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Peer-reviewedCC BYSTAR protocols

Protocol for assessing BMP9-induced angiogenic and osteogenic activity in vitro and in vivo.

  • Jianing Duan1
  • Yuanyuan Zhang1
  • Weida Zhang1
  • Xiaohong Qin2
  • Li-Zhi Mi3
  1. 1School of Life Sciences, Faculty of Medicine, Tianjin University, Tianjin 300072, P.R. China.
  2. 2School of Life Sciences, Faculty of Medicine, Tianjin University, Tianjin 300072, P.R. China; State Key Laboratory of Synthetic Biology, Tianjin University, Tianjin 300072, P.R. China. Electronic address: [email protected].
  3. 3School of Life Sciences, Faculty of Medicine, Tianjin University, Tianjin 300072, P.R. China; State Key Laboratory of Synthetic Biology, Tianjin University, Tianjin 300072, P.R. China. Electronic address: [email protected].
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Abstract

BMP9 is an important regulator in angiogenesis, tissue development, and immune response. Here, we present a protocol to examine the regulatory mechanism of unprocessed BMP9 precursor in BMP9 signaling. We describe procedures for assessing BMP9-induced angiogenic and osteogenic activity in vitro and in vivo. We describe steps for their assessment using flow cytometric analysis, C2C12 differentiation assay, chicken embryo chorioallantoic membrane assay, and mouse subcutaneous Matrigel assay. For complete details on the use and execution of this protocol, please refer to Zhang et al.¹.

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Cite

BibTeXRIS
BibTeX
@article{duan2026protocol,
  title = {{Protocol for assessing BMP9-induced angiogenic and osteogenic activity in vitro and in vivo.}},
  author = {Jianing Duan and Yuanyuan Zhang and Weida Zhang and Xiaohong Qin and Li-Zhi Mi},
  journal = {STAR protocols},
  year = {2026},
  month = oct,
  doi = {10.1016/j.xpro.2026.104894},
  url = {https://europepmc.org/article/MED/42868000},
  note = {CC BY},
}
RIS
TY  - JOUR
TI  - Protocol for assessing BMP9-induced angiogenic and osteogenic activity in vitro and in vivo.
AU  - Jianing Duan
AU  - Yuanyuan Zhang
AU  - Weida Zhang
AU  - Xiaohong Qin
AU  - Li-Zhi Mi
PY  - 2026
DA  - 2026/10/13
JO  - STAR protocols
DO  - 10.1016/j.xpro.2026.104894
UR  - https://europepmc.org/article/MED/42868000
AB  - BMP9 is an important regulator in angiogenesis, tissue development, and immune response. Here, we present a protocol to examine the regulatory mechanism of unprocessed BMP9 precursor in BMP9 signaling. We describe procedures for assessing BMP9-induced angiogenic and osteogenic activity in vitro and in vivo. We describe steps for their assessment using flow cytometric analysis, C2C12 differentiation assay, chicken embryo chorioallantoic membrane assay, and mouse subcutaneous Matrigel assay. For complete details on the use and execution of this protocol, please refer to Zhang et al.¹.
SN  - 2666-1667
ER  -