Phylogeny and reassessment of the genus Tolypocladium s. lato.: Proposing a new genus, three new species and two combinations.
5 affiliations
- 1Center for Mycomedicine Research, Basic Medical School, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, Guizhou Province, China Center for Mycomedicine Research, Basic Medical School, Guizhou University of Traditional Chinese Medicine Guiyang China.
- 2Institute of Fungus Resources, Department of Ecology, College of Life Sciences, Guizhou University, Guiyang 550025, Guizhou Province, China Institute of Fungus Resources, Department of Ecology, College of Life Sciences, Guizhou University Guiyang China.
- 3Key Laboratory of Microbiome and Infectious Disease Prevention & Control in Guizhou Province, Guiyang 550025, Guizhou Province, China Key Laboratory of Microbiome and Infectious Disease Prevention & Control in Guizhou Province Guiyang China.
- 4College of Pharmacy, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, Guizhou Province, China College of Pharmacy, Guizhou University of Traditional Chinese Medicine Guiyang China.
- 5Department of Bioprocess Technology, Faculty of Technology, Rajarata University of Sri Lanka, Mihintale 50300, Sri Lanka Department of Bioprocess Technology, Faculty of Technology, Rajarata University of Sri Lanka Mihintale Sri Lanka.
Abstract
This study presents a phylogenetic reassessment and taxonomic revision of the genus Tolypocladium s. str. in Ophiocordycipitaceae. A phylogenetic analysis based on five loci (ITS, LSU, rpb1, rpb2, and tef-1α) was conducted, combined with morphological characters and host associations. Traditionally, Tolypocladium species are regarded as polyphyletic, with species distributed across three families of Hypocreales: Ophiocordycipitaceae, Cordycipitaceae, and Clavicipitaceae. Consequently, we propose a new genus, Tolypocladiopsis, to accommodate Tolypocladium extinguens (in Cordycipitaceae); describe three new species, Tolypocladium guizhouense, T. tongrenense, and T. zongqii, from Fanjing Mountain, Guizhou Province, China. Tolypocladium lignicola is accommodated in Metapochonia s. str., and we accordingly introduce M. lignicola. Our findings refine the generic delimitation of Tolypocladium, resolve long-standing taxonomic ambiguities, underscore the misleading nature of morphological convergence in hypocrealean anamorphs, and advocate for an integrative taxonomy that combines molecular phylogenetics with detailed morphological examination.
Related
- Reassessment of Podosphaera carpesiicola: Host Associations, Holomorph Morphology, and Molecular Phylogeny.
- Type-Based Morphological Reassessment of Nola yoshinensis and N. kanshireiensis (Lepidoptera, Nolidae), with Description of a Previously Misidentified New Species in Asia †
- Phylogeny and Species Delimitation in Isoxylosteum, a Lonicera Clade Endemic to the Himalayan-Tibetan-Hengduan Region
- Phylogeny and comparative chloroplast genomics reveal a rare new short-styled homostylous species of Primula (Primulaceae) from southwestern China
- A new Oriental Agalliini leafhopper genus (Hemiptera, Cicadellidae, Megophthalminae), with description of one new species and three new combinations
BibTeX
@article{chen2026phylogeny,
title = {{Phylogeny and reassessment of the genus Tolypocladium s. lato.: Proposing a new genus, three new species and two combinations.}},
author = {Wan-Hao Chen and Chuan-Xuan Xiong and Hui-Lin Shu and Dan Li and Qian Zeng and Jian-Dong Liang and Nalin N Wijayawardene and Yan-Feng Han and Jie-Hong Zhao},
journal = {MycoKeys},
year = {2026},
month = oct,
doi = {10.3897/mycokeys.141.204222},
url = {https://europepmc.org/article/MED/42909249},
note = {CC BY},
}
RIS
TY - JOUR
TI - Phylogeny and reassessment of the genus Tolypocladium s. lato.: Proposing a new genus, three new species and two combinations.
AU - Wan-Hao Chen
AU - Chuan-Xuan Xiong
AU - Hui-Lin Shu
AU - Dan Li
AU - Qian Zeng
AU - Jian-Dong Liang
AU - Nalin N Wijayawardene
AU - Yan-Feng Han
AU - Jie-Hong Zhao
PY - 2026
DA - 2026/10/13
JO - MycoKeys
DO - 10.3897/mycokeys.141.204222
UR - https://europepmc.org/article/MED/42909249
AB - This study presents a phylogenetic reassessment and taxonomic revision of the genus Tolypocladium s. str. in Ophiocordycipitaceae. A phylogenetic analysis based on five loci (ITS, LSU, rpb1, rpb2, and tef-1α) was conducted, combined with morphological characters and host associations. Traditionally, Tolypocladium species are regarded as polyphyletic, with species distributed across three families of Hypocreales: Ophiocordycipitaceae, Cordycipitaceae, and Clavicipitaceae. Consequently, we propose a new genus, Tolypocladiopsis, to accommodate Tolypocladium extinguens (in Cordycipitaceae); describe three new species, Tolypocladium guizhouense, T. tongrenense, and T. zongqii, from Fanjing Mountain, Guizhou Province, China. Tolypocladium lignicola is accommodated in Metapochonia s. str., and we accordingly introduce M. lignicola. Our findings refine the generic delimitation of Tolypocladium, resolve long-standing taxonomic ambiguities, underscore the misleading nature of morphological convergence in hypocrealean anamorphs, and advocate for an integrative taxonomy that combines molecular phylogenetics with detailed morphological examination.
SN - 1314-4057
SN - 1314-4049
ER -