Subtortins A-C, Pyran-3-one-Containing Malabaricanes Isolated from Cortinarius subtortus
Abstract
Cortinarius (webcaps) is the most diversified genus of mushrooms, yet mycochemical studies so far seem to have mostly investigated toxic or colorful species. The aim of our project was to leverage HPLC-DAD-HRMS/MS data from our local fungal extract library to target and isolate compounds found specifically in Cortinarius extracts. The selection and fractionation of an ethyl acetate extract obtained from sporophores of Cortinarius subtortus led to the isolation and structure elucidation of three new uncommon triterpenes named subtortins A−C (1−3). These compounds belong to the malabaricane triterpenoid subclass, arising from an incomplete cyclization of squalene, and feature an unprecedented terminal pyran-3-one moiety. To date, only very few malabaricanes of fungal origin have been reported, all from Cortinarius, suggesting a potential chemotaxonomic significance. Structure elucidation and absolute configuration assignment of subtortins A−C (1−3) were established by a combination of experimental spectroscopies (NMR and ECD) and computational approaches based on GIAO NMR and TDDFT-ECD. Antimicrobial assays against the pathogenic models Staphylococcus aureus, Escherichia coli, and Candida albicans showed no activity, while the possible biosynthesis and ecological role of these metabolites are discussed.
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BibTeX
@article{ozga2026subtortins,
title = {{Subtortins A-C, Pyran-3-one-Containing Malabaricanes Isolated from Cortinarius subtortus}},
author = {Théo Ozga and Mehdi A Beniddir and Jean-Michel Bellanger and Arnaud Marvilliers and Emmanuelle Girard-Valenciennes and Aurélie Urbain},
journal = {Journal of Natural Products},
year = {2026},
month = oct,
doi = {10.1021/acs.jnatprod.6c00850},
url = {https://univoak.hal.science/hal-05780142v1},
note = {CC BY-NC 4.0},
}
RIS
TY - JOUR
TI - Subtortins A-C, Pyran-3-one-Containing Malabaricanes Isolated from Cortinarius subtortus
AU - Théo Ozga
AU - Mehdi A Beniddir
AU - Jean-Michel Bellanger
AU - Arnaud Marvilliers
AU - Emmanuelle Girard-Valenciennes
AU - Aurélie Urbain
PY - 2026
DA - 2026/10/03
JO - Journal of Natural Products
DO - 10.1021/acs.jnatprod.6c00850
UR - https://univoak.hal.science/hal-05780142v1
AB - Cortinarius (webcaps) is the most diversified genus of mushrooms, yet mycochemical studies so far seem to have mostly investigated toxic or colorful species. The aim of our project was to leverage HPLC-DAD-HRMS/MS data from our local fungal extract library to target and isolate compounds found specifically in Cortinarius extracts. The selection and fractionation of an ethyl acetate extract obtained from sporophores of Cortinarius subtortus led to the isolation and structure elucidation of three new uncommon triterpenes named subtortins A−C (1−3). These compounds belong to the malabaricane triterpenoid subclass, arising from an incomplete cyclization of squalene, and feature an unprecedented terminal pyran-3-one moiety. To date, only very few malabaricanes of fungal origin have been reported, all from Cortinarius, suggesting a potential chemotaxonomic significance. Structure elucidation and absolute configuration assignment of subtortins A−C (1−3) were established by a combination of experimental spectroscopies (NMR and ECD) and computational approaches based on GIAO NMR and TDDFT-ECD. Antimicrobial assays against the pathogenic models Staphylococcus aureus, Escherichia coli, and Candida albicans showed no activity, while the possible biosynthesis and ecological role of these metabolites are discussed.
SN - 0163-3864
SN - 1520-6025
ER -