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Peer-reviewedCC BYJournal of ophthalmic & vision research

NanoskinⓇ Bacterial Cellulose as a Scaffold for De-epithelialized Corneal Surface Healing.

  • Magda Viveiros1
  • Cláudia Rainho2
  • Natália Avelino2
  • Carolina Gorgulho2
  • Ramon Kaneno2
  • Alexandre Fabro3
  • Márcio Viveiros4
  • Pierre Basmaji5
  • Nick Di Girolamo6
  • Silvana Schellini1
6 affiliations
  1. 1Department of Ophthalmology, Medical School, São Paulo State University (Unesp), Botucatu, São Paulo, Brazil.
  2. 2Department of Chemical and Biological Sciences, Institute of Biosciences, São Paulo State University (Unesp), Botucatu, São Paulo, Brazil.
  3. 3Department of Pathology, University of Sao Paulo - USP, Ribeirao Preto, SP, Brazil.
  4. 4Department of Orthopedics and Traumatology, Medical School, São Paulo State University (Unesp), Botucatu, São Paulo, Brazil.
  5. 5Innovatecs Biotechnological Research and Development, São Carlos, SP, Brazil.
  6. 6Department of Ophthalmology, University of New South Wales, Sydney, Australia.
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Abstract

Purpose

This study investigates the ability of Nanoskin, a bacterial cellulose (BC) biofilm, to transfer human corneal epithelial cells (CECs) onto wounded rabbit corneas, evaluating integration and re-epithelialization of the ocular surface.Methods

Primary cultures of human mesenchymal stem cells (MSCs) were established and differentiated into CECs, which were then seeded onto Nanoskin BC or soft contact lenses. Wounded rabbit corneas were covered with human CECs on BC biofilms or contact lenses and maintained in organ culture for 5 days. The control group was left untreated. Integration and re-epithelialization were assessed using immunofluorescence (IF) staining with human anti-nuclei antibodies.Results

Wounded rabbit corneas successfully re-epithelialized with both scaffolds. IF staining confirmed the presence of human epithelial cells in the regenerating rabbit corneal epithelium for both Nanoskin BC and contact lens groups, confirming effective cell transfer. Morphometric analysis revealed that control wounded corneas had significantly fewer epithelial cells (P < 0.001) compared to those treated with cell transfer.Conclusion

Our findings demonstrate the feasibility of using Nanoskin BC as a scaffold for corneal cell transplantation, highlighting its potential as a promising biomaterial for treating ocular surface diseases. Future investigations are required to evaluate the transfer efficiency of MSCs, given their superior proliferative capacity and secretion of growth factors.

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Cite

BibTeXRIS
BibTeX
@article{viveiros2026nanoskinⓡ,
  title = {{NanoskinⓇ Bacterial Cellulose as a Scaffold for De-epithelialized Corneal Surface Healing.}},
  author = {Magda Viveiros and Cláudia Rainho and Natália Avelino and Carolina Gorgulho and Ramon Kaneno and Alexandre Fabro and Márcio Viveiros and Pierre Basmaji and Nick Di Girolamo and Silvana Schellini},
  journal = {Journal of ophthalmic \& vision research},
  year = {2026},
  month = oct,
  doi = {10.18502/jovr.v21.18451},
  url = {https://europepmc.org/article/MED/42948073},
  note = {CC BY},
}
RIS
TY  - JOUR
TI  - NanoskinⓇ Bacterial Cellulose as a Scaffold for De-epithelialized Corneal Surface Healing.
AU  - Magda Viveiros
AU  - Cláudia Rainho
AU  - Natália Avelino
AU  - Carolina Gorgulho
AU  - Ramon Kaneno
AU  - Alexandre Fabro
AU  - Márcio Viveiros
AU  - Pierre Basmaji
AU  - Nick Di Girolamo
AU  - Silvana Schellini
PY  - 2026
DA  - 2026/10/06
JO  - Journal of ophthalmic & vision research
DO  - 10.18502/jovr.v21.18451
UR  - https://europepmc.org/article/MED/42948073
AB  - Purpose  This study investigates the ability of Nanoskin, a bacterial cellulose (BC) biofilm, to transfer human corneal epithelial cells (CECs) onto wounded rabbit corneas, evaluating integration and re-epithelialization of the ocular surface.Methods  Primary cultures of human mesenchymal stem cells (MSCs) were established and differentiated into CECs, which were then seeded onto Nanoskin BC or soft contact lenses. Wounded rabbit corneas were covered with human CECs on BC biofilms or contact lenses and maintained in organ culture for 5 days. The control group was left untreated. Integration and re-epithelialization were assessed using immunofluorescence (IF) staining with human anti-nuclei antibodies.Results  Wounded rabbit corneas successfully re-epithelialized with both scaffolds. IF staining confirmed the presence of human epithelial cells in the regenerating rabbit corneal epithelium for both Nanoskin BC and contact lens groups, confirming effective cell transfer. Morphometric analysis revealed that control wounded corneas had significantly fewer epithelial cells (P < 0.001) compared to those treated with cell transfer.Conclusion  Our findings demonstrate the feasibility of using Nanoskin BC as a scaffold for corneal cell transplantation, highlighting its potential as a promising biomaterial for treating ocular surface diseases. Future investigations are required to evaluate the transfer efficiency of MSCs, given their superior proliferative capacity and secretion of growth factors.
SN  - 2008-2010
SN  - 2008-322X
ER  -