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Peer-reviewedCC BY 4.0ACS Omega

Enzymatic Bio-Oxidation of Ethyl Hydroxyethyl Cellulose and UV-Assisted Cross-Linking with Chitosan for Enhanced Aqueous Stability in Biomaterial Production

  • Beatriz Fernanda da Silva1
  • Lidiane Meier1
  • Ismael Casagrande Bellettini1
  • Karina Cesca2
  • Ana Paula Serafini Immich2
  • Patrícia Bulegon Brondani3
  1. 1Universidade Federal de Santa Catarina , , Rua Marechal Rondon, 880 - Salto do Norte , , ,
  2. 2Centro Tecnológico , , Córrego Grande , , ,
  3. 3Universidade Federal de Santa Catarina , , Rua Roberto Sampaio Gonzaga, s/n - Trindade , , ,
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Abstract

Ethyl hydroxyethyl cellulose (EHEC) is a promising sustainable biomaterial, but its high hydrophilicity limits its use in moisture-resistant applications. Conventional cross-linking to improve aqueous resistance often requires toxic reagents, organic solvents, or high temperatures. Here, we developed a green cross-linking method by enzymatically oxidizing EHEC with laccase/TEMPO (2,2,6,6-tetramethylpiperidin-1-oxyl), followed by UV-induced cross-linking with chitosan at room temperature, yielding a robust macromolecular network. The optimal conditions (4 h oxidation, 50 wt % chitosan, 24 h cross-linking) yielded prolonged aqueous-stable films that maintained structural stability for 3–5 months in aqueous media, depending on pH. Compared to purified EHEC, the biomaterial exhibited enhanced thermal behavior, a 44% increase in elastic modulus (775.6 ± 172.8 MPa), reduced hydrophilicity (75.2 ± 0.7° contact angle, compared to 56.5 ± 0.6° from the original EHEC film), and no release of toxic compounds in cytotoxic assays with L929 fibroblast cells. This methodology is tunable and efficiently produces stable, sustainable EHEC-based films, demonstrating significant promise for advanced aqueous applications and active-ingredient encapsulation.

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Cite

BibTeXRIS
BibTeX
@article{silva2026enzymatic,
  title = {{Enzymatic Bio-Oxidation of Ethyl Hydroxyethyl Cellulose and UV-Assisted Cross-Linking with Chitosan for Enhanced Aqueous Stability in Biomaterial Production}},
  author = {Beatriz Fernanda da Silva and Lidiane Meier and Ismael Casagrande Bellettini and Karina Cesca and Ana Paula Serafini Immich and Patrícia Bulegon Brondani},
  journal = {ACS Omega},
  year = {2026},
  month = oct,
  doi = {10.1021/acsomega.6c08601},
  url = {https://pubs.acs.org/acsodf/article/doi/10.1021/acsomega.6c08601/5454045/Enzymatic-Bio-Oxidation-of-Ethyl-Hydroxyethyl},
  note = {CC BY 4.0},
}
RIS
TY  - JOUR
TI  - Enzymatic Bio-Oxidation of Ethyl Hydroxyethyl Cellulose and UV-Assisted Cross-Linking with Chitosan for Enhanced Aqueous Stability in Biomaterial Production
AU  - Beatriz Fernanda da Silva
AU  - Lidiane Meier
AU  - Ismael Casagrande Bellettini
AU  - Karina Cesca
AU  - Ana Paula Serafini Immich
AU  - Patrícia Bulegon Brondani
PY  - 2026
DA  - 2026/10/20
JO  - ACS Omega
DO  - 10.1021/acsomega.6c08601
UR  - https://pubs.acs.org/acsodf/article/doi/10.1021/acsomega.6c08601/5454045/Enzymatic-Bio-Oxidation-of-Ethyl-Hydroxyethyl
AB  - Ethyl hydroxyethyl cellulose (EHEC) is a promising sustainable biomaterial, but its high hydrophilicity limits its use in moisture-resistant applications. Conventional cross-linking to improve aqueous resistance often requires toxic reagents, organic solvents, or high temperatures. Here, we developed a green cross-linking method by enzymatically oxidizing EHEC with laccase/TEMPO (2,2,6,6-tetramethylpiperidin-1-oxyl), followed by UV-induced cross-linking with chitosan at room temperature, yielding a robust macromolecular network. The optimal conditions (4 h oxidation, 50 wt % chitosan, 24 h cross-linking) yielded prolonged aqueous-stable films that maintained structural stability for 3–5 months in aqueous media, depending on pH. Compared to purified EHEC, the biomaterial exhibited enhanced thermal behavior, a 44% increase in elastic modulus (775.6 ± 172.8 MPa), reduced hydrophilicity (75.2 ± 0.7° contact angle, compared to 56.5 ± 0.6° from the original EHEC film), and no release of toxic compounds in cytotoxic assays with L929 fibroblast cells. This methodology is tunable and efficiently produces stable, sustainable EHEC-based films, demonstrating significant promise for advanced aqueous applications and active-ingredient encapsulation.
SN  - 2470-1343
ER  -