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Peer-reviewedCC BYOrganic letters

Biocatalytic Cascade from Geraniol to Enantiopure (R)-Citronellal Using an Aryl-Alcohol Oxidase and Ene Reductase.

  • Claudia Ferrer-Carbonell1
  • Christian Ascaso-Alegre1,2
  • Paula Cinca-Fernando2,3
  • Patricia Ferreira2,3
  • Juan Mangas-Sánchez4
  • Caroline E Paul1
4 affiliations
  1. 1Biocatalysis Section, Department of Biotechnology, Delft University of Technology, Van der Maasweg 9, 2629 HZDelft, Netherlands.
  2. 2Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Ciencias, Universidad de Zaragoza, 50009Zaragoza, Spain.
  3. 3Instituto de Biocomputación y Física de Sistemas Complejos, BIFI (GBsC-CSIC Joint Unit), Universidad de Zaragoza, 50018Zaragoza, Spain.
  4. 4Department of Organic and Inorganic Chemistry, IUQEM, University of Oviedo, Julián Clavería 8, 33006Oviedo, Spain.
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Abstract

(R)-Citronellal, a key intermediate for (-)-menthol and other valuable fragrance compounds, is traditionally synthesized by unsustainable chemical methods, with low selectivity. Herein, we report a one-pot enzymatic cascade converting geraniol to enantiopure (R)-citronellal using Streptomyces hiroshimensis aryl-alcohol oxidase ShAAO and ene reductase Saccharomyces cerevisiae OYE2_Y83V. A scale-up reaction at 100 mM geraniol produced 95 mg of (R)-citronellal with an enantiomeric excess of >99.8%, demonstrating a sustainable, preparative-scale, and highly selective route to chiral terpenoids.

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Cite

BibTeXRIS
BibTeX
@article{ferrercarbonell2026biocatalytic,
  title = {{Biocatalytic Cascade from Geraniol to Enantiopure (R)-Citronellal Using an Aryl-Alcohol Oxidase and Ene Reductase.}},
  author = {Claudia Ferrer-Carbonell and Christian Ascaso-Alegre and Paula Cinca-Fernando and Patricia Ferreira and Juan Mangas-Sánchez and Caroline E Paul},
  journal = {Organic letters},
  year = {2026},
  month = sep,
  doi = {10.1021/acs.orglett.6c02985},
  url = {https://europepmc.org/article/MED/42918086},
  note = {CC BY},
}
RIS
TY  - JOUR
TI  - Biocatalytic Cascade from Geraniol to Enantiopure (R)-Citronellal Using an Aryl-Alcohol Oxidase and Ene Reductase.
AU  - Claudia Ferrer-Carbonell
AU  - Christian Ascaso-Alegre
AU  - Paula Cinca-Fernando
AU  - Patricia Ferreira
AU  - Juan Mangas-Sánchez
AU  - Caroline E Paul
PY  - 2026
DA  - 2026/09/01
JO  - Organic letters
DO  - 10.1021/acs.orglett.6c02985
UR  - https://europepmc.org/article/MED/42918086
AB  - (R)-Citronellal, a key intermediate for (-)-menthol and other valuable fragrance compounds, is traditionally synthesized by unsustainable chemical methods, with low selectivity. Herein, we report a one-pot enzymatic cascade converting geraniol to enantiopure (R)-citronellal using Streptomyces hiroshimensis aryl-alcohol oxidase ShAAO and ene reductase Saccharomyces cerevisiae OYE2_Y83V. A scale-up reaction at 100 mM geraniol produced 95 mg of (R)-citronellal with an enantiomeric excess of >99.8%, demonstrating a sustainable, preparative-scale, and highly selective route to chiral terpenoids.
SN  - 1523-7060
SN  - 1523-7052
ER  -