Dietary hot red pepper powder modulates laying performance, hematology profile, and hepatic antioxidant status in laying hens under tropical conditions.
- 1Department of Animal Production, Faculty of Animal Science, Universitas Gadjah Mada, Jalan Fauna No. 3, Yogyakarta, 55281, Indonesia.
- 2Department of Animal Nutrition and Feed Science, Faculty of Animal Science, Universitas Gadjah Mada, Jalan Fauna No. 3, Yogyakarta, 55281, Indonesia.
Abstract
Hot red pepper (HRP) has emerged as a phytogenic feed additive with the potential to enhance laying performance in laying hens by improving gut health and mitigating oxidative stress. This study aimed to evaluate the effects of dietary inclusion of HRP powder on the productive performance, hematological and biochemical profiles, intestinal morphology, oxidative stress markers, and liver histopathology of laying hens reared under tropical conditions. A total of 100 Novogen laying hens were randomly assigned to four dietary treatments for eight weeks: a basal control diet and diets supplemented with 0.25%, 0.5%, or 0.75% HRP powder. Dietary supplementation with 0.25% and 0.5% HRP improved hen-day egg production during the first period (P < 0.05). HRP inclusion significantly reduced serum cholesterol levels (P < 0.05). Additionally, HRP supplementation (0.25-0.75%) increased lymphocyte counts (P < 0.001) while decreasing total leukocytes, heterophils, and the heterophil-to-lymphocyte (H/L) ratio (P < 0.001). Regarding gut environment, dietary inclusion of 0.5%-0.75% HRP decreased caecal pH, whereas 0.75% HRP reduced colon pH (P < 0.05). For antioxidant status, HRP at 0.25-0.5% enhanced hepatic superoxide dismutase activity (P < 0.05), while all inclusion levels (0.5-0.75%) lowered malondialdehyde concentrations (P < 0.05). Histopathological evaluation confirmed reduced liver inflammation and cellular degeneration. Overall, dietary HRP improves productive performance, physiological status, gut function, and liver health in laying hens under tropical conditions, with 0.5% providing the most consistent benefits, supporting its application as an effective phytogenic feed additive in poultry production.
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BibTeX
@article{hanif2026dietary,
title = {{Dietary hot red pepper powder modulates laying performance, hematology profile, and hepatic antioxidant status in laying hens under tropical conditions.}},
author = {Muhammad Fathin Hanif and Ali Agus and Bambang Ariyadi and Muhlisin and Nanung Danar Dono and Heru Sasongko and Chusnul Hanim},
journal = {Veterinary and animal science},
year = {2026},
month = oct,
doi = {10.1016/j.vas.2026.100891},
url = {https://europepmc.org/article/MED/42903682},
note = {CC BY},
}
RIS
TY - JOUR
TI - Dietary hot red pepper powder modulates laying performance, hematology profile, and hepatic antioxidant status in laying hens under tropical conditions.
AU - Muhammad Fathin Hanif
AU - Ali Agus
AU - Bambang Ariyadi
AU - Muhlisin
AU - Nanung Danar Dono
AU - Heru Sasongko
AU - Chusnul Hanim
PY - 2026
DA - 2026/10/06
JO - Veterinary and animal science
DO - 10.1016/j.vas.2026.100891
UR - https://europepmc.org/article/MED/42903682
AB - Hot red pepper (HRP) has emerged as a phytogenic feed additive with the potential to enhance laying performance in laying hens by improving gut health and mitigating oxidative stress. This study aimed to evaluate the effects of dietary inclusion of HRP powder on the productive performance, hematological and biochemical profiles, intestinal morphology, oxidative stress markers, and liver histopathology of laying hens reared under tropical conditions. A total of 100 Novogen laying hens were randomly assigned to four dietary treatments for eight weeks: a basal control diet and diets supplemented with 0.25%, 0.5%, or 0.75% HRP powder. Dietary supplementation with 0.25% and 0.5% HRP improved hen-day egg production during the first period (P < 0.05). HRP inclusion significantly reduced serum cholesterol levels (P < 0.05). Additionally, HRP supplementation (0.25-0.75%) increased lymphocyte counts (P < 0.001) while decreasing total leukocytes, heterophils, and the heterophil-to-lymphocyte (H/L) ratio (P < 0.001). Regarding gut environment, dietary inclusion of 0.5%-0.75% HRP decreased caecal pH, whereas 0.75% HRP reduced colon pH (P < 0.05). For antioxidant status, HRP at 0.25-0.5% enhanced hepatic superoxide dismutase activity (P < 0.05), while all inclusion levels (0.5-0.75%) lowered malondialdehyde concentrations (P < 0.05). Histopathological evaluation confirmed reduced liver inflammation and cellular degeneration. Overall, dietary HRP improves productive performance, physiological status, gut function, and liver health in laying hens under tropical conditions, with 0.5% providing the most consistent benefits, supporting its application as an effective phytogenic feed additive in poultry production.
SN - 2451-943X
ER -