A Chemically Characterized Extract of Centipeda minima Attenuates Inflammation in Macrophages and Alleviates Osteoarthritis Progression in Rats
- 1College of Korean Medicine, Gachon University, 1342, Seongnamdae-ro, Sujeong-gu, Seongnam 13120, Republic of Korea
- 2Institute of Natural Products, Korea Institute of Science and Technology, 679, Saimdang-ro, Gangneung 25451, Republic of Korea
- 3School of Pharmacy, Sungkyunkwan University, Suwon 16419, Republic of Korea
Abstract
Centipeda minima, an edible herb with medicinal properties, contains bioactive sesquiterpene lactones with anti-inflammatory potential, but its nutraceutical application is limited by insufficient standardization. This study evaluated the anti-inflammatory mechanisms of a chemically characterized bioactive-rich extract from C. minima (CMXP) and its four isolated active compounds. In LPS-stimulated RAW 264.7 macrophages, CMXP and the four isolated sesquiterpene lactones inhibited nitric oxide (NO) production in a concentration-dependent manner. These inhibitory effects were evident within concentration ranges that did not markedly affect cell viability, whereas reduced cell viability was observed at the highest tested concentrations of CMXP (10 μg/mL), arnicolide C (10 μM), and brevilin A (10 μM). CMXP markedly suppressed iNOS and COX-2 expression, reducing ERK and JNK phosphorylation. In the MIA-induced osteoarthritis rat model, CMXP improved hind limb weight-bearing function, decreased serum IL-6 and cartilage MMP-3 expression, and attenuated subchondral bone loss, as confirmed by micro-computed tomography. CMXP demonstrated anti-inflammatory effects through MAPK pathway modulation and improved OA-associated functional and structural changes. The chemical characterization of CMXP provides a basis for further investigation of its potential application in OA.
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BibTeX
@article{lee2026chemically,
title = {{A Chemically Characterized Extract of Centipeda minima Attenuates Inflammation in Macrophages and Alleviates Osteoarthritis Progression in Rats}},
author = {Myong Jin Lee and Young-Joo Kim and Byoung Ha Kim and Shin Jung Park and Hea Sun Chun and Ki Sung Kang},
journal = {Molecules},
year = {2026},
month = oct,
doi = {10.3390/molecules31203639},
url = {https://www.mdpi.com/1420-3049/31/20/3639},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - A Chemically Characterized Extract of Centipeda minima Attenuates Inflammation in Macrophages and Alleviates Osteoarthritis Progression in Rats
AU - Myong Jin Lee
AU - Young-Joo Kim
AU - Byoung Ha Kim
AU - Shin Jung Park
AU - Hea Sun Chun
AU - Ki Sung Kang
PY - 2026
DA - 2026/10/01
JO - Molecules
DO - 10.3390/molecules31203639
UR - https://www.mdpi.com/1420-3049/31/20/3639
AB - Centipeda minima, an edible herb with medicinal properties, contains bioactive sesquiterpene lactones with anti-inflammatory potential, but its nutraceutical application is limited by insufficient standardization. This study evaluated the anti-inflammatory mechanisms of a chemically characterized bioactive-rich extract from C. minima (CMXP) and its four isolated active compounds. In LPS-stimulated RAW 264.7 macrophages, CMXP and the four isolated sesquiterpene lactones inhibited nitric oxide (NO) production in a concentration-dependent manner. These inhibitory effects were evident within concentration ranges that did not markedly affect cell viability, whereas reduced cell viability was observed at the highest tested concentrations of CMXP (10 μg/mL), arnicolide C (10 μM), and brevilin A (10 μM). CMXP markedly suppressed iNOS and COX-2 expression, reducing ERK and JNK phosphorylation. In the MIA-induced osteoarthritis rat model, CMXP improved hind limb weight-bearing function, decreased serum IL-6 and cartilage MMP-3 expression, and attenuated subchondral bone loss, as confirmed by micro-computed tomography. CMXP demonstrated anti-inflammatory effects through MAPK pathway modulation and improved OA-associated functional and structural changes. The chemical characterization of CMXP provides a basis for further investigation of its potential application in OA.
SN - 1420-3049
ER -