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Peer-reviewedCC BY 4.0Frontiers in Bioengineering and Biotechnology

Simultaneous enhancement of biogas production and antibiotic resistance gene removal by sawdust biochar in anaerobic digestion of chicken manure

  • Xiaomei Jiang1
  • Ting Shen1,2,3
  • Yunhui Lei1,2,3
  • Xiaobo Guo1,2,3
  1. 1Biogas Institute of Ministry of Agriculture and Rural Affairs, Chengdu, China
  2. 2Key Laboratory of Development and Application of Rural Renewable Energy, Ministry of Agriculture and Rural Affairs, Chengdu, China
  3. 3Research Center for Rural Energy and Ecology, Chinese Academy of Agricultural Sciences, Chengdu, China
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Abstract

This study evaluated the impacts of two types of biochar, namely, corn-stover and sawdust, on biogas yield and the removal of antibiotic resistance genes (ARGs) during the anaerobic digestion (AD) of chicken manure. Corn-stover biochar and sawdust biochar maintained the pH under neutral conditions, and simultaneously, effective hydrolysis, acidification, acetogenesis, and methanogenesis avoided excessive volatile fatty acids (VFAs) and free ammonia (FAN) inhibition, resulting in a 27.69% (corn-stover biochar) and 19.65% (sawdust biochar) increase in cumulative gas production. However, the low specific surface area of sawdust biochar was not conducive to the growth and reproduction of microorganisms, reducing the total number of microorganisms and thus enhancing the removal of macrolide and sulfonamide ARGs’ absolute abundance compared with corn-stover biochar. Microbial community analysis revealed that biochar addition enriched Methanosarcina (up to >96% relative abundance) and syntrophic bacteria such as Sphaerochaeta and Fastidiosipila, which facilitated methanogenesis and substrate degradation. Furthermore, structural equation models (SEMs) revealed that total ammonia nitrogen (TAN), pH, and FAN indirectly determine ARG abundance by influencing potential hosts and intI1. This study can provide a theoretical basis for the application of these common biochars in enhancing the efficiency of chicken manure AD and reducing environmental ARGs.

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Cite

BibTeXRIS
BibTeX
@article{jiang2026simultaneous,
  title = {{Simultaneous enhancement of biogas production and antibiotic resistance gene removal by sawdust biochar in anaerobic digestion of chicken manure}},
  author = {Xiaomei Jiang and Ting Shen and Yunhui Lei and Xiaobo Guo},
  journal = {Frontiers in Bioengineering and Biotechnology},
  year = {2026},
  month = oct,
  doi = {10.3389/fbioe.2026.1897972},
  url = {https://doi.org/10.3389/fbioe.2026.1897972},
  note = {CC BY 4.0},
}
RIS
TY  - JOUR
TI  - Simultaneous enhancement of biogas production and antibiotic resistance gene removal by sawdust biochar in anaerobic digestion of chicken manure
AU  - Xiaomei Jiang
AU  - Ting Shen
AU  - Yunhui Lei
AU  - Xiaobo Guo
PY  - 2026
DA  - 2026/10/21
JO  - Frontiers in Bioengineering and Biotechnology
DO  - 10.3389/fbioe.2026.1897972
UR  - https://doi.org/10.3389/fbioe.2026.1897972
AB  - This study evaluated the impacts of two types of biochar, namely, corn-stover and sawdust, on biogas yield and the removal of antibiotic resistance genes (ARGs) during the anaerobic digestion (AD) of chicken manure. Corn-stover biochar and sawdust biochar maintained the pH under neutral conditions, and simultaneously, effective hydrolysis, acidification, acetogenesis, and methanogenesis avoided excessive volatile fatty acids (VFAs) and free ammonia (FAN) inhibition, resulting in a 27.69% (corn-stover biochar) and 19.65% (sawdust biochar) increase in cumulative gas production. However, the low specific surface area of sawdust biochar was not conducive to the growth and reproduction of microorganisms, reducing the total number of microorganisms and thus enhancing the removal of macrolide and sulfonamide ARGs’ absolute abundance compared with corn-stover biochar. Microbial community analysis revealed that biochar addition enriched Methanosarcina (up to >96% relative abundance) and syntrophic bacteria such as Sphaerochaeta and Fastidiosipila, which facilitated methanogenesis and substrate degradation. Furthermore, structural equation models (SEMs) revealed that total ammonia nitrogen (TAN), pH, and FAN indirectly determine ARG abundance by influencing potential hosts and intI1. This study can provide a theoretical basis for the application of these common biochars in enhancing the efficiency of chicken manure AD and reducing environmental ARGs.
SN  - 2296-4185
ER  -