Myosin-9b Controls Epithelial Brush Border Architecture through Motility-Dependent RhoA Signaling
- 1University at Buffalo
Abstract
Genetic variations in the MYO9B gene have been associated with Crohn's disease, celiac disease, and ulcerative colitis. These diseases have been characterized as primarily immune disorders. However, the overall molecular basis for the influence of Myo9b in these diseases remains poorly understood. Using in vivo small intestine ileum and human cell culture models, we identify a molecular function for Myo9b in the regulation of epithelial brush border microvilli. Using live-cell super-resolution microscopy, we characterize the motility of Myo9b as it moves toward enriched puncta at the tips of microvilli and visualize its direct regulation of small GTPase signaling using an active RhoA biosensor. In Myo9b knockout cells, microvilli abundance and dynamics are altered, but the cells ultimately maintain the presence of microvilli and the appropriate incorporation of microvilli specific cytoskeletal to membrane regulators such as Ezrin. Alternatively, expression of the Myo9b-S1011A disease variant as the only genetic copy in human cells results in a total loss of microvilli and Ezrin apical localization. These results indicate that Myo9b is a critical regulator of epithelial cell morphology and microvilli. Further, our data establish that the S1011A disease variant disrupts microvilli in human cells, suggesting a potential mechanistic link to its involvement in disease states.
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BibTeX
@misc{murray2026myosin9b,
title = {{Myosin-9b Controls Epithelial Brush Border Architecture through Motility-Dependent RhoA Signaling}},
author = {E. C. Murray and N. Manoj and L. Ziegler and G. M. Hodge and S. Fraher and L. S. Lee and L. Code and S. A. Lewis and C. E. Schaner Tooley and Y. Bae and J. Konen and J. E. Bard and A. T. Lombardo},
howpublished = {bioRxiv},
year = {2026},
month = sep,
doi = {10.64898/2026.09.08.750145},
url = {https://www.biorxiv.org/content/10.64898/2026.09.08.750145v1},
note = {CC BY},
}
RIS
TY - UNPB
TI - Myosin-9b Controls Epithelial Brush Border Architecture through Motility-Dependent RhoA Signaling
AU - E. C. Murray
AU - N. Manoj
AU - L. Ziegler
AU - G. M. Hodge
AU - S. Fraher
AU - L. S. Lee
AU - L. Code
AU - S. A. Lewis
AU - C. E. Schaner Tooley
AU - Y. Bae
AU - J. Konen
AU - J. E. Bard
AU - A. T. Lombardo
PY - 2026
DA - 2026/09/08
PB - bioRxiv
DO - 10.64898/2026.09.08.750145
UR - https://www.biorxiv.org/content/10.64898/2026.09.08.750145v1
AB - Genetic variations in the MYO9B gene have been associated with Crohn's disease, celiac disease, and ulcerative colitis. These diseases have been characterized as primarily immune disorders. However, the overall molecular basis for the influence of Myo9b in these diseases remains poorly understood. Using in vivo small intestine ileum and human cell culture models, we identify a molecular function for Myo9b in the regulation of epithelial brush border microvilli. Using live-cell super-resolution microscopy, we characterize the motility of Myo9b as it moves toward enriched puncta at the tips of microvilli and visualize its direct regulation of small GTPase signaling using an active RhoA biosensor. In Myo9b knockout cells, microvilli abundance and dynamics are altered, but the cells ultimately maintain the presence of microvilli and the appropriate incorporation of microvilli specific cytoskeletal to membrane regulators such as Ezrin. Alternatively, expression of the Myo9b-S1011A disease variant as the only genetic copy in human cells results in a total loss of microvilli and Ezrin apical localization. These results indicate that Myo9b is a critical regulator of epithelial cell morphology and microvilli. Further, our data establish that the S1011A disease variant disrupts microvilli in human cells, suggesting a potential mechanistic link to its involvement in disease states.
ER -