Immune modulation by lipopolysaccharide preconditioning: A novel approach to neuropathic pain in rats.
- 1Neuroscience Research Center, Biomedical Research Institute, Golestan University of Medical Science, Gorgan, Iran.
Abstract
Neuropathic pain (NPP) resulting from peripheral nerve compression is associated with heightened nociceptive sensitivity and increased neuroinflammation. This study investigated the effects of lipopolysaccharide (LPS) preconditioning on behavioral and inflammatory outcomes in a rat model of the chronic constriction injury (CCI)-induced NPP. Animals were assigned to groups including Control, CCI (underwent CCI surgery), LPS (0.01 mg/kg) + CCI, LPS (0.1 mg/kg) + CCI, and LPS (0.5 mg/kg) + CCI, which received LPS intraperitoneally and then underwent CCI surgery. Pain-related behaviors were assessed using the Acetone Test and Hot Plate Test on days 7 and 14 after CCI induction. Then, Spinal cord samples were collected to quantify tumor necrosis factor alpha (TNFα) levels, a key pro-inflammatory mediator. CCI induction significantly increased hypersensitivity to pain, accompanied by elevated TNF-α expression in the spinal cord. LPS preconditioning attenuated pain behaviors and reduced TNF-α levels compared with the CCI group. These findings suggest that LPS preconditioning modulates neuroimmune responses and may confer protective effects against NPP development induced by peripheral nerve compression.
Related
- Lipopolysaccharide-induced inflammatory modulation of the apoptotic pathways in human primary aniridia limbal stromal cells, in vitro
- Maternal Immune Activation by Lipopolysaccharide Reduced Parvalbumin Levels and Increased Perineuronal Nets in the Prefrontal Cortex Without Inducing Schizophrenia-like Behavioral Deficits in PV-Cre Long-Evans Rats
- mRNA-based nanomedicine for diabetic wound repair: advances in LNP delivery, immune modulation, and regenerative pathways
- Effects of RhBNP combined with immune modulation on immune function and short-term clinical outcomes in patients with atrial fibrillation and heart failure
- New strategies for the treatment of tuberculosis: research progress and clinical translation in immune modulation and inflammation control
BibTeX
@article{hesam2026immune,
title = {{Immune modulation by lipopolysaccharide preconditioning: A novel approach to neuropathic pain in rats.}},
author = {Soqra Hesam and Fatemeh Piri and Mahshid Moradpour and Hamid Sepehri},
journal = {IBRO neuroscience reports},
year = {2026},
month = oct,
doi = {10.1016/j.ibneur.2026.10.006},
url = {https://europepmc.org/article/MED/42999381},
note = {CC BY-NC-ND},
}
RIS
TY - JOUR
TI - Immune modulation by lipopolysaccharide preconditioning: A novel approach to neuropathic pain in rats.
AU - Soqra Hesam
AU - Fatemeh Piri
AU - Mahshid Moradpour
AU - Hamid Sepehri
PY - 2026
DA - 2026/10/24
JO - IBRO neuroscience reports
DO - 10.1016/j.ibneur.2026.10.006
UR - https://europepmc.org/article/MED/42999381
AB - Neuropathic pain (NPP) resulting from peripheral nerve compression is associated with heightened nociceptive sensitivity and increased neuroinflammation. This study investigated the effects of lipopolysaccharide (LPS) preconditioning on behavioral and inflammatory outcomes in a rat model of the chronic constriction injury (CCI)-induced NPP. Animals were assigned to groups including Control, CCI (underwent CCI surgery), LPS (0.01 mg/kg) + CCI, LPS (0.1 mg/kg) + CCI, and LPS (0.5 mg/kg) + CCI, which received LPS intraperitoneally and then underwent CCI surgery. Pain-related behaviors were assessed using the Acetone Test and Hot Plate Test on days 7 and 14 after CCI induction. Then, Spinal cord samples were collected to quantify tumor necrosis factor alpha (TNFα) levels, a key pro-inflammatory mediator. CCI induction significantly increased hypersensitivity to pain, accompanied by elevated TNF-α expression in the spinal cord. LPS preconditioning attenuated pain behaviors and reduced TNF-α levels compared with the CCI group. These findings suggest that LPS preconditioning modulates neuroimmune responses and may confer protective effects against NPP development induced by peripheral nerve compression.
SN - 2667-2421
ER -