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Peer-reviewedCC BYCellular and molecular life sciences : CMLS

Deciphering the heterogeneity of chicken gonadal primordial germ cells via single-cell RNA sequencing.

  • Xiaomin Wang1
  • Shenghu Zhang2
  • Kaipeng Wang2,3
  • Qianru Li2
  • Yihang Yang2
  • Xingnan Huang2
  • Zhuangfei Wang2
  • Mengmeng Guo2
  • Bo Chen2
  • Zheting Zhang2
  • Bo Wang4,5
  • Duanqing Pei6,7
1 more authors
  • Chengchen Zhao8,9,10
10 affiliations
  1. 1Laboratory of Cell Fate Control, School of Life Sciences, Westlake University, Hangzhou, China. [email protected].
  2. 2Laboratory of Cell Fate Control, School of Life Sciences, Westlake University, Hangzhou, China.
  3. 3Fudan University, Shanghai, China.
  4. 4Zhejiang University of Science and Technology School of Information and Electronic Engineering, Hangzhou, China. [email protected].
  5. 5Zhejiang Key Laboratory of Biomedical Intelligent Computing Technology, Hangzhou, China. [email protected].
  6. 6Laboratory of Cell Fate Control, School of Life Sciences, Westlake University, Hangzhou, China. [email protected].
  7. 7Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, Zhejiang, China. [email protected].
  8. 8Laboratory of Cell Fate Control, School of Life Sciences, Westlake University, Hangzhou, China. [email protected].
  9. 9Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, Zhejiang, China. [email protected].
  10. 10Zhejiang Key Laboratory of Biomedical Intelligent Computing Technology, Hangzhou, China. [email protected].
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Abstract

Chicken primordial germ cells (PGCs) are valuable for breeding, germplasm preservation, and genetic modifications. Here, using single-cell transcriptome analysis, we found that three transcriptionally distinct PGC subtypes were exist in chicken embryonic gonads PGCs. We further demonstrated that in vitro-cultured PGCs could integrate into recipient gonads via blood vessel injection. Additionally, the gene-modified PGCs could be re-established and integrated into recipient gonads after the PGCs were cultured for long-term use by a second blood-vessel injection. Together, these results provide evidence for substantial heterogeneity among chicken gonadal PGCs, which were previously considered a homogeneous cell population. This diversity could be leveraged to establish and expand PGCs for long-term in vitro culture from differentiated gonads.

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Cite

BibTeXRIS
BibTeX
@article{wang2026deciphering,
  title = {{Deciphering the heterogeneity of chicken gonadal primordial germ cells via single-cell RNA sequencing.}},
  author = {Xiaomin Wang and Shenghu Zhang and Kaipeng Wang and Qianru Li and Yihang Yang and Xingnan Huang and Zhuangfei Wang and Mengmeng Guo and Bo Chen and Zheting Zhang and Bo Wang and Duanqing Pei and Chengchen Zhao},
  journal = {Cellular and molecular life sciences : CMLS},
  year = {2026},
  month = jul,
  doi = {10.1007/s00018-026-06331-w},
  url = {https://europepmc.org/article/MED/42897262},
  note = {CC BY},
}
RIS
TY  - JOUR
TI  - Deciphering the heterogeneity of chicken gonadal primordial germ cells via single-cell RNA sequencing.
AU  - Xiaomin Wang
AU  - Shenghu Zhang
AU  - Kaipeng Wang
AU  - Qianru Li
AU  - Yihang Yang
AU  - Xingnan Huang
AU  - Zhuangfei Wang
AU  - Mengmeng Guo
AU  - Bo Chen
AU  - Zheting Zhang
AU  - Bo Wang
AU  - Duanqing Pei
AU  - Chengchen Zhao
PY  - 2026
DA  - 2026/07/01
JO  - Cellular and molecular life sciences : CMLS
DO  - 10.1007/s00018-026-06331-w
UR  - https://europepmc.org/article/MED/42897262
AB  - Chicken primordial germ cells (PGCs) are valuable for breeding, germplasm preservation, and genetic modifications. Here, using single-cell transcriptome analysis, we found that three transcriptionally distinct PGC subtypes were exist in chicken embryonic gonads PGCs. We further demonstrated that in vitro-cultured PGCs could integrate into recipient gonads via blood vessel injection. Additionally, the gene-modified PGCs could be re-established and integrated into recipient gonads after the PGCs were cultured for long-term use by a second blood-vessel injection. Together, these results provide evidence for substantial heterogeneity among chicken gonadal PGCs, which were previously considered a homogeneous cell population. This diversity could be leveraged to establish and expand PGCs for long-term in vitro culture from differentiated gonads.
SN  - 1420-682X
SN  - 1420-9071
ER  -