Extended DNA damage-induced G2/M arrests lead to aberrant mitoses through accumulation of securin.
- 1Institute of Cell Biology, University of Edinburgh , Edinburgh, UK.
Abstract
The stochastic nature of DNA damage dictates various scenarios of stress survival among different cells. We used time-lapse microscopy to perform single-cell level analyses of yeast populations with double-stranded DNA breaks and nonfunctional telomeres. Activation of the DNA damage signalling resulted in a broad distribution in the duration of G2/M arrests. Strikingly, the longer arrests correlated with aberrant mitoses caused by miscoordination of nuclear division and cytokinesis, leading to cell death. Chk1-dependent phosphorylation of Pds1 (securin), an inhibitor of sister chromatid separation in mitosis, was responsible for this phenomenon. Securin progressively accumulated during G2/M arrests, leading to grossly delayed or missed nuclear divisions. This phenotype could be suppressed by slowing down the progression of mitotic exit via LTE1 deletion or by lowering securin levels. Our results demonstrate that in cells taking too long to complete DNA repair, the DNA damage checkpoint promotes aberrant mitoses and cell death, thereby eliminating cells with a higher chance of genomic instability.
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BibTeX
@article{dubenko2026extended,
title = {{Extended DNA damage-induced G2/M arrests lead to aberrant mitoses through accumulation of securin.}},
author = {Anton Dubenko and Marah Jnied and Oleksii Kotenko and Svetlana Makovets},
journal = {The Journal of cell biology},
year = {2026},
month = nov,
doi = {10.1083/jcb.202511106},
url = {https://europepmc.org/article/MED/42969970},
note = {CC BY},
}
RIS
TY - JOUR
TI - Extended DNA damage-induced G2/M arrests lead to aberrant mitoses through accumulation of securin.
AU - Anton Dubenko
AU - Marah Jnied
AU - Oleksii Kotenko
AU - Svetlana Makovets
PY - 2026
DA - 2026/11/02
JO - The Journal of cell biology
DO - 10.1083/jcb.202511106
UR - https://europepmc.org/article/MED/42969970
AB - The stochastic nature of DNA damage dictates various scenarios of stress survival among different cells. We used time-lapse microscopy to perform single-cell level analyses of yeast populations with double-stranded DNA breaks and nonfunctional telomeres. Activation of the DNA damage signalling resulted in a broad distribution in the duration of G2/M arrests. Strikingly, the longer arrests correlated with aberrant mitoses caused by miscoordination of nuclear division and cytokinesis, leading to cell death. Chk1-dependent phosphorylation of Pds1 (securin), an inhibitor of sister chromatid separation in mitosis, was responsible for this phenomenon. Securin progressively accumulated during G2/M arrests, leading to grossly delayed or missed nuclear divisions. This phenotype could be suppressed by slowing down the progression of mitotic exit via LTE1 deletion or by lowering securin levels. Our results demonstrate that in cells taking too long to complete DNA repair, the DNA damage checkpoint promotes aberrant mitoses and cell death, thereby eliminating cells with a higher chance of genomic instability.
SN - 0021-9525
SN - 1540-8140
ER -