Skip to content
DraconiticResearch
Search Sources API
194,161 studies31 sourcesUpdated 25 min ago
Peer-reviewedCC BY 4.0Frontiers in Pharmacology

A high-throughput 96-well plate method to measure human uterine contractility

  • Emilie Huon1
  • Justine Rayssac1
  • Marie-Charlotte Lamau2
  • Carole Abo2
  • Gauthier Dietrich2
  • Ylann Abrahami2
  • Jordan Kouby2
  • Julien Adam3
  • Nicolas R. Chevalier1
  1. 1Laboratoire Matières et Systèmes Complexes, Université Paris Cité, CNRS, Paris, France
  2. 2Service de Chirurgie Gynécologique, Hôpital Paris Saint-Joseph, Hôpital Marie Lannelongue, Paris, France
  3. 3Service d’Anatomie pathologique, Hôpitaux Paris Saint-Joseph Marie-Lannelongue, Paris, France
Read full textPDFDOICite

Abstract

Uterine smooth muscle dysfunction is a common factor in many gynecological conditions, such as endometriosis, adenomyosis, dysmenorrhea, infertility, and labor dystocia. Here, we present a novel high-throughput, optical, low-cost, 96-well plate-based method for measuring ex-vivo uterine contractility. We validate this new method on non-pregnant human myometrial samples obtained from hysterectomies by systematically comparing contraction frequency, amplitude, and duration with the standard isometric force transducer approach. We illustrate the potential of this method by screening 8 different compounds, including hormones and pharmacological agents. In particular, we found that antagonists of T-type calcium channels (Z944) and chloride channels (benzbromarone) both decreased contractility in the non-pregnant human uterus. This method will accelerate the pharmacological development of myometrium-specific relaxing agents and provide a powerful tool to investigate the dysregulation of myogenic contractility in gynecological pathologies.

Related

  1. A droplet-based long-read single-cell epigenome sequencing method for the high-throughput profiling of multidimensional regulatory elements within individual cells
    Jiansen Lu, Kexuan Chen, Shengtao Jia +3
    Cell DiscoveryPeer-reviewedCC BY 4.0Full textMolecular biology & gene regulationPMCDOAJ
  2. Transient Neonatal Uterine Bleeding Offers a Window to Understanding Uterine Development
    Marwan Habiba, Sun-Wei Guo, Giuseppe Benagiano
    BiomoleculesPeer-reviewedCC BYFull textGeneral biochemistryEurope PMC
  3. Laser phase plate improves structure determination of small proteins by cryo-EM
    Petar N. Petrov, Jessie T. Zhang, Jonathan P. Remis +8
    SciencePeer-reviewedLicence not stated9 citedStructural biologyOpenAlex
  4. Ginsenoside Rb1 alleviates uterine aging via AMPK-mediated mTOR inactivation
    Ping Zhou, Yue Wang, Ming Cheng +14
    Signal Transduction and Targeted TherapyPeer-reviewedCC BY-NC-ND 4.0Full textMolecular biology & gene regulationPMC
  5. Retinoic acid signaling in growth plate development: receptor-specific effects, antagonist evidence, and pediatric translational considerations.
    Yutong Xing, Ruiying Fan, Kaixi Wang +3
    Frontiers in endocrinologyPeer-reviewedCC BYFull textMolecular biology & gene regulationEurope PMCCrossref

Cite

BibTeXRIS
BibTeX
@article{huon2026highthroughput,
  title = {{A high-throughput 96-well plate method to measure human uterine contractility}},
  author = {Emilie Huon and Justine Rayssac and Marie-Charlotte Lamau and Carole Abo and Gauthier Dietrich and Ylann Abrahami and Jordan Kouby and Julien Adam and Nicolas R. Chevalier},
  journal = {Frontiers in Pharmacology},
  year = {2026},
  month = oct,
  doi = {10.3389/fphar.2026.1953934},
  url = {https://doi.org/10.3389/fphar.2026.1953934},
  note = {CC BY 4.0},
}
RIS
TY  - JOUR
TI  - A high-throughput 96-well plate method to measure human uterine contractility
AU  - Emilie Huon
AU  - Justine Rayssac
AU  - Marie-Charlotte Lamau
AU  - Carole Abo
AU  - Gauthier Dietrich
AU  - Ylann Abrahami
AU  - Jordan Kouby
AU  - Julien Adam
AU  - Nicolas R. Chevalier
PY  - 2026
DA  - 2026/10/12
JO  - Frontiers in Pharmacology
DO  - 10.3389/fphar.2026.1953934
UR  - https://doi.org/10.3389/fphar.2026.1953934
AB  - Uterine smooth muscle dysfunction is a common factor in many gynecological conditions, such as endometriosis, adenomyosis, dysmenorrhea, infertility, and labor dystocia. Here, we present a novel high-throughput, optical, low-cost, 96-well plate-based method for measuring ex-vivo uterine contractility. We validate this new method on non-pregnant human myometrial samples obtained from hysterectomies by systematically comparing contraction frequency, amplitude, and duration with the standard isometric force transducer approach. We illustrate the potential of this method by screening 8 different compounds, including hormones and pharmacological agents. In particular, we found that antagonists of T-type calcium channels (Z944) and chloride channels (benzbromarone) both decreased contractility in the non-pregnant human uterus. This method will accelerate the pharmacological development of myometrium-specific relaxing agents and provide a powerful tool to investigate the dysregulation of myogenic contractility in gynecological pathologies.
SN  - 1663-9812
ER  -