Statistical analysis provides new insights into patterns and mechanisms of the WANCY hotspot on vertebrate mitochondrial genomes
- 1Imperial college london / London Natural History Museum
Abstract
The vertebrate mitochondrial genomes are commonly composed of 37 genes, and the order of these genes act as useful indicator of phylogenetic relationships. Tandem duplication and random loss (TDRL) is considered to be one of the most important mechanisms leading to mitochondrial gene order rearrangement in vertebrates. The WANCY region has been widely recognized as a TDRL hotspot. In the past, the lack of data limited the in-depth exploration of rearrangement patterns and mechanisms in hotspots. Through statistical analysis of a large amount of available NCBI sequencing data, combined with phylogenetic and bioinformatics methods, we obtained statistically significant data from the four classes of Amphibians, Reptiles, Mammalia and Osteichthyes. These data demonstrate three new convergent rearrangements in the WANCY region of vertebrates, which suggests that the frequency of convergent rearrangements in hotspots is higher than previously thought. We also compared the frequency of TDRL rearrangement in the WANCY region with two other similar small gene clusters (the IQM region and the HSL region). The results to a certain extent support the hypothesis that the WANCY region may be a mechanism hotspot. However, more molecular evidence and larger data volumes are needed to confirm the existence of mechanisms that cause rearrangement hotspots.
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BibTeX
@misc{xu2026statistical,
title = {{Statistical analysis provides new insights into patterns and mechanisms of the WANCY hotspot on vertebrate mitochondrial genomes}},
author = {C. Xu and M. Wilkinson},
howpublished = {bioRxiv},
year = {2026},
month = oct,
doi = {10.64898/2026.10.16.759661},
url = {https://www.biorxiv.org/content/10.64898/2026.10.16.759661v1},
note = {CC BY},
}
RIS
TY - UNPB
TI - Statistical analysis provides new insights into patterns and mechanisms of the WANCY hotspot on vertebrate mitochondrial genomes
AU - C. Xu
AU - M. Wilkinson
PY - 2026
DA - 2026/10/16
PB - bioRxiv
DO - 10.64898/2026.10.16.759661
UR - https://www.biorxiv.org/content/10.64898/2026.10.16.759661v1
AB - The vertebrate mitochondrial genomes are commonly composed of 37 genes, and the order of these genes act as useful indicator of phylogenetic relationships. Tandem duplication and random loss (TDRL) is considered to be one of the most important mechanisms leading to mitochondrial gene order rearrangement in vertebrates. The WANCY region has been widely recognized as a TDRL hotspot. In the past, the lack of data limited the in-depth exploration of rearrangement patterns and mechanisms in hotspots. Through statistical analysis of a large amount of available NCBI sequencing data, combined with phylogenetic and bioinformatics methods, we obtained statistically significant data from the four classes of Amphibians, Reptiles, Mammalia and Osteichthyes. These data demonstrate three new convergent rearrangements in the WANCY region of vertebrates, which suggests that the frequency of convergent rearrangements in hotspots is higher than previously thought. We also compared the frequency of TDRL rearrangement in the WANCY region with two other similar small gene clusters (the IQM region and the HSL region). The results to a certain extent support the hypothesis that the WANCY region may be a mechanism hotspot. However, more molecular evidence and larger data volumes are needed to confirm the existence of mechanisms that cause rearrangement hotspots.
ER -