Genome-Wide Identification and Expression Profiling of PYL Genes in Brassica napus Under ABA and Drought-Stress Treatments
4 affiliations
- 1Institute of Vegetable Science, Zhejiang University, Hangzhou 310058, China
- 2West Tennessee AgResearch & Education Center (WTREC), Department of Plant Science, University of Tennessee, Knoxville, TN 38301, USA
- 3College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China
- 4School of Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China
Abstract
Brassica napus L. is a major oilseed crop whose productivity is significantly affected by abiotic stresses such as drought. PYR/PYL/RCAR (PYL) proteins act as key abscisic acid (ABA) receptors and play central roles in stress responses. However, a comprehensive genome-wide analysis of the PYL gene family in B. napus is still lacking, limiting our understanding of their functions in plant and stress adaptation. This study reports the first comprehensive genome-wide analysis of the PYL gene family in B. napus (rapeseed), cultivar ZS11, identifying 25 BnPYL genes grouped into four subfamilies, I (four genes), I-II (five genes), II (five genes), III (11 genes), and their encoded proteins were predicted to be mainly localized in the chloroplast. Structural analysis revealed diverse exon–intron organization and 10 conserved motifs. All identified BnPYLs contained Polyketide_cyc2 domains (PF10604), supporting their annotation as members of the PYL family. Promoter analysis identified cis-regulatory elements related to light response, stress regulation, and hormonal signaling. Computational analysis of post-translational modifications suggested that phosphorylation sites are mainly localized at serine and threonine residues. Tertiary structure modelling revealed conserved three-dimensional architectures among BnPYL proteins, suggesting potential functional conservation. Expression profiling and RT-qPCR analyses revealed that several BnPYL genes respond to ABA-mediated drought stress, with BnPYL15 and BnPYL22 exhibiting the highest induction (4–5-fold) and BnPYL2, BnPYL5, BnPYL6, BnPYL17, BnPYL18, and BnPYL25 showing significant upregulation (2.0–4.5-fold), suggesting potential roles in enhancing drought tolerance in B. napus.
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BibTeX
@article{gulzar2026genomewide,
title = {{Genome-Wide Identification and Expression Profiling of PYL Genes in Brassica napus Under ABA and Drought-Stress Treatments}},
author = {Rana Muhammad Amir Gulzar and Nazir Ahmad and Xiaohong Zhao and Tong Mao Zhao and Jianyin Zhan and Hongrui Yu and Muhammad Haseeb Javaid and Raheel Munir and Muhammad Mudassir Nazir and Iqbal Hussain},
journal = {Stresses},
year = {2026},
month = jun,
doi = {10.3390/stresses6030041},
url = {https://doi.org/10.3390/stresses6030041},
note = {CC BY},
}
RIS
TY - JOUR
TI - Genome-Wide Identification and Expression Profiling of PYL Genes in Brassica napus Under ABA and Drought-Stress Treatments
AU - Rana Muhammad Amir Gulzar
AU - Nazir Ahmad
AU - Xiaohong Zhao
AU - Tong Mao Zhao
AU - Jianyin Zhan
AU - Hongrui Yu
AU - Muhammad Haseeb Javaid
AU - Raheel Munir
AU - Muhammad Mudassir Nazir
AU - Iqbal Hussain
PY - 2026
DA - 2026/06/27
JO - Stresses
DO - 10.3390/stresses6030041
UR - https://doi.org/10.3390/stresses6030041
AB - Brassica napus L. is a major oilseed crop whose productivity is significantly affected by abiotic stresses such as drought. PYR/PYL/RCAR (PYL) proteins act as key abscisic acid (ABA) receptors and play central roles in stress responses. However, a comprehensive genome-wide analysis of the PYL gene family in B. napus is still lacking, limiting our understanding of their functions in plant and stress adaptation. This study reports the first comprehensive genome-wide analysis of the PYL gene family in B. napus (rapeseed), cultivar ZS11, identifying 25 BnPYL genes grouped into four subfamilies, I (four genes), I-II (five genes), II (five genes), III (11 genes), and their encoded proteins were predicted to be mainly localized in the chloroplast. Structural analysis revealed diverse exon–intron organization and 10 conserved motifs. All identified BnPYLs contained Polyketide_cyc2 domains (PF10604), supporting their annotation as members of the PYL family. Promoter analysis identified cis-regulatory elements related to light response, stress regulation, and hormonal signaling. Computational analysis of post-translational modifications suggested that phosphorylation sites are mainly localized at serine and threonine residues. Tertiary structure modelling revealed conserved three-dimensional architectures among BnPYL proteins, suggesting potential functional conservation. Expression profiling and RT-qPCR analyses revealed that several BnPYL genes respond to ABA-mediated drought stress, with BnPYL15 and BnPYL22 exhibiting the highest induction (4–5-fold) and BnPYL2, BnPYL5, BnPYL6, BnPYL17, BnPYL18, and BnPYL25 showing significant upregulation (2.0–4.5-fold), suggesting potential roles in enhancing drought tolerance in B. napus.
SN - 2673-7140
ER -