Prospecting for efflux pump and repressor genes in rivers of the Amazon region of Brazil.
8 affiliations
- 1Institute of Socioenvironmental and Water Resources, Federal Rural University of the Amazon, Belém, Brazil.
- 2Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, 31275-013, Brazil.
- 3Laboratory of Simulation and Computational Biology, Pará Center for High Performance Distributed Computing (CCAD), Federal University of Pará UFPA, Belém, Brazil.
- 4Laboratory of Genetics and Conservation, Institute of Coastal Studies, Federal University of Pará (UFPA), Bragança, Brazil.
- 5Institute of Biological Sciences, Federal University of Pará, Belém, Brazil.
- 6Institute of Biology (Microbiology) & Institute of Health Sciences, Federal Universityof Bahia (UFBA), Salvador, Brazil.
- 7Institute of Biological Sciences, Federal University of Pará, Belém, Brazil. [email protected].
- 8Laboratory of Simulation and Computational Biology, Pará Center for High Performance Distributed Computing (CCAD), Federal University of Pará UFPA, Belém, Brazil. [email protected].
Abstract
Background
The spread of multidrug-resistant bacteria in water bodies poses a significant public health concern. Efflux pumps represent critical mechanisms of antimicrobial resistance, regulated by genetic repressors that aid bacterial adaptation to environmental stress. This study profiles the diversity of efflux pump genes and genetic repressors in the surface waters of the Cereja (Bragança) and Uraim (Paragominas) rivers in the Amazon region of Brazil.Methods and results
Surface water samples were collected along a gradient of anthropogenic impact, and environmental DNA was sequenced on the Illumina platform. The resulting sequencing data were subsequently subjected to metagenomic analysis, which identified a higher abundance of efflux pump genes in the Cereja River compared to the Uraim River, dominated by the RND and SMR families. Additionally, two-component system (TCS) repressor genes, particularly cbrB, were successfully recovered and modeled using Swiss-Model homology modeling.Conclusions
The higher coverage of the cbrB repressor gene in anthropogenically impacted sites underscores its regulatory role in bacterial stress responses, suggesting its potential application as a novel biomarker of environmental pollution and antibiotic resistance spread in aquatic ecosystems.
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BibTeX
@article{chaves2026prospecting,
title = {{Prospecting for efflux pump and repressor genes in rivers of the Amazon region of Brazil.}},
author = {Valéria Alves Chaves and David Tavares Martins and Carlos Willian Dias Dantas and Savia Moreira da Silva and Juliana Araripe Gomes da Silva and Ana Lídia Queiroz Cavalcante and Daralyns Borges Macedo and Ana Luzia Leite Fernandes and Sandeep Tiwari and Rommel Thiago Jucá Ramos and Oscar Victor Cardenas-Alegria},
journal = {Molecular biology reports},
year = {2026},
month = oct,
doi = {10.1007/s11033-026-12979-1},
url = {https://europepmc.org/article/MED/42909663},
note = {CC BY},
}
RIS
TY - JOUR
TI - Prospecting for efflux pump and repressor genes in rivers of the Amazon region of Brazil.
AU - Valéria Alves Chaves
AU - David Tavares Martins
AU - Carlos Willian Dias Dantas
AU - Savia Moreira da Silva
AU - Juliana Araripe Gomes da Silva
AU - Ana Lídia Queiroz Cavalcante
AU - Daralyns Borges Macedo
AU - Ana Luzia Leite Fernandes
AU - Sandeep Tiwari
AU - Rommel Thiago Jucá Ramos
AU - Oscar Victor Cardenas-Alegria
PY - 2026
DA - 2026/10/23
JO - Molecular biology reports
DO - 10.1007/s11033-026-12979-1
UR - https://europepmc.org/article/MED/42909663
AB - Background The spread of multidrug-resistant bacteria in water bodies poses a significant public health concern. Efflux pumps represent critical mechanisms of antimicrobial resistance, regulated by genetic repressors that aid bacterial adaptation to environmental stress. This study profiles the diversity of efflux pump genes and genetic repressors in the surface waters of the Cereja (Bragança) and Uraim (Paragominas) rivers in the Amazon region of Brazil.Methods and results Surface water samples were collected along a gradient of anthropogenic impact, and environmental DNA was sequenced on the Illumina platform. The resulting sequencing data were subsequently subjected to metagenomic analysis, which identified a higher abundance of efflux pump genes in the Cereja River compared to the Uraim River, dominated by the RND and SMR families. Additionally, two-component system (TCS) repressor genes, particularly cbrB, were successfully recovered and modeled using Swiss-Model homology modeling.Conclusions The higher coverage of the cbrB repressor gene in anthropogenically impacted sites underscores its regulatory role in bacterial stress responses, suggesting its potential application as a novel biomarker of environmental pollution and antibiotic resistance spread in aquatic ecosystems.
SN - 0301-4851
SN - 1573-4978
ER -