Bivalency of natalizumab promotes inhibition of dynamic VLA-4 adhesion beyond affinity gain
Abstract
Natalizumab, a monoclonal IgG4 antibody used in the treatment of multiple sclerosis (MS), inhibits α4β1 (very late antigen-4, VLA-4)–binding to vascular cell adhesion molecule-1 (VCAM-1), thereby reducing leukocyte recruitment to inflamed tissues. The intricate mechanisms underlying these effects remain unclear, particularly concerning the heavy-chain shuffling of IgG4. We conducted an in vitro study to quantify the impact of bivalent IgG and monovalent Fab forms of natalizumab on the capture and migration of human primary memory T lymphocytes under shear stress, using VCAM-1 and CXCL12-coated surfaces. IgG natalizumab at concentrations near its cell surface EC50 (half-maximal effective concentration) showed significant of capture and resistance of adherent cells to shear stress, whereas significantly higher doses of Fab natalizumab, up to 100-fold greater than its cell surface EC50, were needed to achieve similar effects. These findings highlight that receptor occupancy alone may not adequately predict the functional outcomes of inhibitor antibodies. For optimal therapeutic effect, inhibition of cell-surface adhesion may require specific kinetic and geometric binding properties of antibodies. Insight Box This study shows how natalizumab functionally inhibits α4β1 (very late antigen-4, VLA-4)–mediated T-cell adhesion by integrating quantitative affinity measurements with dynamic assays performed under shear stress. We show that the antibody’s bivalency provides a major functional advantage, enabling strong inhibition of cell capture and adhesion at concentrations near its EC50 (half-maximal effective concentration), whereas monovalent Fab fragments require 10- to 100-fold higher doses. These findings offer biological insight into why receptor occupancy alone cannot predict adhesion inhibition. By combining cell-based affinity quantification with real-time adhesion and migration assays, our work reveals how kinetic and geometric properties of antibody–integrin interactions shape immune cell dynamics, informing the rational design of next-generation integrin-targeting therapeutics.
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BibTeX
@article{valignat2026bivalency,
title = {{Bivalency of natalizumab promotes inhibition of dynamic VLA-4 adhesion beyond affinity gain}},
author = {Marie-Pierre Valignat and Martine Biarnes-Pelicot and Dominique Touchard and Audrey Rico-Lamy and Patrick Chames and Olivier Theodoly and Philippe Robert},
journal = {Integrative biology},
year = {2026},
month = jul,
doi = {10.1093/intbio/zyag015},
url = {https://hal.science/hal-05747472v1},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Bivalency of natalizumab promotes inhibition of dynamic VLA-4 adhesion beyond affinity gain
AU - Marie-Pierre Valignat
AU - Martine Biarnes-Pelicot
AU - Dominique Touchard
AU - Audrey Rico-Lamy
AU - Patrick Chames
AU - Olivier Theodoly
AU - Philippe Robert
PY - 2026
DA - 2026/07/31
JO - Integrative biology
DO - 10.1093/intbio/zyag015
UR - https://hal.science/hal-05747472v1
AB - Natalizumab, a monoclonal IgG4 antibody used in the treatment of multiple sclerosis (MS), inhibits α4β1 (very late antigen-4, VLA-4)–binding to vascular cell adhesion molecule-1 (VCAM-1), thereby reducing leukocyte recruitment to inflamed tissues. The intricate mechanisms underlying these effects remain unclear, particularly concerning the heavy-chain shuffling of IgG4. We conducted an in vitro study to quantify the impact of bivalent IgG and monovalent Fab forms of natalizumab on the capture and migration of human primary memory T lymphocytes under shear stress, using VCAM-1 and CXCL12-coated surfaces. IgG natalizumab at concentrations near its cell surface EC50 (half-maximal effective concentration) showed significant of capture and resistance of adherent cells to shear stress, whereas significantly higher doses of Fab natalizumab, up to 100-fold greater than its cell surface EC50, were needed to achieve similar effects. These findings highlight that receptor occupancy alone may not adequately predict the functional outcomes of inhibitor antibodies. For optimal therapeutic effect, inhibition of cell-surface adhesion may require specific kinetic and geometric binding properties of antibodies. Insight Box This study shows how natalizumab functionally inhibits α4β1 (very late antigen-4, VLA-4)–mediated T-cell adhesion by integrating quantitative affinity measurements with dynamic assays performed under shear stress. We show that the antibody’s bivalency provides a major functional advantage, enabling strong inhibition of cell capture and adhesion at concentrations near its EC50 (half-maximal effective concentration), whereas monovalent Fab fragments require 10- to 100-fold higher doses. These findings offer biological insight into why receptor occupancy alone cannot predict adhesion inhibition. By combining cell-based affinity quantification with real-time adhesion and migration assays, our work reveals how kinetic and geometric properties of antibody–integrin interactions shape immune cell dynamics, informing the rational design of next-generation integrin-targeting therapeutics.
SN - 1757-9708
SN - 1757-9708
ER -