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Peer-reviewedCC BY-NC-NDJournal of orthopaedic surgery and research

Expression and clinical significance of miR-34a-5p in lumbar disc herniation.

  • Boyu Song1
  • Rui Dong2
  • Tingting Liu2
  • Xu Yang2
  • Zhili Zhang2
  • Haixia Feng3
  1. 1Orthopedics Department, The First Hospital of Qinhuangdao, Qinhuangdao, 066000, China.
  2. 2Pain Management Department, The First Hospital of Qinhuangdao, No.258, Wenhua Road, Haigang District, Qinhuangdao, 066000, China.
  3. 3Pain Management Department, The First Hospital of Qinhuangdao, No.258, Wenhua Road, Haigang District, Qinhuangdao, 066000, China. [email protected].
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Abstract

Aims

This study aimed to investigate the expression, clinical significance, and underlying molecular mechanism of miR-34a-5p in Lumbar disc herniation (LDH).Methods

A total of 112 patients with LDH and 112 healthy controls were enrolled. Serum miR-34a-5p expression was measured by RT-qPCR, and its diagnostic value was assessed using receiver operating characteristic curve analysis. The correlations of miR-34a-5p with clinical indicators and inflammatory factors were analyzed. In vitro, an IL-1β-induced inflammatory injury model of human nucleus pulposus cells (HNPCs) was established to verify the function of miR-34a-5p.Results

Serum miR-34a-5p was markedly upregulated in patients with LDHmiR-34a-5p was positively correlated with disease severity, the Visual Analogue Scale, Oswestry Disability Index, Pfirrmann grade, TNF-α, IL-1β, IL-6, and negatively correlated with Japanese Orthopaedic Association score and IL-10. In vitro, IL-1β upregulated miR-34a-5p in HNPCs; miR-34a-5p overexpression aggravated the inhibition of cell viability and the inflammatory response, whereas its knockdown alleviated these effects. Mechanistically, miR-34a-5p directly targeted and suppressed ZBTB20 mRNA expression.Conclusions

miR-34a-5p is highly expressed in LDH and may serves as a promising diagnostic biomarker. It promotes HNPC inflammatory injury and LDH progression by targeting ZBTB20.

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Cite

BibTeXRIS
BibTeX
@article{song2026expression,
  title = {{Expression and clinical significance of miR-34a-5p in lumbar disc herniation.}},
  author = {Boyu Song and Rui Dong and Tingting Liu and Xu Yang and Zhili Zhang and Haixia Feng},
  journal = {Journal of orthopaedic surgery and research},
  year = {2026},
  month = jul,
  doi = {10.1186/s13018-026-07131-5},
  url = {https://europepmc.org/article/MED/42919401},
  note = {CC BY-NC-ND},
}
RIS
TY  - JOUR
TI  - Expression and clinical significance of miR-34a-5p in lumbar disc herniation.
AU  - Boyu Song
AU  - Rui Dong
AU  - Tingting Liu
AU  - Xu Yang
AU  - Zhili Zhang
AU  - Haixia Feng
PY  - 2026
DA  - 2026/07/01
JO  - Journal of orthopaedic surgery and research
DO  - 10.1186/s13018-026-07131-5
UR  - https://europepmc.org/article/MED/42919401
AB  - Aims  This study aimed to investigate the expression, clinical significance, and underlying molecular mechanism of miR-34a-5p in Lumbar disc herniation (LDH).Methods  A total of 112 patients with LDH and 112 healthy controls were enrolled. Serum miR-34a-5p expression was measured by RT-qPCR, and its diagnostic value was assessed using receiver operating characteristic curve analysis. The correlations of miR-34a-5p with clinical indicators and inflammatory factors were analyzed. In vitro, an IL-1β-induced inflammatory injury model of human nucleus pulposus cells (HNPCs) was established to verify the function of miR-34a-5p.Results  Serum miR-34a-5p was markedly upregulated in patients with LDHmiR-34a-5p was positively correlated with disease severity, the Visual Analogue Scale, Oswestry Disability Index, Pfirrmann grade, TNF-α, IL-1β, IL-6, and negatively correlated with Japanese Orthopaedic Association score and IL-10. In vitro, IL-1β upregulated miR-34a-5p in HNPCs; miR-34a-5p overexpression aggravated the inhibition of cell viability and the inflammatory response, whereas its knockdown alleviated these effects. Mechanistically, miR-34a-5p directly targeted and suppressed ZBTB20 mRNA expression.Conclusions  miR-34a-5p is highly expressed in LDH and may serves as a promising diagnostic biomarker. It promotes HNPC inflammatory injury and LDH progression by targeting ZBTB20.
SN  - 1749-799X
ER  -