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Peer-reviewedCC BY-NC-NDBiomedical engineering online

A stratified cell sheet platform coordinating dual osteogenic and chondrogenic differentiation for osteochondral tissue engineering.

  • Fatemeh Leisi Mehrabani1,2
  • Samaneh Hosseini2,3
  • Mohamadreza Baghaban Eslaminejad4
4 affiliations
  1. 1Faculty of Sciences and Advanced Technologies in Biology, University of Science and Culture, Tehran, Iran.
  2. 2Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.
  3. 3Department of Cell Engineering, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.
  4. 4Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran. [email protected].
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Abstract

Stem cell sheets have unique cell-to-cell connections, the ability to enhance and preserve the extracellular matrix (ECM) and cell-ECM interactions, and a scaffold-free nature, which makes them ideal for regenerative medicine. This study aims to explore the potential of stem cell sheets for osteochondral construct fabrication. Mesenchymal stem cells (MSCs) were isolated from rat bone marrow and differentiated into osteogenic and chondrogenic sheets. These differentiated cell sheets were subsequently stacked using a collagen and hyaluronic acid hydrogel between the layers to create multilayered constructs. Histological analysis demonstrated notable tissue formation of a multilayered structure with distinct osteogenic and chondrogenic layers both with and without the hydrogel constructs. Scanning electron microscopy (SEM) confirmed the integrity and content of the sheets. qRT-PCR and immunocytochemistry analysis demonstrated the presence of key related bone and cartilage markers within the differentiated sheets. In conclusion, our data emphasize that stem cell sheet engineering (CSE) holds significant promise for developing 3D, scaffold-free constructs for osteochondral regeneration. The ability to create relatively distinct osteogenic and chondrogenic layers in constructs has an extreme impact on the field of regenerative medicine, leading to a new therapeutic approach and hope for the future of osteochondral defects treatment.

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Cite

BibTeXRIS
BibTeX
@article{mehrabani2026stratified,
  title = {{A stratified cell sheet platform coordinating dual osteogenic and chondrogenic differentiation for osteochondral tissue engineering.}},
  author = {Fatemeh Leisi Mehrabani and Samaneh Hosseini and Mohamadreza Baghaban Eslaminejad},
  journal = {Biomedical engineering online},
  year = {2026},
  month = aug,
  doi = {10.1186/s12938-026-01609-3},
  url = {https://europepmc.org/article/MED/42958351},
  note = {CC BY-NC-ND},
}
RIS
TY  - JOUR
TI  - A stratified cell sheet platform coordinating dual osteogenic and chondrogenic differentiation for osteochondral tissue engineering.
AU  - Fatemeh Leisi Mehrabani
AU  - Samaneh Hosseini
AU  - Mohamadreza Baghaban Eslaminejad
PY  - 2026
DA  - 2026/08/01
JO  - Biomedical engineering online
DO  - 10.1186/s12938-026-01609-3
UR  - https://europepmc.org/article/MED/42958351
AB  - Stem cell sheets have unique cell-to-cell connections, the ability to enhance and preserve the extracellular matrix (ECM) and cell-ECM interactions, and a scaffold-free nature, which makes them ideal for regenerative medicine. This study aims to explore the potential of stem cell sheets for osteochondral construct fabrication. Mesenchymal stem cells (MSCs) were isolated from rat bone marrow and differentiated into osteogenic and chondrogenic sheets. These differentiated cell sheets were subsequently stacked using a collagen and hyaluronic acid hydrogel between the layers to create multilayered constructs. Histological analysis demonstrated notable tissue formation of a multilayered structure with distinct osteogenic and chondrogenic layers both with and without the hydrogel constructs. Scanning electron microscopy (SEM) confirmed the integrity and content of the sheets. qRT-PCR and immunocytochemistry analysis demonstrated the presence of key related bone and cartilage markers within the differentiated sheets. In conclusion, our data emphasize that stem cell sheet engineering (CSE) holds significant promise for developing 3D, scaffold-free constructs for osteochondral regeneration. The ability to create relatively distinct osteogenic and chondrogenic layers in constructs has an extreme impact on the field of regenerative medicine, leading to a new therapeutic approach and hope for the future of osteochondral defects treatment.
SN  - 1475-925X
ER  -