Functional genomic analysis of Vreelandella zhaodongensis BS253: a new isolate from hypersaline lakes from Brazilian Pantanal
- 1Institute of Petroleum and Natural Resources (IPR), Pontifical Catholic University of Rio Grande do Sul (PUCRS), Porto Alegre, Brazil
- 2Graduate Program in Cellular and Molecular Biology, Center of Biotechnology, Federal University of Rio Grande do Sul, Porto Alegre, Brazil
Abstract
Natural ecosystems harbor an immense diversity of microorganisms whose metabolic capabilities remain unexplored. Therefore, the investigation of microbial life in diverse environments can reveal bacteria with unique physiological and biochemical traits, which may be useful for biotechnological applications. In this study, we report the complete genome sequencing and functional characterization of isolate BS253, isolated in a hypersaline alkaline lake from Brazil’s Pantanal region. Using a hybrid sequencing strategy combining Oxford Nanopore long reads and Illumina short reads, we assembled a circular chromosome of 3.76 Mb and two plasmids. Phylogenetic and comparative genomic analyses identified the isolate as Vreelandella zhaodongensis. Digital DNA-DNA hybridization (76.4%) and ANI (96.83%) values supported the designation of BS253 as a strain of V. zhaodongensis. Genome mining revealed genes potentially associated with salt and alkali tolerance, hydrocarbon and plastic degradation, and the biosynthesis of secondary metabolites. Phenotypically, BS253 is a moderately halophilic, facultatively anaerobic, Gram-negative rod that also exhibits emulsifying activity (55.1%) under defined culture conditions. These findings highlight strain BS253 as a metabolically versatile extremophile and a promising candidate for future studies in environmental and industrial biotechnology.
Supplementary Information: The online version contains supplementary material available at https://doi.org/10.1007/s00203-026-05318-2.
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BibTeX
@article{lautertdutra2026functional,
title = {{Functional genomic analysis of Vreelandella zhaodongensis BS253: a new isolate from hypersaline lakes from Brazilian Pantanal}},
author = {William Lautert-Dutra and Amanda Pasinato Napp and Eduardo Alexandre Back Sivinski and Charley Christian Staats and Francine Melise dos Santos and Clarissa Lovato Melo},
journal = {Archives of Microbiology},
year = {2026},
month = oct,
doi = {10.1007/s00203-026-05318-2},
url = {https://doi.org/10.1007/s00203-026-05318-2},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Functional genomic analysis of Vreelandella zhaodongensis BS253: a new isolate from hypersaline lakes from Brazilian Pantanal
AU - William Lautert-Dutra
AU - Amanda Pasinato Napp
AU - Eduardo Alexandre Back Sivinski
AU - Charley Christian Staats
AU - Francine Melise dos Santos
AU - Clarissa Lovato Melo
PY - 2026
DA - 2026/10/31
JO - Archives of Microbiology
DO - 10.1007/s00203-026-05318-2
UR - https://doi.org/10.1007/s00203-026-05318-2
AB - Natural ecosystems harbor an immense diversity of microorganisms whose metabolic capabilities remain unexplored. Therefore, the investigation of microbial life in diverse environments can reveal bacteria with unique physiological and biochemical traits, which may be useful for biotechnological applications. In this study, we report the complete genome sequencing and functional characterization of isolate BS253, isolated in a hypersaline alkaline lake from Brazil’s Pantanal region. Using a hybrid sequencing strategy combining Oxford Nanopore long reads and Illumina short reads, we assembled a circular chromosome of 3.76 Mb and two plasmids. Phylogenetic and comparative genomic analyses identified the isolate as Vreelandella zhaodongensis. Digital DNA-DNA hybridization (76.4%) and ANI (96.83%) values supported the designation of BS253 as a strain of V. zhaodongensis. Genome mining revealed genes potentially associated with salt and alkali tolerance, hydrocarbon and plastic degradation, and the biosynthesis of secondary metabolites. Phenotypically, BS253 is a moderately halophilic, facultatively anaerobic, Gram-negative rod that also exhibits emulsifying activity (55.1%) under defined culture conditions. These findings highlight strain BS253 as a metabolically versatile extremophile and a promising candidate for future studies in environmental and industrial biotechnology. Supplementary Information: The online version contains supplementary material available at https://doi.org/10.1007/s00203-026-05318-2.
SN - 0302-8933
SN - 1432-072X
ER -