Skip to content
DraconiticResearch
Search Sources API
203,234 studies31 sourcesUpdated 30 min ago
Peer-reviewedCC BY 4.0International Journal of Molecular Sciences

A Novel 26-bp Insertion in the PpMYB10.1 Promoter Is Associated with Anthocyanin Deficiency in Peach (Prunus persica) Fruit Skin

  • Zhenhua Lu1,2,3
  • Lei Pan1,3
  • Zhenyu Huang1,2
  • Liang Niu1,3
  • Guochao Cui1
  • Wenfang Zeng1
  • Lifeng Liu1
  • Suke Wang1
  • Luwei Wang1
  • Hao Wang1
  • Zhiqiang Wang1
  1. 1Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, China
  2. 2Key Laboratory of Genetic Resource Evaluation and Application of Horticultural Crops (Fruit), Ministry of Agriculture and Rural Affairs, Zhengzhou 450009, China
  3. 3Henan Joint International Research Laboratory of Fruit Tree Genomics, Zhengzhou 450009, China
Open at sourceDOICite

Abstract

Fruit skin color is a decisive agronomic trait governing the commercial value of peaches; although PpMYB10.1 is known to regulate anthocyanin accumulation in peach, the specific promoter structural variant that silences PpMYB10.1 expression in non-red skin types has not been identified and functionally validated. Here, using a first backcross (BC1) population, we integrated bulked segregant analysis sequencing (BSA-seq), transcriptome profiling, and whole-genome resequencing to map the causal locus to a 453.083-kb interval on chromosome 3 harboring the R2R3-MYB transcription factor PpMYB10.1. Transient overexpression and virus-induced gene silencing (VIGS), together with stable tobacco transformation, confirmed that PpMYB10.1 regulates the non-red skin phenotype in this population. Sequence comparison revealed a 26-bp insertion in the PpMYB10.1 promoter of non-red fruit; reporter assays demonstrated that this insertion strongly reduces promoter activity and silences PpMYB10.1 expression. A co-dominant InDel marker co-segregated with the skin color phenotype in all tested accessions and populations. Our findings suggest a promoter structural variant is associated with the molecular switch controlling anthocyanin accumulation in peach skin and provide a practical tool for marker-assisted selection.

Related

  1. Integrated multi-omics analysis of flavonoid metabolism in soybean seeds of different colours reveals candidate regulatory networks associated with anthocyanin and isoflavone accumulation
    Yan Qin, Wenjie Chen, Yuan Chen +10
    Food Chemistry: Molecular SciencesPeer-reviewedCC BY-NC 4.0Full textMolecular biology & gene regulationPMC
  2. Dynamic merging of enhancer- and promoter-associated assemblies is coupled to transcriptional bursting
    Q. Lin, B. Wang, Y. Liu +9
    bioRxivPreprintAll rights reservedFull textMolecular biology & gene regulationbioRxiv
  3. Single-Molecule Nanopore Sequencing of the TERT Core Promoter CpG Island Reveals PQS-Associated Strand Asymmetry of De Novo Methylation and Its Modulation by Photo-Oxidation
    Alexander Sergeev, Adelya Genatullina, Viktoria Snyga +4
    International Journal of Molecular SciencesPeer-reviewedCC BY 4.0Full textMolecular biology & gene regulationCrossrefEurope PMC
  4. Disruption of SWI/SNF ATPases in the mouse liver impairs diurnal gene expression associated with specific promoter features.
    Nicolaj I Toft, Victor E Goitea, Stine M Præstholm +5
    Nature communicationsPeer-reviewedCC BY-NC-NDFull textMolecular biology & gene regulationEurope PMC
  5. Ceruloplasmin deficiency promotes osteoclast cuproptosis-like cell death and is associated with higher bone mass in OVX mice with coordinated activation of AMPK/p53 signaling
    Guiping Chen, Feng Yan, Qiangqiang Xiong +2
    Scientific ReportsPeer-reviewedCC BY 4.0Full textMolecular biology & gene regulationCrossref

Cite

BibTeXRIS
BibTeX
@article{lu2026novel,
  title = {{A Novel 26-bp Insertion in the PpMYB10.1 Promoter Is Associated with Anthocyanin Deficiency in Peach (Prunus persica) Fruit Skin}},
  author = {Zhenhua Lu and Lei Pan and Zhenyu Huang and Liang Niu and Guochao Cui and Wenfang Zeng and Lifeng Liu and Suke Wang and Luwei Wang and Hao Wang and Zhiqiang Wang},
  journal = {International Journal of Molecular Sciences},
  year = {2026},
  month = nov,
  doi = {10.3390/ijms272110027},
  url = {https://www.mdpi.com/1422-0067/27/21/10027},
  note = {CC BY 4.0},
}
RIS
TY  - JOUR
TI  - A Novel 26-bp Insertion in the PpMYB10.1 Promoter Is Associated with Anthocyanin Deficiency in Peach (Prunus persica) Fruit Skin
AU  - Zhenhua Lu
AU  - Lei Pan
AU  - Zhenyu Huang
AU  - Liang Niu
AU  - Guochao Cui
AU  - Wenfang Zeng
AU  - Lifeng Liu
AU  - Suke Wang
AU  - Luwei Wang
AU  - Hao Wang
AU  - Zhiqiang Wang
PY  - 2026
DA  - 2026/11/09
JO  - International Journal of Molecular Sciences
DO  - 10.3390/ijms272110027
UR  - https://www.mdpi.com/1422-0067/27/21/10027
AB  - Fruit skin color is a decisive agronomic trait governing the commercial value of peaches; although PpMYB10.1 is known to regulate anthocyanin accumulation in peach, the specific promoter structural variant that silences PpMYB10.1 expression in non-red skin types has not been identified and functionally validated. Here, using a first backcross (BC1) population, we integrated bulked segregant analysis sequencing (BSA-seq), transcriptome profiling, and whole-genome resequencing to map the causal locus to a 453.083-kb interval on chromosome 3 harboring the R2R3-MYB transcription factor PpMYB10.1. Transient overexpression and virus-induced gene silencing (VIGS), together with stable tobacco transformation, confirmed that PpMYB10.1 regulates the non-red skin phenotype in this population. Sequence comparison revealed a 26-bp insertion in the PpMYB10.1 promoter of non-red fruit; reporter assays demonstrated that this insertion strongly reduces promoter activity and silences PpMYB10.1 expression. A co-dominant InDel marker co-segregated with the skin color phenotype in all tested accessions and populations. Our findings suggest a promoter structural variant is associated with the molecular switch controlling anthocyanin accumulation in peach skin and provide a practical tool for marker-assisted selection.
SN  - 1422-0067
ER  -