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Peer-reviewedCC BYJournal of clinical medicine

Long-Term Follow-Up After Testicular Tissue Cryopreservation in Boys Facing Gonadotoxic Therapies: Safety, Pubertal Development, Reproductive Outcomes, and Clinical Implications

  • Lucia Paolini1
  • Vittorio Ferrari2
  • Umberto Cattini2
  • Elisa Bortolamedi2
  • Marcello Lanari2,3
  • Federico Baronio2,3
  1. 1Specialty School of Pediatrics, Alma Mater Studiorum, University of Bologna, 40138 Bologna, Italy
  2. 2Pediatric Unit, IRCCS Azienda Ospedaliero-Universitaria di Bologna, 40139 Bologna, Italy
  3. 3Department of Medical and Surgical Sciences, Alma Mater Studiorum, University of Bologna, 40126 Bologna, Italy
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Abstract

Testicular tissue cryopreservation is increasingly offered to boys facing gonadotoxic therapies. Yet, its long-term clinical significance depends not only on future fertility-restoration technologies but also on how these patients evolve. This narrative review aims to synthesizes the available follow-up literature on boys who underwent testicular tissue cryopreservation, focusing on procedural safety, testicular growth, pubertal progression, endocrine function, semen outcomes in patients reaching adulthood, and the emerging question of whether cryopreserved tissue may ultimately be needed in all cases. We will compare follow-up strategies across centers, highlight the lack of standardized endpoints, and discuss how survivorship data may refine counseling, re-consent, and future management of cryopreserved tissue. The aim is to provide a clinically oriented overview of what current longitudinal cohorts can already tell us, while identifying the key evidence gaps that still limit harmonized follow-up protocols.

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Cite

BibTeXRIS
BibTeX
@article{paolini2026longterm,
  title = {{Long-Term Follow-Up After Testicular Tissue Cryopreservation in Boys Facing Gonadotoxic Therapies: Safety, Pubertal Development, Reproductive Outcomes, and Clinical Implications}},
  author = {Lucia Paolini and Vittorio Ferrari and Umberto Cattini and Elisa Bortolamedi and Marcello Lanari and Federico Baronio},
  journal = {Journal of clinical medicine},
  year = {2026},
  month = oct,
  doi = {10.3390/jcm15197759},
  url = {https://europepmc.org/article/PMC/PMC13665890},
  note = {CC BY},
}
RIS
TY  - JOUR
TI  - Long-Term Follow-Up After Testicular Tissue Cryopreservation in Boys Facing Gonadotoxic Therapies: Safety, Pubertal Development, Reproductive Outcomes, and Clinical Implications
AU  - Lucia Paolini
AU  - Vittorio Ferrari
AU  - Umberto Cattini
AU  - Elisa Bortolamedi
AU  - Marcello Lanari
AU  - Federico Baronio
PY  - 2026
DA  - 2026/10/01
JO  - Journal of clinical medicine
DO  - 10.3390/jcm15197759
UR  - https://europepmc.org/article/PMC/PMC13665890
AB  - Testicular tissue cryopreservation is increasingly offered to boys facing gonadotoxic therapies. Yet, its long-term clinical significance depends not only on future fertility-restoration technologies but also on how these patients evolve. This narrative review aims to synthesizes the available follow-up literature on boys who underwent testicular tissue cryopreservation, focusing on procedural safety, testicular growth, pubertal progression, endocrine function, semen outcomes in patients reaching adulthood, and the emerging question of whether cryopreserved tissue may ultimately be needed in all cases. We will compare follow-up strategies across centers, highlight the lack of standardized endpoints, and discuss how survivorship data may refine counseling, re-consent, and future management of cryopreserved tissue. The aim is to provide a clinically oriented overview of what current longitudinal cohorts can already tell us, while identifying the key evidence gaps that still limit harmonized follow-up protocols.
SN  - 2077-0383
ER  -