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Peer-reviewedCC BY 4.0Biology Open

Dynamin-2 promotes Atg9A retrieval from phagophores during autophagy

  • Andrew Caliri1
  • Alejandro Martorell-Riera1
  • Panagiota Kolitsida1
  • Akash Saha1
  • Cinta Iriondo-Martinez1
  • Samuel Itskanov1
  • Janos Steffen2
  • Carla M. Koehler2
  • Alexander M. van der Bliek1
  1. 1Department of Biological Chemistry, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA
  2. 2Department of Chemistry and Biochemistry, UCLA, Los Angeles, CA 90095, USA
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Abstract

Autophagy involves the rapid growth of phagophores through membrane addition. This growth is triggered by vesicles containing the Atg9A protein. However, Atg9A is not incorporated into mature autophagosomes. We now demonstrate that Dynamin-2 (Dnm2) colocalizes with the BAR domain protein endophilin B1 (EndoB1/Bif-1/SH3GLB1) and other autophagy proteins when autophagy is induced. Our data suggest that Atg9A is retrieved from phagophores via fission, with help from Dnm2. Blocking Atg9A recycling, by mutating Dnm2, using RNA interference, or applying chemical inhibitors, causes Atg9A to remain in autophagosomes and be degraded during autophagy. Overall, these findings provide new insights into the roles of Dnm2 in autophagy.

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Cite

BibTeXRIS
BibTeX
@article{caliri2026dynamin2,
  title = {{Dynamin-2 promotes Atg9A retrieval from phagophores during autophagy}},
  author = {Andrew Caliri and Alejandro Martorell-Riera and Panagiota Kolitsida and Akash Saha and Cinta Iriondo-Martinez and Samuel Itskanov and Janos Steffen and Carla M. Koehler and Alexander M. van der Bliek},
  journal = {Biology Open},
  year = {2026},
  month = oct,
  doi = {10.1242/bio.062632},
  url = {https://doi.org/10.1242/bio.062632},
  note = {CC BY 4.0},
}
RIS
TY  - JOUR
TI  - Dynamin-2 promotes Atg9A retrieval from phagophores during autophagy
AU  - Andrew Caliri
AU  - Alejandro Martorell-Riera
AU  - Panagiota Kolitsida
AU  - Akash Saha
AU  - Cinta Iriondo-Martinez
AU  - Samuel Itskanov
AU  - Janos Steffen
AU  - Carla M. Koehler
AU  - Alexander M. van der Bliek
PY  - 2026
DA  - 2026/10/16
JO  - Biology Open
DO  - 10.1242/bio.062632
UR  - https://doi.org/10.1242/bio.062632
AB  - Autophagy involves the rapid growth of phagophores through membrane addition. This growth is triggered by vesicles containing the Atg9A protein. However, Atg9A is not incorporated into mature autophagosomes. We now demonstrate that Dynamin-2 (Dnm2) colocalizes with the BAR domain protein endophilin B1 (EndoB1/Bif-1/SH3GLB1) and other autophagy proteins when autophagy is induced. Our data suggest that Atg9A is retrieved from phagophores via fission, with help from Dnm2. Blocking Atg9A recycling, by mutating Dnm2, using RNA interference, or applying chemical inhibitors, causes Atg9A to remain in autophagosomes and be degraded during autophagy. Overall, these findings provide new insights into the roles of Dnm2 in autophagy.
SN  - 2046-6390
ER  -