Type 6 secretion systems of adherent-invasive Escherichia coli isolated from Crohn's disease patients shape intestinal microbiota.
- 1UMR 1071 M2iSH (Microbes, Intestine, Inflammation and Susceptibility of the Host), Clermont Auvergne University, Inserm, INRAe USC 1382, Clermont-Ferrand, Franc
Abstract
Adherent Invasive Escherichia coli (AIEC) colonize the ileal mucosa of Crohn's disease patients. Among bacterial virulence factors potentially implicated, type 6 secretion systems (T6SS) are molecular machineries sending effectors in prokaryotic or eukaryotic target cells. Two T6SS have been reported in AIEC LF82 reference strain. Here, we show that T6SS genes are more prevalent in Crohn's disease patients' microbiota compared to healthy controls, and that T6SS are more frequent in AIEC pathovar compared to E. coli in general and associated with FimH pathoadaptive polymorphism. The use of an in vitro gastrointestinal system coupled with T6SS promoter reporter assay highlighted expression of LF82 T6SS under gastrointestinal conditions, with a major role for bile salts. In mice infected with AIEC LF82 versus T6SS-deleted mutants, T6SS showed a functional impact in modulating the composition of mucosa-associated gut microbiota. Thus, AIEC may use their T6SS to compete with endogenous microbiota to colonize host mucosa.
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BibTeX
@misc{botchorichvili2026type,
title = {{Type 6 secretion systems of adherent-invasive Escherichia coli isolated from Crohn's disease patients shape intestinal microbiota.}},
author = {H. Botchorichvili and S. Massier and B. Chassaing and A. Birer and C. Chevarin and G. Sevrin and M. Rodrigues and S. Denis and I. Wawrzyniak and E. Peyretaillade and R. Bonnet and B. Pereira and M. Allez and P. Seksik and J. Denizot and The Remind Group and A. Buisson and L. Journet and N. Barnich and E. BILLARD},
howpublished = {bioRxiv},
year = {2026},
month = oct,
doi = {10.64898/2026.10.08.757420},
url = {https://www.biorxiv.org/content/10.64898/2026.10.08.757420v1},
note = {CC BY},
}
RIS
TY - UNPB
TI - Type 6 secretion systems of adherent-invasive Escherichia coli isolated from Crohn's disease patients shape intestinal microbiota.
AU - H. Botchorichvili
AU - S. Massier
AU - B. Chassaing
AU - A. Birer
AU - C. Chevarin
AU - G. Sevrin
AU - M. Rodrigues
AU - S. Denis
AU - I. Wawrzyniak
AU - E. Peyretaillade
AU - R. Bonnet
AU - B. Pereira
AU - M. Allez
AU - P. Seksik
AU - J. Denizot
AU - The Remind Group
AU - A. Buisson
AU - L. Journet
AU - N. Barnich
AU - E. BILLARD
PY - 2026
DA - 2026/10/08
PB - bioRxiv
DO - 10.64898/2026.10.08.757420
UR - https://www.biorxiv.org/content/10.64898/2026.10.08.757420v1
AB - Adherent Invasive Escherichia coli (AIEC) colonize the ileal mucosa of Crohn's disease patients. Among bacterial virulence factors potentially implicated, type 6 secretion systems (T6SS) are molecular machineries sending effectors in prokaryotic or eukaryotic target cells. Two T6SS have been reported in AIEC LF82 reference strain. Here, we show that T6SS genes are more prevalent in Crohn's disease patients' microbiota compared to healthy controls, and that T6SS are more frequent in AIEC pathovar compared to E. coli in general and associated with FimH pathoadaptive polymorphism. The use of an in vitro gastrointestinal system coupled with T6SS promoter reporter assay highlighted expression of LF82 T6SS under gastrointestinal conditions, with a major role for bile salts. In mice infected with AIEC LF82 versus T6SS-deleted mutants, T6SS showed a functional impact in modulating the composition of mucosa-associated gut microbiota. Thus, AIEC may use their T6SS to compete with endogenous microbiota to colonize host mucosa.
ER -