Lactiplantibacillus plantarum-Fermented Broccoli Attenuates Metabolic Dysfunction and Improves Skeletal Muscle Quality in High-Fat/High-Fructose Diet-Fed Mice.
4 affiliations
- 1Institute of Food Sciences and Technology, National Taiwan University, Taipei10617, Taiwan.
- 2Research and Development Department, Syngen Biotech Co., Ltd., Tainan73055, Taiwan.
- 3Department of Medical Research, China Medical University Hospital, China Medical University, Taichung40402, Taiwan.
- 4Department of Health and Nutrition Biotechnology, Asia University, Taichung413305, Taiwan.
Abstract
Muscle loss accompanied by excessive adiposity is a critical component of metabolic dysfunction. This study investigated the effects of Lactiplantibacillus plantarum-fermented broccoli (FHB) in high-fat/high-fructose diet (HFFD)-fed C57BL/6J mice. LC-MS profiling showed that fermentation generated metabolites, including 2-hydroxycaproic acid, 3-phenyllactic acid, and a tetrahydro-β-carboline derivative. FHB supplementation attenuated visceral adiposity, hepatic steatosis, and insulin resistance while preserving grip strength. In the gastrocnemius muscle, FHB was associated with higher phosphorylation of the IRS1/PI3K/Akt and mTOR/p70S6K/4E-BP1 pathways and with lower expression of the ubiquitin ligases Atrogin-1 and MuRF-1. 16S rRNA sequencing showed altered gut microbiota, with enrichment of the butyrate-producing species Anaerostipes hominis and Bifidobacterium pseudolongum in the FHB group. These findings indicate that FHB supplementation was associated with attenuated metabolic dysfunction and preserved muscle function, accompanied by fermentation-derived metabolites and gut microbiota remodeling.
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BibTeX
@article{kuo2026lactiplantibacillus,
title = {{Lactiplantibacillus plantarum-Fermented Broccoli Attenuates Metabolic Dysfunction and Improves Skeletal Muscle Quality in High-Fat/High-Fructose Diet-Fed Mice.}},
author = {Ya-Ru Kuo and Tzu-Ling Lin and Pin-Yu Ho and Wei-Jen Chen and Hui-Fang Chu and Ming-Chung Tseng and Yu-Shan Wei and Min-Hsiung Pan},
journal = {Journal of agricultural and food chemistry},
year = {2026},
month = oct,
doi = {10.1021/acs.jafc.6c05322},
url = {https://europepmc.org/article/MED/42915814},
note = {CC BY},
}
RIS
TY - JOUR
TI - Lactiplantibacillus plantarum-Fermented Broccoli Attenuates Metabolic Dysfunction and Improves Skeletal Muscle Quality in High-Fat/High-Fructose Diet-Fed Mice.
AU - Ya-Ru Kuo
AU - Tzu-Ling Lin
AU - Pin-Yu Ho
AU - Wei-Jen Chen
AU - Hui-Fang Chu
AU - Ming-Chung Tseng
AU - Yu-Shan Wei
AU - Min-Hsiung Pan
PY - 2026
DA - 2026/10/01
JO - Journal of agricultural and food chemistry
DO - 10.1021/acs.jafc.6c05322
UR - https://europepmc.org/article/MED/42915814
AB - Muscle loss accompanied by excessive adiposity is a critical component of metabolic dysfunction. This study investigated the effects of Lactiplantibacillus plantarum-fermented broccoli (FHB) in high-fat/high-fructose diet (HFFD)-fed C57BL/6J mice. LC-MS profiling showed that fermentation generated metabolites, including 2-hydroxycaproic acid, 3-phenyllactic acid, and a tetrahydro-β-carboline derivative. FHB supplementation attenuated visceral adiposity, hepatic steatosis, and insulin resistance while preserving grip strength. In the gastrocnemius muscle, FHB was associated with higher phosphorylation of the IRS1/PI3K/Akt and mTOR/p70S6K/4E-BP1 pathways and with lower expression of the ubiquitin ligases Atrogin-1 and MuRF-1. 16S rRNA sequencing showed altered gut microbiota, with enrichment of the butyrate-producing species Anaerostipes hominis and Bifidobacterium pseudolongum in the FHB group. These findings indicate that FHB supplementation was associated with attenuated metabolic dysfunction and preserved muscle function, accompanied by fermentation-derived metabolites and gut microbiota remodeling.
SN - 0021-8561
SN - 1520-5118
ER -