Endocytosis and Endocytic Neurotrophin Signaling in Developing Spiral Ganglion Neurons.
5 affiliations
- 1Department of Otolaryngology, University of California San Diego (UCSD), La Jolla, CA 92093, USA.
- 2Audiology, Mary Bridge Children's Hospital, Tacoma, WA 98405, USA.
- 3Research Department, VA San Diego Medical Center, San Diego, CA 92161, USA.
- 4Translational Neuroscience Facility, Department of Physiology, School of Biomedical Sciences, UNSW Sydney, Sydney, NSW 2052, Australia.
- 5Department of Neurosciences, University of California San Diego (UCSD), La Jolla, CA 92093, USA.
Abstract
Spiral ganglion neurons (SGNs) relay auditory information from cochlear sensory cells to the central nervous system (CNS). During development, neurotrophins (NTs) secreted by sensory and supporting cells promote SGN dendrite outgrowth. In injury models, exogenous NTs have been used to stimulate SGN regeneration after damage. Following ligand binding, NT-Trk receptor complexes are internalized and may undergo recycling or degradation, or remain intracellular as "signaling endosomes" that can be retrogradely transported to the soma to influence gene expression, survival, and neuritogenesis. Here, we investigated the role of Trk-containing endosomes in SGNs. Neonatal rat SG explants were treated with BDNF and NT-3 in combination with inhibitors targeting distinct steps of endocytosis. Neurite number and length were quantified and compared to untreated controls. Inhibition of actin polymerization (cytochalasin-B) or dynamin (Dynasore) strongly suppressed neurite outgrowth in both NT-treated and untreated explants. Similarly, inhibitors of clathrin-mediated endocytosis, including those acting upstream of dynamin, reduced neuritogenesis under both conditions. In contrast, inhibition of clathrin-independent pathways diminished neurite growth only in the absence of NTs. Blocking retrograde transport of endosomes to the trans-Golgi network (Retro-2) selectively increased neurite length in NT-treated explants, while inhibition of retrograde transport along neurites (Mycalolide B) markedly reduced both neurite number and extension regardless of NT treatment. These findings suggest that basal SGN neuritogenesis relies on both clathrin-dependent and clathrin-independent endocytosis, whereas NT-induced neurite growth is specifically mediated through clathrin- and dynamin-dependent mechanisms. Although some inhibitors also disrupt actin polymerization, the lack of effect from clathrin-independent inhibitors supports clathrin-mediated internalization as the predominant pathway for NT-driven SGN growth.
Related
- Palmitoylation of p75 neurotrophin receptor is required for retrograde regressive signaling
- Endocytosis and Compartmentalized Intracellular Signaling of the Prostaglandin Receptor EP4 Mediate Pain
- Ectopic expression of melanopsin promotes axonal regeneration in retinal ganglion cells via cAMP and TRPC signaling
- Developing synthetic auxin-based trans-kingdom signaling
- Role of Very Long-Chain Fatty Acyl-Ceramides in Endocytic Uptake
BibTeX
@article{doolittle2026endocytosis,
title = {{Endocytosis and Endocytic Neurotrophin Signaling in Developing Spiral Ganglion Neurons.}},
author = {Shannon Doolittle and Kwang Pak and Eduardo Chavez and Gary D Housley and Arwa Kurabi and Allen F Ryan},
journal = {International journal of molecular sciences},
year = {2026},
month = sep,
doi = {10.3390/ijms27198527},
url = {https://europepmc.org/article/MED/42883773},
note = {CC BY},
}
RIS
TY - JOUR
TI - Endocytosis and Endocytic Neurotrophin Signaling in Developing Spiral Ganglion Neurons.
AU - Shannon Doolittle
AU - Kwang Pak
AU - Eduardo Chavez
AU - Gary D Housley
AU - Arwa Kurabi
AU - Allen F Ryan
PY - 2026
DA - 2026/09/24
JO - International journal of molecular sciences
DO - 10.3390/ijms27198527
UR - https://europepmc.org/article/MED/42883773
AB - Spiral ganglion neurons (SGNs) relay auditory information from cochlear sensory cells to the central nervous system (CNS). During development, neurotrophins (NTs) secreted by sensory and supporting cells promote SGN dendrite outgrowth. In injury models, exogenous NTs have been used to stimulate SGN regeneration after damage. Following ligand binding, NT-Trk receptor complexes are internalized and may undergo recycling or degradation, or remain intracellular as "signaling endosomes" that can be retrogradely transported to the soma to influence gene expression, survival, and neuritogenesis. Here, we investigated the role of Trk-containing endosomes in SGNs. Neonatal rat SG explants were treated with BDNF and NT-3 in combination with inhibitors targeting distinct steps of endocytosis. Neurite number and length were quantified and compared to untreated controls. Inhibition of actin polymerization (cytochalasin-B) or dynamin (Dynasore) strongly suppressed neurite outgrowth in both NT-treated and untreated explants. Similarly, inhibitors of clathrin-mediated endocytosis, including those acting upstream of dynamin, reduced neuritogenesis under both conditions. In contrast, inhibition of clathrin-independent pathways diminished neurite growth only in the absence of NTs. Blocking retrograde transport of endosomes to the trans-Golgi network (Retro-2) selectively increased neurite length in NT-treated explants, while inhibition of retrograde transport along neurites (Mycalolide B) markedly reduced both neurite number and extension regardless of NT treatment. These findings suggest that basal SGN neuritogenesis relies on both clathrin-dependent and clathrin-independent endocytosis, whereas NT-induced neurite growth is specifically mediated through clathrin- and dynamin-dependent mechanisms. Although some inhibitors also disrupt actin polymerization, the lack of effect from clathrin-independent inhibitors supports clathrin-mediated internalization as the predominant pathway for NT-driven SGN growth.
SN - 1422-0067
ER -