Effects of cell size on incidence of chromosome aneuploidy: Analysis using a cultured cell model.
8 affiliations
- 1Division of Molecular Genetics, Center for Medical Science, Fujita Health University, Toyoake, Aichi, Japan.
- 2Office of Research Administration, Fujita Health University, Toyoake, Aichi, Japan.
- 3Department of Biochemistry, Tohoku Medical and Pharmaceutical University, Sendai, Miyagi, Japan.
- 4Biomedical Research Support Center, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Nagasaki, Japan.
- 5Department of Biomedical Data Science, School of Medicine, Fujita Health University, Toyoake, Aichi, Japan.
- 6Laboratory of Data Management and Analysis, Open Facility Center, Fujita Health University, Toyoake, Aichi, Japan.
- 7Department of Obstetrics and Gynecology, Kansai Medical University, Hirakata, Osaka, Japan.
- 8Clinical Genetics Center, Kansai Medical University Hospital, Hirakata, Osaka, Japan.
Abstract
Objectives
Chromosomal aneuploidy arises from errors in chromosome segregation during cell division, and embryonic aneuploidy is a major cause of implantation failure and miscarriage, which is primarily attributed to maternal meiotic errors. However, recent data accumulated from preimplantation genetic testing have suggested that chromosome segregation errors may also frequently occur during mitotic divisions in early embryos. One proposed factor for these errors is that the cell size in early embryos is larger than that of somatic cells after cleavage, but the exact mechanisms remain unclear.Methods
To determine the effect of cell size on the incidence of aneuploidy, we established a cultured cell model in which the cell size was artificially enlarged. This model harbored a chromosome carrying the GFP gene, and disappearance of GFP fluorescence served as a quantitative marker for aneuploidy events. By overexpressing genes that increase cell size, we measured the cell size and frequency of aneuploidy using flow cytometry.Results
We observed a significant increase (p=3.67×10⁻⁹) in the incidence of aneuploidy in large cells, which supports the hypothesis that a large cell size characteristic of early embryos contributes to the occurrence of chromosomal segregation errors.Conclusions
Our finding may partly explain the high prevalence of aneuploid mosaicism in human preimplantation embryos.
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BibTeX
@article{kato2026effects,
title = {{Effects of cell size on incidence of chromosome aneuploidy: Analysis using a cultured cell model.}},
author = {Yoshimi Kato and Makiko Tsutsumi and Takuya Abe and Kazuo Yamamoto and Junichiro Yoshimoto and Takayuki Kannon and Hiroki Kurahashi},
journal = {Fujita medical journal},
year = {2026},
month = sep,
doi = {10.20407/fmj.2026-004},
url = {https://europepmc.org/article/MED/42968868},
note = {CC BY},
}
RIS
TY - JOUR
TI - Effects of cell size on incidence of chromosome aneuploidy: Analysis using a cultured cell model.
AU - Yoshimi Kato
AU - Makiko Tsutsumi
AU - Takuya Abe
AU - Kazuo Yamamoto
AU - Junichiro Yoshimoto
AU - Takayuki Kannon
AU - Hiroki Kurahashi
PY - 2026
DA - 2026/09/25
JO - Fujita medical journal
DO - 10.20407/fmj.2026-004
UR - https://europepmc.org/article/MED/42968868
AB - Objectives Chromosomal aneuploidy arises from errors in chromosome segregation during cell division, and embryonic aneuploidy is a major cause of implantation failure and miscarriage, which is primarily attributed to maternal meiotic errors. However, recent data accumulated from preimplantation genetic testing have suggested that chromosome segregation errors may also frequently occur during mitotic divisions in early embryos. One proposed factor for these errors is that the cell size in early embryos is larger than that of somatic cells after cleavage, but the exact mechanisms remain unclear.Methods To determine the effect of cell size on the incidence of aneuploidy, we established a cultured cell model in which the cell size was artificially enlarged. This model harbored a chromosome carrying the GFP gene, and disappearance of GFP fluorescence served as a quantitative marker for aneuploidy events. By overexpressing genes that increase cell size, we measured the cell size and frequency of aneuploidy using flow cytometry.Results We observed a significant increase (p=3.67×10⁻⁹) in the incidence of aneuploidy in large cells, which supports the hypothesis that a large cell size characteristic of early embryos contributes to the occurrence of chromosomal segregation errors.Conclusions Our finding may partly explain the high prevalence of aneuploid mosaicism in human preimplantation embryos.
SN - 2189-7247
SN - 2189-7255
ER -