Genome-Wide Identification of Serine carboxypeptidase-like (SCPL) Genes in Rice and Their Expression in Response to Rhizoctonia solani
- 1Shanghai Key Laboratory of Agricultural Genetics and Breeding (21DZ2271900), Biotechnology Research Institute of Shanghai Academy of Agricultural Sciences, Shanghai 201106, China
- 2Shanghai Pudong New Area Agricultural Technology Extension Center, Shanghai 201201, China
Abstract
Serine carboxypeptidase-like (SCPL) proteins play important roles in plant secondary metabolism, growth, and stress responses. Several SCPL members have been reported to participate in the biosynthesis of antimicrobial compounds associated with plant immunity. However, systematic characterization of the SCPL gene family in rice and its potential involvement in sheath blight (ShB) responses remains largely unclear. In this study, 45 SCPL genes were identified using the IRGSP-1.0 reference assembly and Ensembl Plants release 54 annotation and designated as OsSCPL1–OsSCPL45. Phylogenetic analysis of SCPL proteins from rice, Arabidopsis, soybean, and maize classified these proteins into three groups. Gene structures, conserved motifs, chromosomal distribution, and promoter cis-elements were subsequently analyzed. Chromosomal mapping and collinearity analysis revealed that both tandem and segmental duplications contributed to the expansion of the OsSCPL gene family. Promoter analysis identified numerous cis-elements associated with stress and hormone signaling. Mock-controlled RT-qPCR results of OsSCPL genes in resistant rice genotype YSBR1 and susceptible rice genotype Lemont (LE) showed that 14 OsSCPL genes were significantly up- or downregulated following Rhizoctonia solani inoculation in at least one genotype. The segmentally duplicated gene pair OsSCPL4 was significantly upregulated in both genotypes, whereas OsSCPL25 was significantly upregulated in YSBR1 but did not reach significance in LE. This study provides an updated genomic framework for the SCPL gene family in rice and suggests potential roles for OsSCPL4 and OsSCPL25 in rice responses to R. solani, laying a foundation for further functional studies.
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BibTeX
@article{wang2026genomewide,
title = {{Genome-Wide Identification of Serine carboxypeptidase-like (SCPL) Genes in Rice and Their Expression in Response to Rhizoctonia solani}},
author = {Yu Wang and Shiji Feng and Chuan Chen and Jinlong Xue and Shuwei Zhang and Ting He and Guimei Guo and Longhua Zhou and Chenghong Liu and Zhiwei Chen},
journal = {International Journal of Molecular Sciences},
year = {2026},
month = nov,
doi = {10.3390/ijms27219850},
url = {https://www.mdpi.com/1422-0067/27/21/9850},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Genome-Wide Identification of Serine carboxypeptidase-like (SCPL) Genes in Rice and Their Expression in Response to Rhizoctonia solani
AU - Yu Wang
AU - Shiji Feng
AU - Chuan Chen
AU - Jinlong Xue
AU - Shuwei Zhang
AU - Ting He
AU - Guimei Guo
AU - Longhua Zhou
AU - Chenghong Liu
AU - Zhiwei Chen
PY - 2026
DA - 2026/11/03
JO - International Journal of Molecular Sciences
DO - 10.3390/ijms27219850
UR - https://www.mdpi.com/1422-0067/27/21/9850
AB - Serine carboxypeptidase-like (SCPL) proteins play important roles in plant secondary metabolism, growth, and stress responses. Several SCPL members have been reported to participate in the biosynthesis of antimicrobial compounds associated with plant immunity. However, systematic characterization of the SCPL gene family in rice and its potential involvement in sheath blight (ShB) responses remains largely unclear. In this study, 45 SCPL genes were identified using the IRGSP-1.0 reference assembly and Ensembl Plants release 54 annotation and designated as OsSCPL1–OsSCPL45. Phylogenetic analysis of SCPL proteins from rice, Arabidopsis, soybean, and maize classified these proteins into three groups. Gene structures, conserved motifs, chromosomal distribution, and promoter cis-elements were subsequently analyzed. Chromosomal mapping and collinearity analysis revealed that both tandem and segmental duplications contributed to the expansion of the OsSCPL gene family. Promoter analysis identified numerous cis-elements associated with stress and hormone signaling. Mock-controlled RT-qPCR results of OsSCPL genes in resistant rice genotype YSBR1 and susceptible rice genotype Lemont (LE) showed that 14 OsSCPL genes were significantly up- or downregulated following Rhizoctonia solani inoculation in at least one genotype. The segmentally duplicated gene pair OsSCPL4 was significantly upregulated in both genotypes, whereas OsSCPL25 was significantly upregulated in YSBR1 but did not reach significance in LE. This study provides an updated genomic framework for the SCPL gene family in rice and suggests potential roles for OsSCPL4 and OsSCPL25 in rice responses to R. solani, laying a foundation for further functional studies.
SN - 1422-0067
ER -