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Peer-reviewedCC BY 4.0Communications Biology

Extrinsic MMPs drive epithelial shape change via basal extracellular matrix disassembly in the Drosophila wing disc

  • Chigusa Hinata1
  • Hirotatsu Nakagawa1
  • Shigenori Nonaka2,3,4,5
  • Katsuya Nozaki1
  • Yoshikatsu Sato6
  • Shizue Ohsawa1
6 affiliations
  1. 1Division of Biological Science, Graduate School of Science, Nagoya University, Nagoya, Japan
  2. 2The Graduate University for Advanced Studies (SOKENDAI), Okazaki, Japan
  3. 3Optics and Imaging Facility, Trans-Scale Biology Center, National Institute for Basic Biology, Okazaki, Japan
  4. 4Laboratory for Spatiotemporal Regulations, National Institute for Basic Biology, Okazaki, Japan
  5. 5Spatiotemporal Regulations Group, Exploratory Research Center for Life and Living Systems, Okazaki, Japan
  6. 6Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University, Nagoya, Japan and Division of Biological Science, Graduate School of Science, Nagoya University, Nagoya, Japan
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Abstract

Epithelial morphogenesis generates complex tissue architectures with remarkable reproducibility, yet how tissue-scale epithelial curvature transitions are triggered in vivo remains poorly understood. Here, we show that epithelial morphogenesis during early pupal development of the Drosophila wing disc is regulated non-autonomously through spatially restricted remodeling of the basal extracellular matrix (ECM) by neighboring tissues. Live imaging analyses reveal a previously unrecognized morphogenetic event at the larval-to-pupal stage, in which the wing disc epithelium undergoes a concave-to-convex curvature inversion. This curvature inversion is preceded by local loss of Collagen IV from the basal ECM, particularly in the notum and hinge regions. Rather than being driven autonomously by the epithelium, the shape change depends on adjacent non-epithelial cell populations, including myoblasts and tracheal cells, whose dynamic behaviors are spatially and temporally associated with basal ECM disassembly. Mechanistically, systemic ecdysone signaling activates Mmp1 and Mmp2 in these neighboring tissues, leading to localized basal ECM disassembly that permissively enables epithelial self-organization. Together, our findings identify extrinsic ECM remodeling at tissue interfaces as a non-autonomous regulatory layer that spatially licenses tissue-scale epithelial curvature transitions during development.

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Cite

BibTeXRIS
BibTeX
@article{hinata2026extrinsic,
  title = {{Extrinsic MMPs drive epithelial shape change via basal extracellular matrix disassembly in the Drosophila wing disc}},
  author = {Chigusa Hinata and Hirotatsu Nakagawa and Shigenori Nonaka and Katsuya Nozaki and Yoshikatsu Sato and Shizue Ohsawa},
  journal = {Communications Biology},
  year = {2026},
  month = may,
  doi = {10.1038/s42003-026-10376-9},
  url = {https://doi.org/10.1038/s42003-026-10376-9},
  note = {CC BY 4.0},
}
RIS
TY  - JOUR
TI  - Extrinsic MMPs drive epithelial shape change via basal extracellular matrix disassembly in the Drosophila wing disc
AU  - Chigusa Hinata
AU  - Hirotatsu Nakagawa
AU  - Shigenori Nonaka
AU  - Katsuya Nozaki
AU  - Yoshikatsu Sato
AU  - Shizue Ohsawa
PY  - 2026
DA  - 2026/05/29
JO  - Communications Biology
DO  - 10.1038/s42003-026-10376-9
UR  - https://doi.org/10.1038/s42003-026-10376-9
AB  - Epithelial morphogenesis generates complex tissue architectures with remarkable reproducibility, yet how tissue-scale epithelial curvature transitions are triggered in vivo remains poorly understood. Here, we show that epithelial morphogenesis during early pupal development of the Drosophila wing disc is regulated non-autonomously through spatially restricted remodeling of the basal extracellular matrix (ECM) by neighboring tissues. Live imaging analyses reveal a previously unrecognized morphogenetic event at the larval-to-pupal stage, in which the wing disc epithelium undergoes a concave-to-convex curvature inversion. This curvature inversion is preceded by local loss of Collagen IV from the basal ECM, particularly in the notum and hinge regions. Rather than being driven autonomously by the epithelium, the shape change depends on adjacent non-epithelial cell populations, including myoblasts and tracheal cells, whose dynamic behaviors are spatially and temporally associated with basal ECM disassembly. Mechanistically, systemic ecdysone signaling activates Mmp1 and Mmp2 in these neighboring tissues, leading to localized basal ECM disassembly that permissively enables epithelial self-organization. Together, our findings identify extrinsic ECM remodeling at tissue interfaces as a non-autonomous regulatory layer that spatially licenses tissue-scale epithelial curvature transitions during development.
SN  - 2399-3642
ER  -