Cross-Disease Transcriptomic Signatures Shared by NAFLD and MI: Multicohort Machine-Learning Analysis and Exploratory IDI1 Expression Assessment
- 1Department of Cardiology, First Affiliated Hospital of Anhui Medical University, Hefei 230032, China
- 2Department of General Surgery, First Affiliated Hospital of Anhui Medical University, Hefei 230032, China
- 3The First Clinical College, Anhui Medical University, Hefei 230022, China
Abstract
The molecular features shared by non-alcoholic fatty liver disease (NAFLD) and myocardial infarction (MI) remain incompletely understood. We analyzed six microarray cohorts from the GEO database to identify shared disease-associated transcriptomic features across independent NAFLD and MI populations. Weighted gene co-expression network analysis, differential expression analysis, and fuzzy c-means clustering across ordered cross-sectional NAFLD groups were used to define candidate genes. LASSO regression, random forest, and SVM-RFE identified consensus computational candidates, which were examined using exploratory classification, receiver operating characteristic curves, calibration curves, decision curve analysis, and SHapley Additive exPlanations. IDI1 was selected for exploratory expression assessment by qPCR in patients with established MI and in a mouse model of acute ischemic myocardial injury. Fifty-three shared candidate genes were identified, and five genes were retained by all three feature-selection methods. Single-gene ROC analyses showed SULF2, GADD45A, LIN7A, and IDI1 within the analyzed case-control datasets. IDI1 expression was higher in patients with MI than in healthy controls (p = 0.004), as well as in mice blood (p = 0.034) and infarcted myocardium (p = 0.0072) following LAD ligation. These findings indicate shared transcriptomic links between NAFLD and MI, supporting an association between IDI1 and myocardial injury.
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BibTeX
@article{qiao2026crossdisease,
title = {{Cross-Disease Transcriptomic Signatures Shared by NAFLD and MI: Multicohort Machine-Learning Analysis and Exploratory IDI1 Expression Assessment}},
author = {Jilei Qiao and Shuran Chen and Shuo Li and Wenbo Shao and Yiran Zhang and Ying Huang and Yangjing Xie},
journal = {International Journal of Molecular Sciences},
year = {2026},
month = oct,
doi = {10.3390/ijms27209390},
url = {https://www.mdpi.com/1422-0067/27/20/9390},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Cross-Disease Transcriptomic Signatures Shared by NAFLD and MI: Multicohort Machine-Learning Analysis and Exploratory IDI1 Expression Assessment
AU - Jilei Qiao
AU - Shuran Chen
AU - Shuo Li
AU - Wenbo Shao
AU - Yiran Zhang
AU - Ying Huang
AU - Yangjing Xie
PY - 2026
DA - 2026/10/01
JO - International Journal of Molecular Sciences
DO - 10.3390/ijms27209390
UR - https://www.mdpi.com/1422-0067/27/20/9390
AB - The molecular features shared by non-alcoholic fatty liver disease (NAFLD) and myocardial infarction (MI) remain incompletely understood. We analyzed six microarray cohorts from the GEO database to identify shared disease-associated transcriptomic features across independent NAFLD and MI populations. Weighted gene co-expression network analysis, differential expression analysis, and fuzzy c-means clustering across ordered cross-sectional NAFLD groups were used to define candidate genes. LASSO regression, random forest, and SVM-RFE identified consensus computational candidates, which were examined using exploratory classification, receiver operating characteristic curves, calibration curves, decision curve analysis, and SHapley Additive exPlanations. IDI1 was selected for exploratory expression assessment by qPCR in patients with established MI and in a mouse model of acute ischemic myocardial injury. Fifty-three shared candidate genes were identified, and five genes were retained by all three feature-selection methods. Single-gene ROC analyses showed SULF2, GADD45A, LIN7A, and IDI1 within the analyzed case-control datasets. IDI1 expression was higher in patients with MI than in healthy controls (p = 0.004), as well as in mice blood (p = 0.034) and infarcted myocardium (p = 0.0072) following LAD ligation. These findings indicate shared transcriptomic links between NAFLD and MI, supporting an association between IDI1 and myocardial injury.
SN - 1422-0067
ER -