Colonization, growth-promoting effect, control efficacy against tobacco black shank, and mechanism of action of the tobacco endophyte Microbacterium foliorum 5SY32
- 1College of Horticulture and Plant Protection, Henan University of Science and Technology
- 2Luoyang Branch of Henan Provincial Tobacco Company
Abstract
Tobacco black shank (TBS) severely restricts sustainable tobacco cultivation. Endophytic bacteria serve as promising biocontrol agents due to strong host colonization ability. Microbacterium foliorum 5SY32, isolated from healthy tobacco, inhibits Phytophthora nicotianae growth by over 60% and synthesizes 15.13 μg/mL indole-3-acetic acid. This study assessed its colonization, growth-promoting capability and disease suppression efficacy via greenhouse and field trials, and explored functional mechanisms through transcriptome sequencing. Re-isolation and molecular verification proved that strain 5SY32 stably colonizes tobacco roots, stems, and leaves, with the highest population detected in roots. The strain significantly optimized root morphology and physiological activity, and improved aboveground agronomic traits, leaf chlorophyll and nitrogen content. Field yield was elevated by more than 20%. It achieved 58.58% protective and 30.31% curative efficacy in the greenhouse, and sustained 48.99%−52.31% control efficacy in the field against TBS. 5SY32 boosted antioxidant and defense enzyme activities, and activated key genes related to jasmonic acid, ethylene and abscisic acid pathways. A total of 12,485 differentially expressed genes participating in cell wall formation, oxidative response and secondary metabolism were modulated, ultimately inducing systemic resistance in tobacco. This study first clarifies the integrated mechanism of colonization, growth promotion and disease resistance induction mediated by M. foliorum 5SY32, offering a valuable microbial resource for green management of TBS.
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BibTeX
@article{zheng2026colonization,
title = {{Colonization, growth-promoting effect, control efficacy against tobacco black shank, and mechanism of action of the tobacco endophyte Microbacterium foliorum 5SY32}},
author = {Wei Zheng and Haibang Du and Dongling Wu and Jianqiang Xu and Yebin Kang},
journal = {Frontiers in Microbiology},
year = {2026},
month = oct,
doi = {10.3389/fmicb.2026.1887697},
url = {https://www.frontiersin.org/articles/10.3389/fmicb.2026.1887697/full},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Colonization, growth-promoting effect, control efficacy against tobacco black shank, and mechanism of action of the tobacco endophyte Microbacterium foliorum 5SY32
AU - Wei Zheng
AU - Haibang Du
AU - Dongling Wu
AU - Jianqiang Xu
AU - Yebin Kang
PY - 2026
DA - 2026/10/08
JO - Frontiers in Microbiology
DO - 10.3389/fmicb.2026.1887697
UR - https://www.frontiersin.org/articles/10.3389/fmicb.2026.1887697/full
AB - Tobacco black shank (TBS) severely restricts sustainable tobacco cultivation. Endophytic bacteria serve as promising biocontrol agents due to strong host colonization ability. Microbacterium foliorum 5SY32, isolated from healthy tobacco, inhibits Phytophthora nicotianae growth by over 60% and synthesizes 15.13 μg/mL indole-3-acetic acid. This study assessed its colonization, growth-promoting capability and disease suppression efficacy via greenhouse and field trials, and explored functional mechanisms through transcriptome sequencing. Re-isolation and molecular verification proved that strain 5SY32 stably colonizes tobacco roots, stems, and leaves, with the highest population detected in roots. The strain significantly optimized root morphology and physiological activity, and improved aboveground agronomic traits, leaf chlorophyll and nitrogen content. Field yield was elevated by more than 20%. It achieved 58.58% protective and 30.31% curative efficacy in the greenhouse, and sustained 48.99%−52.31% control efficacy in the field against TBS. 5SY32 boosted antioxidant and defense enzyme activities, and activated key genes related to jasmonic acid, ethylene and abscisic acid pathways. A total of 12,485 differentially expressed genes participating in cell wall formation, oxidative response and secondary metabolism were modulated, ultimately inducing systemic resistance in tobacco. This study first clarifies the integrated mechanism of colonization, growth promotion and disease resistance induction mediated by M. foliorum 5SY32, offering a valuable microbial resource for green management of TBS.
SN - 1664-302X
ER -