Whole-body Super-resolution Functional and Molecular Imaging with Panoramic Photoacoustic-Ultrasound Tomography
- 1Junjie Yao
Abstract
Photoacoustic (PA) and ultrasound (US) imaging provide complementary molecular, functional, and anatomical contrasts. Here, we present a panoramic PA-US imaging platform that integrates multispectral PA computed tomography (PACT) along with reflection-mode and transmission-mode US imaging through a single shared full-ring ultrasound array. We employ an ultrafast planewave transmission scheme in reflection-mode US for power Doppler (PWD) imaging and ultrasound localization microscopy (ULM). Additionally, we use the transmission-mode US to reconstruct a spatially resolved speed of sound (SoS) map that corrects both PA and US reconstruction. Such correction sharpens the resolution of PACT, suppresses the artifacts of PWD, and improves microbubble localization of ULM. Elevational scanning further enables whole-body volumetric imaging with co-registered PA and US contrasts. The integrated system maps photoswitchable DrBphP1-expressing tumors alongside their blood perfusion and oxygenation environment. Applying the platform to monitor unilateral renal ischemia-reperfusion injury, we report that microvascular perfusion and renal oxygenation recover at different rates. Collectively, we demonstrate that the integrated PA-US imaging platform provides a unified framework for multiparametric study of anatomy, perfusion, microvascular flow, oxygenation, and molecular activities.
Related
- Contrast‐enhanced ultrasound and photoacoustic imaging to assess early and late renal ischemia‐reperfusion injuries in male mice
- Ultrahigh-resolution ophthalmic optical coherence tomography
- Physics-aware resolution enhancement of soft X-ray tomography with measurement-supervised learning
- Contact-Free Nuclear and Cytoplasmic Mechanophenotyping by Amplitude-Modulated Ultrasound and Phase-Locked Optical Diffraction Tomography: Computational Feasibility and Initial Experimental Readouts
- Large Field, High Resolution Full-Field Optical Coherence Tomography: A Pre-clinical Study of Human Breast Tissue and Cancer Assessment
BibTeX
@misc{yao2026wholebody,
title = {{Whole-body Super-resolution Functional and Molecular Imaging with Panoramic Photoacoustic-Ultrasound Tomography}},
author = {R. Yao and I. Husain and J. Luo and H. Huo and X. Cai and N. Wang and T. Vu and J. Li and Y. Xu and L. Menozzi and J. J. Yang and M. Lowerison and X. Luo and P. Song and J. Yao},
howpublished = {bioRxiv},
year = {2026},
month = aug,
doi = {10.64898/2026.08.28.747673},
url = {https://www.biorxiv.org/content/10.64898/2026.08.28.747673v1},
note = {CC BY-NC},
}
RIS
TY - UNPB
TI - Whole-body Super-resolution Functional and Molecular Imaging with Panoramic Photoacoustic-Ultrasound Tomography
AU - R. Yao
AU - I. Husain
AU - J. Luo
AU - H. Huo
AU - X. Cai
AU - N. Wang
AU - T. Vu
AU - J. Li
AU - Y. Xu
AU - L. Menozzi
AU - J. J. Yang
AU - M. Lowerison
AU - X. Luo
AU - P. Song
AU - J. Yao
PY - 2026
DA - 2026/08/28
PB - bioRxiv
DO - 10.64898/2026.08.28.747673
UR - https://www.biorxiv.org/content/10.64898/2026.08.28.747673v1
AB - Photoacoustic (PA) and ultrasound (US) imaging provide complementary molecular, functional, and anatomical contrasts. Here, we present a panoramic PA-US imaging platform that integrates multispectral PA computed tomography (PACT) along with reflection-mode and transmission-mode US imaging through a single shared full-ring ultrasound array. We employ an ultrafast planewave transmission scheme in reflection-mode US for power Doppler (PWD) imaging and ultrasound localization microscopy (ULM). Additionally, we use the transmission-mode US to reconstruct a spatially resolved speed of sound (SoS) map that corrects both PA and US reconstruction. Such correction sharpens the resolution of PACT, suppresses the artifacts of PWD, and improves microbubble localization of ULM. Elevational scanning further enables whole-body volumetric imaging with co-registered PA and US contrasts. The integrated system maps photoswitchable DrBphP1-expressing tumors alongside their blood perfusion and oxygenation environment. Applying the platform to monitor unilateral renal ischemia-reperfusion injury, we report that microvascular perfusion and renal oxygenation recover at different rates. Collectively, we demonstrate that the integrated PA-US imaging platform provides a unified framework for multiparametric study of anatomy, perfusion, microvascular flow, oxygenation, and molecular activities.
ER -