Recent advances in protein extraction methods from bone tissue for proteomics analysis
- 1Universidade Estadual Paulista Julio de Mesquita Filho 1 , Araraquara, São Paulo, Brazil
- 2Pontifica Universidade Católica de Campinas 2 , Campinas, São Paulo, Brazil
- 3Universidad San Francisco de Quito 3 , Quito, Pichincha, Ecuador
Abstract
Proteomic analysis is a fundamental tool for obtaining new information about biological functions that occur at the molecular level in the body, allowing the understanding of physiological and pathological processes, as well as contributing to the diagnosis and prevention of diseases. However, a critical step for the success of these analyses is the protein extraction from the tissue of interest, with well-described and reproducible protocols still scarce in the current literature, especially for bone tissues. Therefore, the main objective of this study was to present a reliable and standardized methodology for protein extraction from hard tissue samples. The methodology employed was based on an extraction protocol using demineralized bone tissue, with more detailed experimental parameters such as incubation times, temperatures and sample storage conditions. As a result, 1429 proteins previously described for the species Rattus norvegicus were identified, along with 138 uncharacterized proteins and the detection of signalling pathways strongly associated with bone metabolism. The proposed protocol has the potential for adaptation to different experimental contexts, being adjustable for various species and types of bone, considering variations in density, degree of mineralization and structural characteristics of the analysed tissue.
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BibTeX
@article{nascimento2026recent,
title = {{Recent advances in protein extraction methods from bone tissue for proteomics analysis}},
author = {Karoline B. do. Nascimento and Bruno L. Graciliano Silva and Felipe E. Pinotti and Julio Cesar Sánchez-Puetate and Morgana R. Guimarães-Stabili and Camila C. Marcantonio and Rosemary Adriana C. Marcantonio},
journal = {Royal Society Open Science},
year = {2026},
month = oct,
doi = {10.1098/rsos.261692},
url = {https://royalsocietypublishing.org/rsos/article/13/10/261692/483585/Recent-advances-in-protein-extraction-methods-from},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Recent advances in protein extraction methods from bone tissue for proteomics analysis
AU - Karoline B. do. Nascimento
AU - Bruno L. Graciliano Silva
AU - Felipe E. Pinotti
AU - Julio Cesar Sánchez-Puetate
AU - Morgana R. Guimarães-Stabili
AU - Camila C. Marcantonio
AU - Rosemary Adriana C. Marcantonio
PY - 2026
DA - 2026/10/14
JO - Royal Society Open Science
DO - 10.1098/rsos.261692
UR - https://royalsocietypublishing.org/rsos/article/13/10/261692/483585/Recent-advances-in-protein-extraction-methods-from
AB - Proteomic analysis is a fundamental tool for obtaining new information about biological functions that occur at the molecular level in the body, allowing the understanding of physiological and pathological processes, as well as contributing to the diagnosis and prevention of diseases. However, a critical step for the success of these analyses is the protein extraction from the tissue of interest, with well-described and reproducible protocols still scarce in the current literature, especially for bone tissues. Therefore, the main objective of this study was to present a reliable and standardized methodology for protein extraction from hard tissue samples. The methodology employed was based on an extraction protocol using demineralized bone tissue, with more detailed experimental parameters such as incubation times, temperatures and sample storage conditions. As a result, 1429 proteins previously described for the species Rattus norvegicus were identified, along with 138 uncharacterized proteins and the detection of signalling pathways strongly associated with bone metabolism. The proposed protocol has the potential for adaptation to different experimental contexts, being adjustable for various species and types of bone, considering variations in density, degree of mineralization and structural characteristics of the analysed tissue.
SN - 2054-5703
ER -