Biological process engineering by Pseudomonas sp. ABC1: a discovery-stage ecological chassis for open bioproduction
- 1Escuela de Química y Farmacia. Centro de Investigación en Recursos Naturales y Sustentabilidad (CIRENYS). Universidad Bernardo O’Higgins, Santiago, Chile
- 2Escuela de Medicina, Facultad de Ciencias de la Salud, Universidad del Alba, La Serena, Chile
Abstract
IntroductionConventional industrial biotechnology remains constrained by contamination control requirements and energy-intensive biomass separation. Next-generation industrial biotechnology (NGIB) seeks microbial platforms that retain useful production phenotypes under open cultivation while enabling simpler process configurations. Here, we evaluated Pseudomonas sp. ABC1, a fluorescent siderophore-producing bacterium, as a discovery-stage ecological chassis for open bioproduction.MethodsSeven-day open, non-sterile static cultivation was integrated with community profiling, microscopy, elemental analysis, peptidomics, proteomics, and comparative genomics.ResultsABC1 formed a cohesive floating pellicle associated with a non-axenic bacterial consortium that included Pseudomonas, Delftia, Flavobacterium, Acidovorax, and additional environmental genera. Genome-informed reconstruction of representative community members indicated distributed capacities for iron acquisition, oxidative-stress protection, adhesion, nutrient acquisition, and extracellular-matrix metabolism. LC-MS/MS recovered 1,518 de novo peptide sequences, including 1,478 non-redundant sequences, with recurrent N-hydroxy-cyclo-ornithine derivatives and other modified peptide forms. These signatures are consistent with metallophore-associated biosynthesis predicted from the extensive iron-acquisition machinery previously described for ABC1. Comparative genomics further showed that ABC1 lacks complete canonical Pel, Psl, alginate, and PNAG systems but contains two extended loci encoding complementary functions for non-canonical extracellular-polysaccharide assembly, including rfbDCAB precursor synthesis, multiple glycosyltransferases, candidate Wzx/Wzy-like proteins, and export-associated components. ABC1 also encodes an expanded nutrient-acquisition architecture and 29 cyclic-di-GMP-associated proteins. Calcium-enriched pellicle microdomains and extracellular adhesive proteins provide additional candidate structural features, although mineral identity and polymer composition remain unresolved.DiscussionCollectively, these data support ABC1 as a promising discovery-stage system in which ecological selection, extracellular-matrix organization, and biosynthetic diversification can be investigated under open cultivation. Quantitative validation of productivity, contamination resistance, biomass-recovery performance, safety, and scale-up remains necessary before industrial advantage can be established.
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BibTeX
@article{valenzuelaheredia2026biological,
title = {{Biological process engineering by Pseudomonas sp. ABC1: a discovery-stage ecological chassis for open bioproduction}},
author = {Daniel Valenzuela-Heredia and Sebastián Fuentes-Alburquenque and Carla Trigo and Carlos Henríquez-Castillo},
journal = {Frontiers in Bioengineering and Biotechnology},
year = {2026},
month = oct,
doi = {10.3389/fbioe.2026.1926795},
url = {https://doi.org/10.3389/fbioe.2026.1926795},
note = {CC BY},
}
RIS
TY - JOUR
TI - Biological process engineering by Pseudomonas sp. ABC1: a discovery-stage ecological chassis for open bioproduction
AU - Daniel Valenzuela-Heredia
AU - Sebastián Fuentes-Alburquenque
AU - Carla Trigo
AU - Carlos Henríquez-Castillo
PY - 2026
DA - 2026/10/26
JO - Frontiers in Bioengineering and Biotechnology
DO - 10.3389/fbioe.2026.1926795
UR - https://doi.org/10.3389/fbioe.2026.1926795
AB - IntroductionConventional industrial biotechnology remains constrained by contamination control requirements and energy-intensive biomass separation. Next-generation industrial biotechnology (NGIB) seeks microbial platforms that retain useful production phenotypes under open cultivation while enabling simpler process configurations. Here, we evaluated Pseudomonas sp. ABC1, a fluorescent siderophore-producing bacterium, as a discovery-stage ecological chassis for open bioproduction.MethodsSeven-day open, non-sterile static cultivation was integrated with community profiling, microscopy, elemental analysis, peptidomics, proteomics, and comparative genomics.ResultsABC1 formed a cohesive floating pellicle associated with a non-axenic bacterial consortium that included Pseudomonas, Delftia, Flavobacterium, Acidovorax, and additional environmental genera. Genome-informed reconstruction of representative community members indicated distributed capacities for iron acquisition, oxidative-stress protection, adhesion, nutrient acquisition, and extracellular-matrix metabolism. LC-MS/MS recovered 1,518 de novo peptide sequences, including 1,478 non-redundant sequences, with recurrent N-hydroxy-cyclo-ornithine derivatives and other modified peptide forms. These signatures are consistent with metallophore-associated biosynthesis predicted from the extensive iron-acquisition machinery previously described for ABC1. Comparative genomics further showed that ABC1 lacks complete canonical Pel, Psl, alginate, and PNAG systems but contains two extended loci encoding complementary functions for non-canonical extracellular-polysaccharide assembly, including rfbDCAB precursor synthesis, multiple glycosyltransferases, candidate Wzx/Wzy-like proteins, and export-associated components. ABC1 also encodes an expanded nutrient-acquisition architecture and 29 cyclic-di-GMP-associated proteins. Calcium-enriched pellicle microdomains and extracellular adhesive proteins provide additional candidate structural features, although mineral identity and polymer composition remain unresolved.DiscussionCollectively, these data support ABC1 as a promising discovery-stage system in which ecological selection, extracellular-matrix organization, and biosynthetic diversification can be investigated under open cultivation. Quantitative validation of productivity, contamination resistance, biomass-recovery performance, safety, and scale-up remains necessary before industrial advantage can be established.
SN - 2296-4185
ER -