Transcriptomic analysis of genotypes derived from Rosa wichurana unveils molecular mechanisms associated with quantitative resistance to Diplocarpon rosae
Abstract
Black spot disease, caused by the hemibiotrophic fungus Diplocarpon rosae, is a major constraint for garden rose cultivation. Developing resistant cultivars requires a better understanding of the genetic and molecular mechanisms underlying resistance. A hybrid of Rosa wichurana (RW) displays quantitative resistance to black spot that has been previously associated with two major Quantitative Trait Loci (QTLs), B3 and B5, located on linkage groups 3 and 5, respectively. Our objective was to investigate the molecular mechanisms associated with these QTLs by comparing the transcriptome of RW and four F1 individuals carrying different QTL combinations. Differential expression analyses comparing inoculated and mock-treated samples within each genotype revealed that the strongest transcriptional response occurred at 3 days post-inoculation, which was therefore considered the most informative stage for functional interpretation. Genotypes carrying QTL B3 displayed transcriptional signatures consistent with pathogen perception, defense signaling, reactive oxygen species production, cell wallassociated responses and localized cell death. In contrast, genotypes carrying QTL B5 shared relatively fewer transcriptional responses, preventing the identification of a clear QTL-specific contribution. Overall, these results provide new insight into the molecular basis of quantitative resistance to black spot in rose and suggest that resistance inherited from RW involves coordinated and background-dependent regulation of multiple defenserelated processes.
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BibTeX
@article{lambelin2026transcriptomic,
title = {{Transcriptomic analysis of genotypes derived from Rosa wichurana unveils molecular mechanisms associated with quantitative resistance to Diplocarpon rosae}},
author = {Laurine Lambelin and Tatiana Thouroude and Julien Jeauffre and Jérôme Chameau and Christophe Vilfroy and Christine Boursier and Sébastien Aubourg and Sandra Pelletier and Diana Carolina Lopez Arias and Laurence Hibrand-Saint Oyant and Vanessa Soufflet-Freslon and Fabrice Foucher and Sophie Paillard},
journal = {Physiological and Molecular Plant Pathology},
year = {2026},
month = sep,
doi = {10.1016/j.pmpp.2026.103464},
url = {https://univ-angers.hal.science/hal-05746030v1},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Transcriptomic analysis of genotypes derived from Rosa wichurana unveils molecular mechanisms associated with quantitative resistance to Diplocarpon rosae
AU - Laurine Lambelin
AU - Tatiana Thouroude
AU - Julien Jeauffre
AU - Jérôme Chameau
AU - Christophe Vilfroy
AU - Christine Boursier
AU - Sébastien Aubourg
AU - Sandra Pelletier
AU - Diana Carolina Lopez Arias
AU - Laurence Hibrand-Saint Oyant
AU - Vanessa Soufflet-Freslon
AU - Fabrice Foucher
AU - Sophie Paillard
PY - 2026
DA - 2026/09/10
JO - Physiological and Molecular Plant Pathology
DO - 10.1016/j.pmpp.2026.103464
UR - https://univ-angers.hal.science/hal-05746030v1
AB - Black spot disease, caused by the hemibiotrophic fungus Diplocarpon rosae, is a major constraint for garden rose cultivation. Developing resistant cultivars requires a better understanding of the genetic and molecular mechanisms underlying resistance. A hybrid of Rosa wichurana (RW) displays quantitative resistance to black spot that has been previously associated with two major Quantitative Trait Loci (QTLs), B3 and B5, located on linkage groups 3 and 5, respectively. Our objective was to investigate the molecular mechanisms associated with these QTLs by comparing the transcriptome of RW and four F1 individuals carrying different QTL combinations. Differential expression analyses comparing inoculated and mock-treated samples within each genotype revealed that the strongest transcriptional response occurred at 3 days post-inoculation, which was therefore considered the most informative stage for functional interpretation. Genotypes carrying QTL B3 displayed transcriptional signatures consistent with pathogen perception, defense signaling, reactive oxygen species production, cell wallassociated responses and localized cell death. In contrast, genotypes carrying QTL B5 shared relatively fewer transcriptional responses, preventing the identification of a clear QTL-specific contribution. Overall, these results provide new insight into the molecular basis of quantitative resistance to black spot in rose and suggest that resistance inherited from RW involves coordinated and background-dependent regulation of multiple defenserelated processes.
SN - 0885-5765
SN - 1096-1178
ER -