The SlHYL1-SlRBP1 module integrates gibberellin metabolism to promote plant height in tomato (Solanum lycopersicum).
4 affiliations
- 1Key Laboratory of Quality and Safety Control for Subtropical Fruit and Vegetable, Ministry of Agriculture and Rural Affairs, College of Horticulture, Zhejiang A&F University, Hangzhou, 311300, China.
- 2College of Life Sciences, Zaozhuang University, Zaozhuang, 277160, China.
- 3College of Advanced Agricultural Sciences, Zhejiang Wanli University, Ningbo, 315100, China.
- 4State Key Laboratory of Genetics and Development of Complex Phenotypes, Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai, 200438, China.
Abstract
Plant height is an essential agronomic trait that significantly influences crop yield and quality. The HYPONASTIC LEAVES 1 (HYL1), a core component of microRNA biogenesis, remains poorly understood in terms of its regulatory mechanisms on agronomic traits in tomato (Solanum lycopersicum, Sl). In this study, we demonstrated that CRISPR/Cas9-mediated knockout of SlHYL1 caused dwarfism, and partial suppression of SlHYL1 in tomato cultivar "Ailsa Craig" (AC) via RNA interference (RNAi) increased internode number but reduced internode length, resulting in a semi-dwarf phenotype, confirming the role of SlHYL1 in regulating plant height. Cytological analysis showed that SlHYL1 suppression decreased both cell number and size within internodes. Yeast two-hybrid screening identified that SlHYL1 interacted with another tomato RNA-binding protein 1 (SlRBP1). Knockdown of SlRBP1 resembled the SlHYL1-RNAi semi-dwarf phenotype. Further studies revealed that the SlHYL1-SlRBP1 module additively promotes the expression of gibberellin (GA) biosynthetic genes, thereby facilitating GA accumulation. Additionally, the dwarf phenotypes of both SlHYL1-RNAi and SlRBP1-RNAi plants were partially rescued by exogenous GA₃ application. Notably, unlike SlHYL1, SlRBP1 did not affect the biogenesis of most miRNAs. In summary, our findings demonstrate that the SlHYL1-SlRBP1 module regulates tomato plant height by enhancing GA accumulation.
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BibTeX
@article{zhang2026slhyl1slrbp1,
title = {{The SlHYL1-SlRBP1 module integrates gibberellin metabolism to promote plant height in tomato (Solanum lycopersicum).}},
author = {Xingxing Zhang and Miao Chai and Linjing Cao and Shengbo Chen and Kun Zhao and Jiazhen Han and Ning Li and Hongjiao Zhao and Shengjun Feng and Bo Wang and Shengze Zhang and Yumeng Li and Mingze Xu and Guodong Ren and Shuai Gao},
journal = {The Plant journal : for cell and molecular biology},
year = {2026},
month = nov,
doi = {10.1111/tpj.71210},
url = {https://europepmc.org/article/MED/42968607},
note = {CC BY},
}
RIS
TY - JOUR
TI - The SlHYL1-SlRBP1 module integrates gibberellin metabolism to promote plant height in tomato (Solanum lycopersicum).
AU - Xingxing Zhang
AU - Miao Chai
AU - Linjing Cao
AU - Shengbo Chen
AU - Kun Zhao
AU - Jiazhen Han
AU - Ning Li
AU - Hongjiao Zhao
AU - Shengjun Feng
AU - Bo Wang
AU - Shengze Zhang
AU - Yumeng Li
AU - Mingze Xu
AU - Guodong Ren
AU - Shuai Gao
PY - 2026
DA - 2026/11/01
JO - The Plant journal : for cell and molecular biology
DO - 10.1111/tpj.71210
UR - https://europepmc.org/article/MED/42968607
AB - Plant height is an essential agronomic trait that significantly influences crop yield and quality. The HYPONASTIC LEAVES 1 (HYL1), a core component of microRNA biogenesis, remains poorly understood in terms of its regulatory mechanisms on agronomic traits in tomato (Solanum lycopersicum, Sl). In this study, we demonstrated that CRISPR/Cas9-mediated knockout of SlHYL1 caused dwarfism, and partial suppression of SlHYL1 in tomato cultivar "Ailsa Craig" (AC) via RNA interference (RNAi) increased internode number but reduced internode length, resulting in a semi-dwarf phenotype, confirming the role of SlHYL1 in regulating plant height. Cytological analysis showed that SlHYL1 suppression decreased both cell number and size within internodes. Yeast two-hybrid screening identified that SlHYL1 interacted with another tomato RNA-binding protein 1 (SlRBP1). Knockdown of SlRBP1 resembled the SlHYL1-RNAi semi-dwarf phenotype. Further studies revealed that the SlHYL1-SlRBP1 module additively promotes the expression of gibberellin (GA) biosynthetic genes, thereby facilitating GA accumulation. Additionally, the dwarf phenotypes of both SlHYL1-RNAi and SlRBP1-RNAi plants were partially rescued by exogenous GA₃ application. Notably, unlike SlHYL1, SlRBP1 did not affect the biogenesis of most miRNAs. In summary, our findings demonstrate that the SlHYL1-SlRBP1 module regulates tomato plant height by enhancing GA accumulation.
SN - 0960-7412
SN - 1365-313X
ER -