Skip to content
DraconiticResearch
Search Sources API
203,234 studies31 sourcesUpdated 5 min ago
Peer-reviewedCC BY 4.0BMC Microbiology

A newly Bacillus subtilis isolate for PHB biosynthesis: optimization and thermal spectroscopic validation

  • Afaf Altrawy1
  • Rehab Abdelmonem2
  • Mahmoud W. Sadik3,4
  • Sara K. Mostafa3
  • Shrouk R. Elsaid3
  • Mohamed A. Abd-Elhakeem5
5 affiliations
  1. 1Department of Medical Biotechnology, College of Biotechnology, Misr University for Science and Technology (MUST), Giza, 12566 Egypt
  2. 2Industrial Pharmacy Department, College of Pharmaceutical Sciences and Drug Manufacturing, Misr University for Science and Technology (MUST), 12566 Giza, Egypt
  3. 3Department of Environmental Biotechnology, College of Biotechnology, Misr University for Science and Technology (MUST), 12566 Giza, Egypt
  4. 4Department of Microbiology, Faculty of Agriculture, Cairo University, Giza, 12613 Egypt
  5. 5Department of Pharmaceutical Biotechnology, College of Biotechnology, Misr University for Science and Technology (MUST), 12566 Giza, Egypt
Read full textPDFDOICite

Abstract

Poly(3-hydroxybutyrate) (PHB) is a biodegradable polyester considered a sustainable alternative to conventional plastics. This study aimed to isolate a PHB-producing bacterium from agricultural soil and to evaluate the natural honey produced by Apis mellifera as an alternative carbon source for PHB production. A PHB-producing isolate was obtained and identified as Bacillus subtilis strain MUST-MWS1 based on morphological, biochemical, and 16 S rRNA gene analyses. Cultures were grown at pH 8.2 using LB medium, glucose, or honey as the main carbon source, and PHB was extracted by a chloroform–methanol method. The polymer was characterized by Fourier transform infrared spectroscopy, gas chromatography–mass spectrometry, X-ray diffraction, differential scanning calorimetry, and thermogravimetric analysis. Honey yielded the highest PHB titer (0.2669 ± 0.0078 g/L), compared with glucose (0.2168 ± 0.0068 g/L) and LB medium (0.1933 ± 0.0067 g/L). The structural and thermal properties of the biosynthesized PHB were consistent with those reported for commercial PHB. These findings indicate that B. subtilis MUST-MWS1 is a promising PHB producer and that honey can serve as an effective alternative carbon source for bioplastic production.

Related

  1. Machine Learning-Assisted Optimization of Surfactin Titer Produced by Bacillus subtilis MTCC 2423 from Minimum Fermentation Data with Experimental Validation
    Ashwini Padole, Riju De, Vivek Rangarajan +1
    ACS OmegaPeer-reviewedCC BY 4.0Full textGeneral chemistryCrossref
  2. Cross-Validation of Electrochemical, Spectroscopic, and Gravimetric Methods for Characterizing Self-Assembled Monolayers
    Jiayao Lu, Siyuan L. Xie, Davis Kurdyla +1
    ChemRxivPreprintLicence not statedGeneral chemistryChemRxiv
  3. Metabolic Engineering and Bioprocess Optimization Strategies Promote l‑Azidohomoalanine Biosynthesis in Fed-Batch Bioreactor Culture
    Claudia Lacombe, Gerald Striedner, Birgit Wiltschi
    ACS omegaPeer-reviewedCC BYFull textGeneral chemistryEurope PMC
  4. Optimization and interlaboratory validation of the HydroTouch test for fingertip-mediated microbial transmission and antimicrobial efficacy assessment of surfaces
    Roberto B.M. Marano, Mihyun Lee, Celeste Peterson +11
    BMC MicrobiologyPeer-reviewedCC BY 4.0Full textOrganic chemistryPMC
  5. An Attention-based Architecture to Decode Chemical Transformations from Process Spectroscopic Data
    Kuldeep Singh, Vinay Prasad
    ChemRxivPreprintLicence not statedGeneral chemistryChemRxiv

Cite

BibTeXRIS
BibTeX
@article{altrawy2026newly,
  title = {{A newly Bacillus subtilis isolate for PHB biosynthesis: optimization and thermal spectroscopic validation}},
  author = {Afaf Altrawy and Rehab Abdelmonem and Mahmoud W. Sadik and Sara K. Mostafa and Shrouk R. Elsaid and Mohamed A. Abd-Elhakeem},
  journal = {BMC Microbiology},
  year = {2026},
  month = nov,
  doi = {10.1186/s12866-026-05715-z},
  url = {https://doi.org/10.1186/s12866-026-05715-z},
  note = {CC BY 4.0},
}
RIS
TY  - JOUR
TI  - A newly Bacillus subtilis isolate for PHB biosynthesis: optimization and thermal spectroscopic validation
AU  - Afaf Altrawy
AU  - Rehab Abdelmonem
AU  - Mahmoud W. Sadik
AU  - Sara K. Mostafa
AU  - Shrouk R. Elsaid
AU  - Mohamed A. Abd-Elhakeem
PY  - 2026
DA  - 2026/11/03
JO  - BMC Microbiology
DO  - 10.1186/s12866-026-05715-z
UR  - https://doi.org/10.1186/s12866-026-05715-z
AB  - Poly(3-hydroxybutyrate) (PHB) is a biodegradable polyester considered a sustainable alternative to conventional plastics. This study aimed to isolate a PHB-producing bacterium from agricultural soil and to evaluate the natural honey produced by Apis mellifera as an alternative carbon source for PHB production. A PHB-producing isolate was obtained and identified as Bacillus subtilis strain MUST-MWS1 based on morphological, biochemical, and 16 S rRNA gene analyses. Cultures were grown at pH 8.2 using LB medium, glucose, or honey as the main carbon source, and PHB was extracted by a chloroform–methanol method. The polymer was characterized by Fourier transform infrared spectroscopy, gas chromatography–mass spectrometry, X-ray diffraction, differential scanning calorimetry, and thermogravimetric analysis. Honey yielded the highest PHB titer (0.2669 ± 0.0078 g/L), compared with glucose (0.2168 ± 0.0068 g/L) and LB medium (0.1933 ± 0.0067 g/L). The structural and thermal properties of the biosynthesized PHB were consistent with those reported for commercial PHB. These findings indicate that B. subtilis MUST-MWS1 is a promising PHB producer and that honey can serve as an effective alternative carbon source for bioplastic production.
SN  - 1471-2180
ER  -