The effect of indomethacin and bone wax on preventing bony bridging after growth plate injury
- 1Department of Orthopedic Surgery, Ege University Faculty of Medicine, İzmir, Türkiye
Abstract
Objective: This study aimed to investigate the efficacy of indomethacin and bone wax, individually and in combination, in preventing bony bridge formation following growth plate injury in a rat model.
Methods: Fifteen skeletally immature male Wistar rats were randomly allocated into 5 groups: an uninjured control, an injured control, an isolated bone wax group, an isolated indomethacin group, and a combination group receiving both bone wax and indomethacin. Following standardized growth plate injuries to the proximal tibia, the respective interventions were applied. On day 28, bone volume fraction (BV/TV%) and bone mineral density (BMD) were calculated using micro-computed tomography (micro-CT). Data distribution was assessed with the Shapiro–Wilk test, and the 5 groups were compared using the Kruskal–Wallis test; values are reported as median (interquartile range [IQR]), with statistical significance set at P < .05.
Results: All treatment groups showed lower BV/TV% and BMD than the control groups, with the combined bone wax and indomethacin group showing the lowest values. Median BV/TV% was 52.49% (IQR = 14.92-70.71) in the combined group vs. 75.63% (IQR = 69.64-77.12) in the injured control group, and median BMD was 14.56 mg/cm3 (IQR = 9.73-26.35) vs. 128.03 mg/cm3 (IQR = 11.93-184.02), respectively. On Kruskal–Wallis testing, however, these differences were not statistically significant (BV/TV% = P = .513; BMD: P = .247).
Conclusion: The combined application of systemic indomethacin and bone wax interposition showed the lowest values for both bone volume fraction and BMD, which may reflect a potential additive effect of attenuating inflammation-driven osteogenesis and physically blocking bony bridging. However, the absence of significant differences underscores the need for further research and clinical trials before their routine clinical application can be recommended.
Cite this article as: Aljasim O, Kayikçi K, Vahabi A, Kaya H, Günay H. The effect of indomethacin and bone wax on preventing bony bridging after growth plate injury. Acta Orthop Traumatol Turc.,2026; 60(5), 0783, doi: 10.5152/j.aott.2026.26783.
Related
- Comparison of indomethacin and nifedipine regarding ductus arteriosus Doppler findings after laser therapy for monochorionic diamniotic twin-to-twin transfusion syndrome: A retrospective cohort study
- Bridging the gap in maternal care: the role of community-based workers in preventing maternal mortality
- Trial sequential analysis of the efficacy of amino acid and protein therapy in preventing cardiac surgery-associated acute kidney injury: a systematic review and meta-analysis
- Preventing acute kidney injury in high-risk patients
- Statistical Analysis Plan for the REDEP study: Effectiveness of dual-mobility cups for preventing dislocation after primary total hip arthroplasty by a posterolateral approach compared to unipolar cups in elderly patients
BibTeX
@article{aljasim2026effect,
title = {{The effect of indomethacin and bone wax on preventing bony bridging after growth plate injury}},
author = {Omar Aljasim and Kayahan Kayıkcı and Arman Vahabi and Hüseyin Kaya and Hüseyin Günay},
journal = {Acta Orthopaedica et Traumatologica Turcica},
year = {2026},
month = oct,
doi = {10.5152/j.aott.2026.26783},
url = {https://doi.org/10.5152/j.aott.2026.26783},
note = {CC BY-NC},
}
RIS
TY - JOUR
TI - The effect of indomethacin and bone wax on preventing bony bridging after growth plate injury
AU - Omar Aljasim
AU - Kayahan Kayıkcı
AU - Arman Vahabi
AU - Hüseyin Kaya
AU - Hüseyin Günay
PY - 2026
DA - 2026/10/23
JO - Acta Orthopaedica et Traumatologica Turcica
DO - 10.5152/j.aott.2026.26783
UR - https://doi.org/10.5152/j.aott.2026.26783
AB - Objective: This study aimed to investigate the efficacy of indomethacin and bone wax, individually and in combination, in preventing bony bridge formation following growth plate injury in a rat model. Methods: Fifteen skeletally immature male Wistar rats were randomly allocated into 5 groups: an uninjured control, an injured control, an isolated bone wax group, an isolated indomethacin group, and a combination group receiving both bone wax and indomethacin. Following standardized growth plate injuries to the proximal tibia, the respective interventions were applied. On day 28, bone volume fraction (BV/TV%) and bone mineral density (BMD) were calculated using micro-computed tomography (micro-CT). Data distribution was assessed with the Shapiro–Wilk test, and the 5 groups were compared using the Kruskal–Wallis test; values are reported as median (interquartile range [IQR]), with statistical significance set at P < .05. Results: All treatment groups showed lower BV/TV% and BMD than the control groups, with the combined bone wax and indomethacin group showing the lowest values. Median BV/TV% was 52.49% (IQR = 14.92-70.71) in the combined group vs. 75.63% (IQR = 69.64-77.12) in the injured control group, and median BMD was 14.56 mg/cm3 (IQR = 9.73-26.35) vs. 128.03 mg/cm3 (IQR = 11.93-184.02), respectively. On Kruskal–Wallis testing, however, these differences were not statistically significant (BV/TV% = P = .513; BMD: P = .247). Conclusion: The combined application of systemic indomethacin and bone wax interposition showed the lowest values for both bone volume fraction and BMD, which may reflect a potential additive effect of attenuating inflammation-driven osteogenesis and physically blocking bony bridging. However, the absence of significant differences underscores the need for further research and clinical trials before their routine clinical application can be recommended. Cite this article as: Aljasim O, Kayikçi K, Vahabi A, Kaya H, Günay H. The effect of indomethacin and bone wax on preventing bony bridging after growth plate injury. Acta Orthop Traumatol Turc.,2026; 60(5), 0783, doi: 10.5152/j.aott.2026.26783.
SN - 1017-995X
SN - 2589-1294
ER -