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Peer-reviewedCC BY-NC-ND 4.0Poultry Science

A sensitive RAA-CRISPR/Cas13a-LFS assay for point-of-care detection of Salmonella

  • Xiaoliang Xiao1,2
  • Wenzhe Liu1
  • Di Lei1
  • Chenchen Jiang1
  • Tong Li1
  • Wenxin Luo1
  • Xin Wang1
  • Yi Tang3
  • Rendong Fang1
  • Hongzhi Wang1
  1. 1College of Veterinary Medicine, Southwest University, Beibei District, Chongqing, China
  2. 2Library, Shandong Agricultural University, Tai’an, Shandong, China
  3. 3Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Haidian District, Beijing, China
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Abstract

Salmonella is a major zoonotic pathogen that seriously endangers the poultry industry and threatens public health. Rapid and accurate monitoring is therefore essential for effective prevention and control. In this study, we developed a molecular detection method for Salmonella by integrating recombinase-assisted amplification (RAA) and the CRISPR/Cas13a system. We then adapted this method into a lateral flow strip (LFS) format that allows naked‑eye reading, enabling instrument‑free, rapid on‑site detection. The assay combines the efficient isothermal amplification ability of RAA with the high specificity recognition of CRISPR/Cas13a, targeting the conserved fimY gene of Salmonella. It achieves a detection sensitivity of 10° copies/μL. When further coupled with LFS for visual detection, the reaction can be finished within 40 min, with a detection limit of 102 copies/μL. In clinical sample validation, the method showed 100% agreement with qPCR results and significantly outperformed conventional PCR. This assay provides a sensitive and specific approach for Salmonella detection that is simple to operate and suitable for field deployment, offering a practical tool for rapid Salmonella screening in resource-limited settings.

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Cite

BibTeXRIS
BibTeX
@article{xiao2026sensitive,
  title = {{A sensitive RAA-CRISPR/Cas13a-LFS assay for point-of-care detection of Salmonella}},
  author = {Xiaoliang Xiao and Wenzhe Liu and Di Lei and Chenchen Jiang and Tong Li and Wenxin Luo and Xin Wang and Yi Tang and Rendong Fang and Hongzhi Wang},
  journal = {Poultry Science},
  year = {2026},
  month = oct,
  doi = {10.1016/j.psj.2026.107962},
  url = {https://doi.org/10.1016/j.psj.2026.107962},
  note = {CC BY-NC-ND 4.0},
}
RIS
TY  - JOUR
TI  - A sensitive RAA-CRISPR/Cas13a-LFS assay for point-of-care detection of Salmonella
AU  - Xiaoliang Xiao
AU  - Wenzhe Liu
AU  - Di Lei
AU  - Chenchen Jiang
AU  - Tong Li
AU  - Wenxin Luo
AU  - Xin Wang
AU  - Yi Tang
AU  - Rendong Fang
AU  - Hongzhi Wang
PY  - 2026
DA  - 2026/10/14
JO  - Poultry Science
DO  - 10.1016/j.psj.2026.107962
UR  - https://doi.org/10.1016/j.psj.2026.107962
AB  - Salmonella is a major zoonotic pathogen that seriously endangers the poultry industry and threatens public health. Rapid and accurate monitoring is therefore essential for effective prevention and control. In this study, we developed a molecular detection method for Salmonella by integrating recombinase-assisted amplification (RAA) and the CRISPR/Cas13a system. We then adapted this method into a lateral flow strip (LFS) format that allows naked‑eye reading, enabling instrument‑free, rapid on‑site detection. The assay combines the efficient isothermal amplification ability of RAA with the high specificity recognition of CRISPR/Cas13a, targeting the conserved fimY gene of Salmonella. It achieves a detection sensitivity of 10° copies/μL. When further coupled with LFS for visual detection, the reaction can be finished within 40 min, with a detection limit of 102 copies/μL. In clinical sample validation, the method showed 100% agreement with qPCR results and significantly outperformed conventional PCR. This assay provides a sensitive and specific approach for Salmonella detection that is simple to operate and suitable for field deployment, offering a practical tool for rapid Salmonella screening in resource-limited settings.
SN  - 0032-5791
SN  - 1525-3171
ER  -