Non-Invasive Transcriptomic Profiling of Inflammatory Skin Diseases Using Four Tape Strips.
4 affiliations
- 1Department of Dermatology and Allergy, Copenhagen University Hospital, Herlev and Gentofte, 2900 Hellerup, Denmark.
- 2Department of Clinical Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen N, Denmark.
- 3The Leo Foundation Skin Immunology Research Center, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen N, Denmark.
- 4The National Allergy Research Centre, Department of Dermatology and Allergy, Copenhagen University Hospital, Herlev and Gentofte, 2900 Hellerup, Denmark.
Abstract
Tape strip-based transcriptomics shows promise for precision medicine in inflammatory skin diseases, but methodological variation limits reproducibility and clinical use. This study investigates whether four or eight tape strips are sufficient for transcriptomic profiling across three inflammatory skin diseases. As part of the Danish cohort study BIOSKIN, patients with atopic dermatitis (AD), contact dermatitis (CD), psoriasis, and healthy controls (n = 8 per group) were enrolled. Two sets of eight consecutive tape strips were obtained from each participant from lesional skin (patients) or healthy skin (controls) and pooled for RNA sequencing as strips 1-8, 1-4, or 5-8. Despite low RNA yield, sequencing was successful across all disease groups. Gene detectability was consistent across tape strip strategies, with 78.1%, 73.1%, and 67.7% overlap across strips 1-4, 5-8, and 1-8 for psoriasis, AD, and CD, respectively. Principal component analysis distinguished patients with psoriasis and AD from healthy controls, and enrichment analysis captured biologically relevant pathways across all three disease groups. In psoriasis, tape strip transcriptomics captured a core psoriatic transcriptome consistent with paired biopsy data. Four tape strips were sufficient for RNA sequencing and detection of disease-relevant inflammatory responses, supporting their use in longitudinal cohort studies and split-sampling designs. Performance was most robust for psoriasis and AD; optimization may benefit CD given the challenges of palmar sampling.
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BibTeX
@article{vinge2026noninvasive,
title = {{Non-Invasive Transcriptomic Profiling of Inflammatory Skin Diseases Using Four Tape Strips.}},
author = {Anna Schultz Vinge and Sule Altintas and Niels Ødum and Jeanne Duus Johansen and Lone Skov and Marianne Bengtson Løvendorf},
journal = {International journal of molecular sciences},
year = {2026},
month = sep,
doi = {10.3390/ijms27198544},
url = {https://europepmc.org/article/MED/42883791},
note = {CC BY},
}
RIS
TY - JOUR
TI - Non-Invasive Transcriptomic Profiling of Inflammatory Skin Diseases Using Four Tape Strips.
AU - Anna Schultz Vinge
AU - Sule Altintas
AU - Niels Ødum
AU - Jeanne Duus Johansen
AU - Lone Skov
AU - Marianne Bengtson Løvendorf
PY - 2026
DA - 2026/09/24
JO - International journal of molecular sciences
DO - 10.3390/ijms27198544
UR - https://europepmc.org/article/MED/42883791
AB - Tape strip-based transcriptomics shows promise for precision medicine in inflammatory skin diseases, but methodological variation limits reproducibility and clinical use. This study investigates whether four or eight tape strips are sufficient for transcriptomic profiling across three inflammatory skin diseases. As part of the Danish cohort study BIOSKIN, patients with atopic dermatitis (AD), contact dermatitis (CD), psoriasis, and healthy controls (n = 8 per group) were enrolled. Two sets of eight consecutive tape strips were obtained from each participant from lesional skin (patients) or healthy skin (controls) and pooled for RNA sequencing as strips 1-8, 1-4, or 5-8. Despite low RNA yield, sequencing was successful across all disease groups. Gene detectability was consistent across tape strip strategies, with 78.1%, 73.1%, and 67.7% overlap across strips 1-4, 5-8, and 1-8 for psoriasis, AD, and CD, respectively. Principal component analysis distinguished patients with psoriasis and AD from healthy controls, and enrichment analysis captured biologically relevant pathways across all three disease groups. In psoriasis, tape strip transcriptomics captured a core psoriatic transcriptome consistent with paired biopsy data. Four tape strips were sufficient for RNA sequencing and detection of disease-relevant inflammatory responses, supporting their use in longitudinal cohort studies and split-sampling designs. Performance was most robust for psoriasis and AD; optimization may benefit CD given the challenges of palmar sampling.
SN - 1422-0067
ER -