Repressed mTORC1 signaling and transient dendritic pruning support axonal regeneration in adult zebrafish
Abstract
Dendrite degeneration is an early pathological feature following axonal damage, yet its role during successful axonal regeneration remains poorly understood. Using sparse labeling of retinal ganglion cells in adult zebrafish, we show that axonal regeneration is accompanied by a transient phase of dendritic pruning following optic nerve crush. Dendritic pruning occurs during axon elongation and is reversed after brain reinnervation. Although insulin-mechanistic target of rapamycin (mTOR) signaling is rapidly upregulated immediately after injury, it is subsequently suppressed, coinciding with dendritic pruning and sustained axonal growth. Prolonging mTOR activation through insulin treatment or direct pathway stimulation reduces dendritic pruning in an mTORC1-dependent manner but significantly delays axonal regrowth. Together, these findings identify dendritic remodeling as a temporally regulated component of axonal regeneration in zebrafish and reveal a functional trade-off between dendritic preservation and axon regrowth. This warrants further investigation on whether temporal modulation of dendritic remodeling could promote axonal regeneration in the mammalian central nervous system (CNS).
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BibTeX
@article{zhang2026repressed,
title = {{Repressed mTORC1 signaling and transient dendritic pruning support axonal regeneration in adult zebrafish}},
author = {Anyi Zhang and Luca Masin and Steven Bergmans and Elena Putti and Shahad Albadri and Fabienne E. Poulain and Filippo Del Bene and Lieve Moons},
journal = {iScience},
year = {2026},
month = oct,
doi = {10.1016/j.isci.2026.117826},
url = {https://www.cell.com/iscience/fulltext/S2589-0042(26)03207-4},
note = {CC BY-NC-ND 4.0},
}
RIS
TY - JOUR
TI - Repressed mTORC1 signaling and transient dendritic pruning support axonal regeneration in adult zebrafish
AU - Anyi Zhang
AU - Luca Masin
AU - Steven Bergmans
AU - Elena Putti
AU - Shahad Albadri
AU - Fabienne E. Poulain
AU - Filippo Del Bene
AU - Lieve Moons
PY - 2026
DA - 2026/10/08
JO - iScience
DO - 10.1016/j.isci.2026.117826
UR - https://www.cell.com/iscience/fulltext/S2589-0042(26)03207-4
AB - Dendrite degeneration is an early pathological feature following axonal damage, yet its role during successful axonal regeneration remains poorly understood. Using sparse labeling of retinal ganglion cells in adult zebrafish, we show that axonal regeneration is accompanied by a transient phase of dendritic pruning following optic nerve crush. Dendritic pruning occurs during axon elongation and is reversed after brain reinnervation. Although insulin-mechanistic target of rapamycin (mTOR) signaling is rapidly upregulated immediately after injury, it is subsequently suppressed, coinciding with dendritic pruning and sustained axonal growth. Prolonging mTOR activation through insulin treatment or direct pathway stimulation reduces dendritic pruning in an mTORC1-dependent manner but significantly delays axonal regrowth. Together, these findings identify dendritic remodeling as a temporally regulated component of axonal regeneration in zebrafish and reveal a functional trade-off between dendritic preservation and axon regrowth. This warrants further investigation on whether temporal modulation of dendritic remodeling could promote axonal regeneration in the mammalian central nervous system (CNS).
SN - 2589-0042
ER -