The ABA-responsive transcription factor SmMYB32 positively modulates tanshinone biosynthesis in Salvia miltiorrhiza
Abstract
Tanshinones are natural products primarily biosynthesized in the root of Salvia miltiorrhiza. Their accumulation can be promoted by abscisic acid (ABA) treatment; however, the underlying regulatory mechanisms remain largely unclear. Here, an ABA-responsive R2R3-MYB transcription factor SmMYB32 was identified from the ABA-induced transcriptome. Overexpression of SmMYB32 significantly increased the tanshinone content in transgenic S. miltiorrhiza hairy roots by upregulating the expression of tanshinone-biosynthesis genes SmCYP76AH3 and SmCYP71D411, whereas RNA interference-mediated silencing of SmMYB32 produced the opposite effect. Further analysis using yeast one-hybrid, dual-luciferase reporter, and electrophoretic mobility shift assays demonstrated that SmMYB32 directly activates the transcription of SmCYP76AH3 and SmCYP71D411. Furthermore, SmMYB32 physically interacts with the ABA receptor SmPYL8, and this interaction inhibits the transcriptional activity of SmCYP76AH3 and SmCYP71D411. These findings uncover a novel regulatory mechanism underlying tanshinone biosynthesis and provide new insights into metabolic engineering in S. miltiorrhiza.
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BibTeX
@article{xu2026abaresponsive,
title = {{The ABA-responsive transcription factor SmMYB32 positively modulates tanshinone biosynthesis in Salvia miltiorrhiza}},
author = {Ying Xu and Ruiyan Zhu and Qinzhe Lin and Chenge Yu and Siwei Zhang and Jiaochen Qi and Jie Sun and Tingyao Liu and Qikai Huang and Lili Shao and Miaomiao Sheng and Yao Wang and Min Shi and Guoyin Kai},
journal = {Horticulture Research},
year = {2026},
month = nov,
doi = {10.1093/hr/uhag186},
url = {https://doi.org/10.1093/hr/uhag186},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - The ABA-responsive transcription factor SmMYB32 positively modulates tanshinone biosynthesis in Salvia miltiorrhiza
AU - Ying Xu
AU - Ruiyan Zhu
AU - Qinzhe Lin
AU - Chenge Yu
AU - Siwei Zhang
AU - Jiaochen Qi
AU - Jie Sun
AU - Tingyao Liu
AU - Qikai Huang
AU - Lili Shao
AU - Miaomiao Sheng
AU - Yao Wang
AU - Min Shi
AU - Guoyin Kai
PY - 2026
DA - 2026/11/04
JO - Horticulture Research
DO - 10.1093/hr/uhag186
UR - https://doi.org/10.1093/hr/uhag186
AB - Tanshinones are natural products primarily biosynthesized in the root of Salvia miltiorrhiza. Their accumulation can be promoted by abscisic acid (ABA) treatment; however, the underlying regulatory mechanisms remain largely unclear. Here, an ABA-responsive R2R3-MYB transcription factor SmMYB32 was identified from the ABA-induced transcriptome. Overexpression of SmMYB32 significantly increased the tanshinone content in transgenic S. miltiorrhiza hairy roots by upregulating the expression of tanshinone-biosynthesis genes SmCYP76AH3 and SmCYP71D411, whereas RNA interference-mediated silencing of SmMYB32 produced the opposite effect. Further analysis using yeast one-hybrid, dual-luciferase reporter, and electrophoretic mobility shift assays demonstrated that SmMYB32 directly activates the transcription of SmCYP76AH3 and SmCYP71D411. Furthermore, SmMYB32 physically interacts with the ABA receptor SmPYL8, and this interaction inhibits the transcriptional activity of SmCYP76AH3 and SmCYP71D411. These findings uncover a novel regulatory mechanism underlying tanshinone biosynthesis and provide new insights into metabolic engineering in S. miltiorrhiza.
SN - 2662-6810
SN - 2052-7276
ER -