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Peer-reviewedCC BYCell proliferation

No Female Germline Stem Cells Were Identified by Advanced Dual Recombinase-Based Lineage Tracing System.

  • Yang Gao1,2
  • Zhiming Shen3,4
  • Xiuhong Cui1,2,5
  • Min Chen1,2,5
  • Mengyue Wang6
  • Kui Liu7,8
  • Fei Gao1,2,5
  • Hongmei Wang1,2,5
8 affiliations
  1. 1State Key Laboratory of Organ Regeneration and Reconstruction, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
  2. 2University of Chinese Academy of Sciences, Beijing, China.
  3. 3Research Institute for Marine Traditional Chinese Medicine (Qingdao Academy of Chinese Medical Sciences), the SATCM'S Key Unit of Discovering and Developing New Marine TCM Drugs, Key Laboratory of Marine Traditional Chinese Medicine in Shandong Universities, Shandong University of Traditional Chinese Medicine, Jinan, China.
  4. 4Shandong University of Traditional Chinese Medicine Qingdao Academy of Chinese Medical Sciences, Qingdao Key Laboratory of Research in Marine Traditional Chinese Medicine, Qingdao Key Technology Innovation Center of Marine Traditional Chinese Medicine's Deep Development and Industrialization, Qingdao, China.
  5. 5Beijing Institute for Stem Cell and Regenerative Medicine, Beijing, China.
  6. 6State Key Laboratory of Reproductive Medicine and Offspring Health, Center for Reproductive Medicine, Institute of Women, Children and Reproductive Health, Shandong University, Jinan, China.
  7. 7Department of Obstetrics and Gynecology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China.
  8. 8Shenzhen Key Laboratory of Fertility Regulation, Center of Assisted Reproduction and Embryology, The University of Hong Kong-Shenzhen Hospital, Shenzhen, Guangdong, China.
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Abstract

Whether female germline stem cells (fGSCs) exist in postnatal mammalian ovaries has been debated for decades. Since the fGSCs were first reported in 2004, a large number of studies have been conducted using different approaches. However, the persistent controversy surrounding the results has caused confusion among many researchers and clinicians. To clearly clarify this question, we developed advanced tracing system to label any possible fGSCs in adult female mice. Our results showed that no Stra8-expressing germ cells were detected in the ovaries of adult female mice using a long-term uninterrupted lineage tracing system under both physiological and pathological conditions. Furthermore, we adopted the interleaved reporter tracing system that allows mutually exclusive labelling of germ cells and non-germ cell populations while preventing potential secondary recombination. This approach revealed no evidence of de novo germ cell formation during postnatal life. Together, our findings provide the evidence that fGSCs and postnatal oogenesis do not exist under both physiological and pathological conditions in mouse models, therefore reinforcing the notion that the primordial follicle pool established perinatally serves as the primary source of oocytes through the entire reproductive lifespan.

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Cite

BibTeXRIS
BibTeX
@article{gao2026female,
  title = {{No Female Germline Stem Cells Were Identified by Advanced Dual Recombinase-Based Lineage Tracing System.}},
  author = {Yang Gao and Zhiming Shen and Xiuhong Cui and Min Chen and Mengyue Wang and Kui Liu and Fei Gao and Hongmei Wang},
  journal = {Cell proliferation},
  year = {2026},
  month = oct,
  doi = {10.1111/cpr.70289},
  url = {https://europepmc.org/article/MED/42901482},
  note = {CC BY},
}
RIS
TY  - JOUR
TI  - No Female Germline Stem Cells Were Identified by Advanced Dual Recombinase-Based Lineage Tracing System.
AU  - Yang Gao
AU  - Zhiming Shen
AU  - Xiuhong Cui
AU  - Min Chen
AU  - Mengyue Wang
AU  - Kui Liu
AU  - Fei Gao
AU  - Hongmei Wang
PY  - 2026
DA  - 2026/10/20
JO  - Cell proliferation
DO  - 10.1111/cpr.70289
UR  - https://europepmc.org/article/MED/42901482
AB  - Whether female germline stem cells (fGSCs) exist in postnatal mammalian ovaries has been debated for decades. Since the fGSCs were first reported in 2004, a large number of studies have been conducted using different approaches. However, the persistent controversy surrounding the results has caused confusion among many researchers and clinicians. To clearly clarify this question, we developed advanced tracing system to label any possible fGSCs in adult female mice. Our results showed that no Stra8-expressing germ cells were detected in the ovaries of adult female mice using a long-term uninterrupted lineage tracing system under both physiological and pathological conditions. Furthermore, we adopted the interleaved reporter tracing system that allows mutually exclusive labelling of germ cells and non-germ cell populations while preventing potential secondary recombination. This approach revealed no evidence of de novo germ cell formation during postnatal life. Together, our findings provide the evidence that fGSCs and postnatal oogenesis do not exist under both physiological and pathological conditions in mouse models, therefore reinforcing the notion that the primordial follicle pool established perinatally serves as the primary source of oocytes through the entire reproductive lifespan.
SN  - 0960-7722
SN  - 1365-2184
ER  -