, RBI+NS rats exhibited prolonged latency and fewer platform crossings; these impairments were improved after Naoxueshu treatment.Conclusion
Naoxueshu Oral Liquid reduced cerebral edema and improved Morris water maze performance in rats after whole-brain irradiation. Because behavioral outcomes were assessed only using the Morris water maze, the cognitive-related findings should be interpreted cautiously.
Related
- Bovine milk-derived osteopontin attenuates hyperoxia-induced lung injury associated with NLRP3 inflammasome regulation in neonatal mice with bronchopulmonary dysplasia
- Empagliflozin attenuates ISO-induced myocardial injury through PGC-1α-mediated mitochondrial biogenesis and improved fatty acid metabolism
- Glycyrrhizic acid attenuates sepsis-induced acute lung injury by regulating macrophage M2 polarization via PRKCD
- TNFSF14 deficiency attenuates the ischemia/reperfusion-induced acute kidney injury-to-chronic kidney disease transition by upregulating PGC-1α expression
- Hydroethanolic Montanoa bipinnatifida Leaf Extract Attenuates Acute Ethanol-Induced Gastric Mucosal Injury in Female CD-1 Mice: Associations with Lipid Peroxidation and IL-1β Expression
BibTeX
@article{li2026naoxueshu,
title = {{Naoxueshu Oral Liquid Attenuates Early Radiation-Induced Brain Injury in Rats.}},
author = {Fuguang Li and Xiaolong Qu and Kuiyuan Li and Guosong Li and Shifang Zhou and Min Zhao and Chunyu Zhang and Lei Hui and Wenchao Li},
journal = {Brain and behavior},
year = {2026},
month = oct,
doi = {10.1002/brb3.71801},
url = {https://europepmc.org/article/MED/42875996},
note = {CC BY},
}
RIS
TY - JOUR
TI - Naoxueshu Oral Liquid Attenuates Early Radiation-Induced Brain Injury in Rats.
AU - Fuguang Li
AU - Xiaolong Qu
AU - Kuiyuan Li
AU - Guosong Li
AU - Shifang Zhou
AU - Min Zhao
AU - Chunyu Zhang
AU - Lei Hui
AU - Wenchao Li
PY - 2026
DA - 2026/10/01
JO - Brain and behavior
DO - 10.1002/brb3.71801
UR - https://europepmc.org/article/MED/42875996
AB - Objective To investigate the effect of Naoxueshu Oral Liquid on rats with radiation-induced brain injury.Methods A total of 108 male Sprague-Dawley rats were randomly assigned to a control group, an irradiated + normal saline group (RBI+NS), and an irradiated + Naoxueshu Oral Liquid group (RBI+NXS). A radiation-induced brain injury model was established by single-dose whole-brain high-energy X-ray irradiation (30 Gy). After irradiation, rats received Naoxueshu Oral Liquid (0.5 mL/100 g/day, gavage) or normal saline for 14 days. Body weight was monitored, cerebral edema was assessed by brain tissue water content on Days 3, 7, and 14, histopathology was evaluated by hematoxylin-eosin (H&E) staining, and Morris water maze training was conducted from Day 8 to Day 13 post-irradiation, followed by the probe trial on Day 14 post-irradiation.Results Body weight differed significantly among groups after irradiation (Day 3/6/9/12: all p < 0.01). Specifically, at Day 12, body weight was 328.17±20.10 g (control), 258.50±18.87 g (RBI+NS), and 302.83 ± 10.87 g (RBI+NXS); RBI+NXS was higher than RBI+NS (p < 0.01), while control versus RBI+NS remained different (p < 0.01). H&E staining showed widened perineuronal and perivascular spaces after irradiation, which were attenuated by Naoxueshu. In the Morris water maze, RBI+NS rats exhibited prolonged latency and fewer platform crossings; these impairments were improved after Naoxueshu treatment.Conclusion Naoxueshu Oral Liquid reduced cerebral edema and improved Morris water maze performance in rats after whole-brain irradiation. Because behavioral outcomes were assessed only using the Morris water maze, the cognitive-related findings should be interpreted cautiously.
SN - 2162-3279
ER -