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Peer-reviewedCC BY 4.0Scientific Reports

pCORE, a set of open modular plasmids compatible with golden gate assembly

  • Louisa Gonzalez Somermeyer1
  • Maxim M. Perfilov2,3
  • Feng Gao4,5
  • Dmitry A. Gorbachev2,3
  • Nikita V. Sytov2
  • Victoria Kovalenko3
  • Macià Sureda-Vives4,5
  • Aubin Fleiss4,5
  • Pilar Baldominos1
  • Ilia V. Yampolsky2
  • Fyodor A. Kondrashov1,6
  • Alexander S. Mishin2
1 more authors
  • Karen S. Sarkisyan3,4,5
6 affiliations
  1. 1Institute of Science and Technology Austria, Klosterneuburg, Austria
  2. 2Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia
  3. 3Cloning Facility, Moscow, Russia
  4. 4MRC Laboratory of Medical Sciences, London, UK
  5. 5Institute of Clinical Sciences, Faculty of Medicine, Imperial College London, London, UK
  6. 6Evolutionary and Synthetic Biology Unit, Okinawa Institute of Science and Technology Graduate University, Okinawa, Japan
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Abstract

Modular DNA assembly based Golden Gate cloning (MoClo) is a widely used framework to assemble genetic constructs. A number of MoClo “destination vectors” have been developed, however, available options are still limited with regards to copy number and antibiotic resistance in bacteria, vector compactness, and industry researchers’ access to materials. Here, we assembled combinations of five resistance cassettes, four origins of replication and two colour markers to develop a collection of compact MoClo-compatible pCORE destination vectors. pCORE vectors support MoClo Level 1 and Level 2 assembly, and can serve as starting points for developing vectors for other hosts. We tested the performance of our vector collection in Escherichia coli, Agrobacterium tumefaciens and Saccharomyces cerevisiae. pCORE vectors are available to academia and industry under the terms of OpenMTA licence.

Supplementary Information: The online version contains supplementary material available at https://doi.org/10.1038/s41598-026-66483-4.

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Cite

BibTeXRIS
BibTeX
@article{somermeyer2026pcore,
  title = {{pCORE, a set of open modular plasmids compatible with golden gate assembly}},
  author = {Louisa Gonzalez Somermeyer and Maxim M. Perfilov and Feng Gao and Dmitry A. Gorbachev and Nikita V. Sytov and Victoria Kovalenko and Macià Sureda-Vives and Aubin Fleiss and Pilar Baldominos and Ilia V. Yampolsky and Fyodor A. Kondrashov and Alexander S. Mishin and Karen S. Sarkisyan},
  journal = {Scientific Reports},
  year = {2026},
  month = aug,
  doi = {10.1038/s41598-026-66483-4},
  url = {https://doi.org/10.1038/s41598-026-66483-4},
  note = {CC BY 4.0},
}
RIS
TY  - JOUR
TI  - pCORE, a set of open modular plasmids compatible with golden gate assembly
AU  - Louisa Gonzalez Somermeyer
AU  - Maxim M. Perfilov
AU  - Feng Gao
AU  - Dmitry A. Gorbachev
AU  - Nikita V. Sytov
AU  - Victoria Kovalenko
AU  - Macià Sureda-Vives
AU  - Aubin Fleiss
AU  - Pilar Baldominos
AU  - Ilia V. Yampolsky
AU  - Fyodor A. Kondrashov
AU  - Alexander S. Mishin
AU  - Karen S. Sarkisyan
PY  - 2026
DA  - 2026/08/15
JO  - Scientific Reports
DO  - 10.1038/s41598-026-66483-4
UR  - https://doi.org/10.1038/s41598-026-66483-4
AB  - Modular DNA assembly based Golden Gate cloning (MoClo) is a widely used framework to assemble genetic constructs. A number of MoClo “destination vectors” have been developed, however, available options are still limited with regards to copy number and antibiotic resistance in bacteria, vector compactness, and industry researchers’ access to materials. Here, we assembled combinations of five resistance cassettes, four origins of replication and two colour markers to develop a collection of compact MoClo-compatible pCORE destination vectors. pCORE vectors support MoClo Level 1 and Level 2 assembly, and can serve as starting points for developing vectors for other hosts. We tested the performance of our vector collection in Escherichia coli, Agrobacterium tumefaciens and Saccharomyces cerevisiae. pCORE vectors are available to academia and industry under the terms of OpenMTA licence.  Supplementary Information: The online version contains supplementary material available at https://doi.org/10.1038/s41598-026-66483-4.
SN  - 2045-2322
ER  -