A Newly Established Fin Cell Line from Tomato Hind (Cephalopholis sonnerati) and Transcriptome Profiling Characterizing Its Response to Vibrio harveyi Infection.
- 1Guangdong Provincial Key Laboratory for Aquatic Economic Animals, Institute of Aquatic Economic Animals, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China.
- 2State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.
- 3Hainan Fisheries Innovation Research Institute, Chinese Academy of Fishery Sciences, Sanya 572024, China.
Abstract
The tomato hind (Cephalopholis sonnerati) is an emerging mariculture species increasingly threatened by Vibrio harveyi infection, yet in vitro tools for studying its immune defense remain limited. Here, we established a novel fin-derived fibroblast-like cell line, designated Tomato hind Grouper Fin (TGF), from C. sonnerati using the tissue explant method. TGF cells have been stably subcultured for more than 83 passages in Leibovitz's L-15 medium supplemented with 20% fetal bovine serum at 28 °C. Species authentication via mt-co1 barcoding confirmed 99.9% sequence identity, and karyotype analysis revealed a diploid chromosome number of 2n = 48, matching the donor species. TGF cells exhibited robust post-thaw viability (>80%) and were amenable to exogenous nucleic acid delivery, with higher efficiency for siRNA than plasmid DNA. To evaluate immune competence, TGF cells were challenged with V. harveyi, and transcriptomic profiling was performed at 60, 90, and 120 min post-infection. A total of 1876 differentially expressed genes were identified at 120 min. KEGG enrichment analysis revealed sustained activation of Toll-like receptor, NOD-like receptor, NF-κB, TNF, and IL-17 signaling pathways across all time points. The TGF cell line represents a valuable in vitro resource for studying host-pathogen interactions of C. sonnerati.
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BibTeX
@article{wang2026newly,
title = {{A Newly Established Fin Cell Line from Tomato Hind (Cephalopholis sonnerati) and Transcriptome Profiling Characterizing Its Response to Vibrio harveyi Infection.}},
author = {Chongwei Wang and Chaowei Li and Youfeng Cai and Fei Fang and Qiaomu Hu and Lihong Zhang and Yong Zhang and Songlin Chen},
journal = {Animals : an open access journal from MDPI},
year = {2026},
month = oct,
doi = {10.3390/ani16203295},
url = {https://europepmc.org/article/MED/42939226},
note = {CC BY},
}
RIS
TY - JOUR
TI - A Newly Established Fin Cell Line from Tomato Hind (Cephalopholis sonnerati) and Transcriptome Profiling Characterizing Its Response to Vibrio harveyi Infection.
AU - Chongwei Wang
AU - Chaowei Li
AU - Youfeng Cai
AU - Fei Fang
AU - Qiaomu Hu
AU - Lihong Zhang
AU - Yong Zhang
AU - Songlin Chen
PY - 2026
DA - 2026/10/20
JO - Animals : an open access journal from MDPI
DO - 10.3390/ani16203295
UR - https://europepmc.org/article/MED/42939226
AB - The tomato hind (Cephalopholis sonnerati) is an emerging mariculture species increasingly threatened by Vibrio harveyi infection, yet in vitro tools for studying its immune defense remain limited. Here, we established a novel fin-derived fibroblast-like cell line, designated Tomato hind Grouper Fin (TGF), from C. sonnerati using the tissue explant method. TGF cells have been stably subcultured for more than 83 passages in Leibovitz's L-15 medium supplemented with 20% fetal bovine serum at 28 °C. Species authentication via mt-co1 barcoding confirmed 99.9% sequence identity, and karyotype analysis revealed a diploid chromosome number of 2n = 48, matching the donor species. TGF cells exhibited robust post-thaw viability (>80%) and were amenable to exogenous nucleic acid delivery, with higher efficiency for siRNA than plasmid DNA. To evaluate immune competence, TGF cells were challenged with V. harveyi, and transcriptomic profiling was performed at 60, 90, and 120 min post-infection. A total of 1876 differentially expressed genes were identified at 120 min. KEGG enrichment analysis revealed sustained activation of Toll-like receptor, NOD-like receptor, NF-κB, TNF, and IL-17 signaling pathways across all time points. The TGF cell line represents a valuable in vitro resource for studying host-pathogen interactions of C. sonnerati.
SN - 2076-2615
ER -